87 research outputs found
Synapsis and Meiotic Recombination in Male Chinese Muntjac (Muntiacus reevesi)
The muntjacs (Muntiacus, Cervidae) have been extensively studied in terms of chromosomal and karyotypic evolution. However, little is known about their meiotic chromosomes particularly the recombination patterns of homologous chromosomes. We used immunostained surface spreads to visualise synaptonemal complexes (SCs), recombination foci and kinetochores with antibodies against marker proteins. As in other mammals pachytene was the longest stage of meiotic prophase. 39.4% of XY bivalents lacked MLH1 foci compared to less than 0.5% of autosomes. The average number of MLH1 foci per pachytene cell in M. reevesi was 29.8. The distribution of MLH1 foci differed from other mammals. On SCs with one focus, the distribution was more even in M. reevesi than in other mammals; for SCs that have two or more MLH1 foci, usually there was a larger peak in the sub-centromere region than other regions on SC in M. reevesi. Additionally, there was a lower level of interference between foci in M. reevesi than in mouse or human. These observations may suggest that the regulation of homologous recombination in M. reevesi is slightly different from other mammals and will improve our understanding of the regulation of meiotic recombination, with respect to recombination frequency and position
The effects of a diet formulation with oats, soybeans, and flax on lipid profiles and uricemia in patients with AIDS and dyslipidemia
Broad-Scale Recombination Patterns Underlying Proper Disjunction in Humans
Although recombination is essential to the successful completion of human meiosis, it remains unclear how tightly the process is regulated and over what scale. To assess the nature and stringency of constraints on human recombination, we examined crossover patterns in transmissions to viable, non-trisomic offspring, using dense genotyping data collected in a large set of pedigrees. Our analysis supports a requirement for one chiasma per chromosome rather than per arm to ensure proper disjunction, with additional chiasmata occurring in proportion to physical length. The requirement is not absolute, however, as chromosome 21 seems to be frequently transmitted properly in the absence of a chiasma in females, a finding that raises the possibility of a back-up mechanism aiding in its correct segregation. We also found a set of double crossovers in surprisingly close proximity, as expected from a second pathway that is not subject to crossover interference. These findings point to multiple mechanisms that shape the distribution of crossovers, influencing proper disjunction in humans
Genome-Wide Crossover Distribution in Arabidopsis thaliana Meiosis Reveals Sex-Specific Patterns along Chromosomes
In most species, crossovers (COs) are essential for the accurate segregation of homologous chromosomes at the first meiotic division. Their number and location are tightly regulated. Here, we report a detailed, genome-wide characterization of the rate and localization of COs in Arabidopsis thaliana, in male and female meiosis. We observed dramatic differences between male and female meiosis which included: (i) genetic map length; 575 cM versus 332 cM respectively; (ii) CO distribution patterns: male CO rates were very high at both ends of each chromosome, whereas female CO rates were very low; (iii) correlations between CO rates and various chromosome features: female CO rates correlated strongly and negatively with GC content and gene density but positively with transposable elements (TEs) density, whereas male CO rates correlated positively with the CpG ratio. However, except for CpG, the correlations could be explained by the unequal repartition of these sequences along the Arabidopsis chromosome. For both male and female meiosis, the number of COs per chromosome correlates with chromosome size expressed either in base pairs or as synaptonemal complex length. Finally, we show that interference modulates the CO distribution both in male and female meiosis
Moments of the Position of the Maximum for GUE Characteristic Polynomials and for Log-Correlated Gaussian Processes
We study three instances of log-correlated processes on the interval: the
logarithm of the Gaussian unitary ensemble (GUE) characteristic polynomial, the
Gaussian log-correlated potential in presence of edge charges, and the
Fractional Brownian motion with Hurst index (fBM0). In previous
collaborations we obtained the probability distribution function (PDF) of the
value of the global minimum (equivalently maximum) for the first two processes,
using the {\it freezing-duality conjecture} (FDC). Here we study the PDF of the
position of the maximum through its moments. Using replica, this requires
calculating moments of the density of eigenvalues in the -Jacobi
ensemble. Using Jack polynomials we obtain an exact and explicit expression for
both positive and negative integer moments for arbitrary and
positive integer in terms of sums over partitions. For positive moments,
this expression agrees with a very recent independent derivation by Mezzadri
and Reynolds. We check our results against a contour integral formula derived
recently by Borodin and Gorin (presented in the Appendix A from these authors).
The duality necessary for the FDC to work is proved, and on our expressions,
found to correspond to exchange of partitions with their dual. Performing the
limit and to negative Dyson index , we obtain the
moments of and give explicit expressions for the lowest ones. Numerical
checks for the GUE polynomials, performed independently by N. Simm, indicate
encouraging agreement. Some results are also obtained for moments in Laguerre,
Hermite-Gaussian, as well as circular and related ensembles. The correlations
of the position and the value of the field at the minimum are also analyzed.Comment: 64 page, 5 figures, with Appendix A written by Alexei Borodin and
Vadim Gorin; The appendix H in the ArXiv version is absent in the published
versio
Cytological basis of sterility in male and female hybrids between sibling species of grey voles Microtus arvalis and M. levis
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