A genome-wide genetic map of NB-LRR disease resistance loci in potato

Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato

Basalt‐polypropylene fiber reinforced concrete for durable and sustainable pipe production. Part 1: Experimental program

This is the peer reviewed version of the following article: [ Deng, Z, Liu, X, Chen, P, et al. Basalt-polypropylene fiber reinforced concrete for durable and sustainable pipe production. Part 1: Experimental program. Structural Concrete. 2022; 23: 311– 327. https://doi.org/10.1002/suco.202000759], which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/suco.202000759. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.An experimental program consisting in producing and testing reinforced concrete pipes (RCPs) under the three-edge bearing tests considering different types of reinforcement was carried out. Four types of RCPs were produced, these reinforced with: (1) polypropylene macrofibers; (2) basalt microfibers; (3) combination of both (hybrid reinforcement); and (4) plain concrete. The analysis of the crack patterns and both service and ultimate mechanical responses allowed concluding that the use of fibers do not lead to an effective increase of the first cracking load; however, both types of fibers allowed a better crack width control respect to the standard RCP. In this regard, basalt microfiber reinforced concrete led to a better response caused by concentrated loads (jacketing) whilst polypropylene macrofibers increased the concrete pipe performance in terms of bearing capacity and flexural crack control. The hybrid fiber reinforced concrete was found to be the most suitable alternative for increasing the load bearing capacity and the crack width control for service loads. These incipient experimental results permit to conclude that this type of hybrid basalt-polypropylene fiber reinforced concretes are an interesting alternative to traditional steel-cage RCPs.This work is supported by the National Key Research and Development Program of China (2018YFC1504802), Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (cstc2018jscxmszdX0071), Postgraduate Research Innovation Project of Chongqing (CYS19005, CYS18026). In addition, Prof. Albert de la Fuente also wants to express his gratitude to the Spanish Ministry of Science and Innovation for the financial support received under the scope of the project CREEF (PID2019-108978RB-C32).Peer ReviewedPostprint (author's final draft