Effect of solvent composition on DSC exothermic peak of human serum albumin suspended in pyridkne-n-hexane mixtures

Human serum albumin (HSA) immersed in pyridine-n-hexane mixtures was analyzed using differential scanning calorimetry (DSC). State of the solid HSA in organic solvent mixtures is the non-equilibrium state which is seen as the exothermic peak on the DSC curves. The enthalpy change corresponding to this exothermic peak approaches zero when going from pure pyridine to pure n-hexane. Dependence of the enthalpy change on the pyridine concentration is suggestive that the non-equilibrium state of the immersed HSA results from the HSA-pyridine interactions 'frozen' at the lower temperature. Most likely the temperature-initiated exothermic peak observed on the DSC curves reflects the swelling of HSA by pyridine

Hydrogen bonds formed by methyl groups of acetonitrile: Infrared and calorimetric study

Solutions of acetonitrile (I) in tetrachloromethane and deuteratred solvents (S) (benzene, acctonitrile, acetone and dimethylsulphoxide) have been studied by IR absorption spectra. The observed solvent effect on the IR spectrum of I was explained in terms of the existence of complexes with hydrogen bonding of the type NCCH3...solvent (S). The strength of the hydrogen bonding was characterized by enthalpies of specific interactions of I with solvents ΔHI/S int(sp). The values ΔHI/S int(sp) were determined both by IR spectroscopy and calorimetry and were found to be within the range 0.3-1.5 kcal mol-1. © 1993

Effect of solvent composition on DSC exothermic peak of human serum albumin suspended in pyridkne-n-hexane mixtures

Human serum albumin (HSA) immersed in pyridine-n-hexane mixtures was analyzed using differential scanning calorimetry (DSC). State of the solid HSA in organic solvent mixtures is the non-equilibrium state which is seen as the exothermic peak on the DSC curves. The enthalpy change corresponding to this exothermic peak approaches zero when going from pure pyridine to pure n-hexane. Dependence of the enthalpy change on the pyridine concentration is suggestive that the non-equilibrium state of the immersed HSA results from the HSA-pyridine interactions 'frozen' at the lower temperature. Most likely the temperature-initiated exothermic peak observed on the DSC curves reflects the swelling of HSA by pyridine

Effect of solvent composition on DSC exothermic peak of human serum albumin suspended in pyridkne-n-hexane mixtures

Human serum albumin (HSA) immersed in pyridine-n-hexane mixtures was analyzed using differential scanning calorimetry (DSC). State of the solid HSA in organic solvent mixtures is the non-equilibrium state which is seen as the exothermic peak on the DSC curves. The enthalpy change corresponding to this exothermic peak approaches zero when going from pure pyridine to pure n-hexane. Dependence of the enthalpy change on the pyridine concentration is suggestive that the non-equilibrium state of the immersed HSA results from the HSA-pyridine interactions 'frozen' at the lower temperature. Most likely the temperature-initiated exothermic peak observed on the DSC curves reflects the swelling of HSA by pyridine

Effect of solvent composition on DSC exothermic peak of human serum albumin suspended in pyridkne-n-hexane mixtures

Human serum albumin (HSA) immersed in pyridine-n-hexane mixtures was analyzed using differential scanning calorimetry (DSC). State of the solid HSA in organic solvent mixtures is the non-equilibrium state which is seen as the exothermic peak on the DSC curves. The enthalpy change corresponding to this exothermic peak approaches zero when going from pure pyridine to pure n-hexane. Dependence of the enthalpy change on the pyridine concentration is suggestive that the non-equilibrium state of the immersed HSA results from the HSA-pyridine interactions 'frozen' at the lower temperature. Most likely the temperature-initiated exothermic peak observed on the DSC curves reflects the swelling of HSA by pyridine

Hydrogen bonds formed by methyl groups of acetonitrile: Infrared and calorimetric study

Solutions of acetonitrile (I) in tetrachloromethane and deuteratred solvents (S) (benzene, acctonitrile, acetone and dimethylsulphoxide) have been studied by IR absorption spectra. The observed solvent effect on the IR spectrum of I was explained in terms of the existence of complexes with hydrogen bonding of the type NCCH3...solvent (S). The strength of the hydrogen bonding was characterized by enthalpies of specific interactions of I with solvents ΔHI/S int(sp). The values ΔHI/S int(sp) were determined both by IR spectroscopy and calorimetry and were found to be within the range 0.3-1.5 kcal mol-1. © 1993

Hydrogen bonds formed by methyl groups of acetonitrile: Infrared and calorimetric study

Solutions of acetonitrile (I) in tetrachloromethane and deuteratred solvents (S) (benzene, acctonitrile, acetone and dimethylsulphoxide) have been studied by IR absorption spectra. The observed solvent effect on the IR spectrum of I was explained in terms of the existence of complexes with hydrogen bonding of the type NCCH3...solvent (S). The strength of the hydrogen bonding was characterized by enthalpies of specific interactions of I with solvents ΔHI/S int(sp). The values ΔHI/S int(sp) were determined both by IR spectroscopy and calorimetry and were found to be within the range 0.3-1.5 kcal mol-1. © 1993

Hydrogen bonds formed by methyl groups of acetonitrile: Infrared and calorimetric study

Solutions of acetonitrile (I) in tetrachloromethane and deuteratred solvents (S) (benzene, acctonitrile, acetone and dimethylsulphoxide) have been studied by IR absorption spectra. The observed solvent effect on the IR spectrum of I was explained in terms of the existence of complexes with hydrogen bonding of the type NCCH3...solvent (S). The strength of the hydrogen bonding was characterized by enthalpies of specific interactions of I with solvents ΔHI/S int(sp). The values ΔHI/S int(sp) were determined both by IR spectroscopy and calorimetry and were found to be within the range 0.3-1.5 kcal mol-1. © 1993