Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells.

Cellular products derived from the activity of DNA, RNA, and protein synthesis collectively control cell identity and function. Yet there is little information on how these three biosynthesis activities are coordinated during transient and sparse cellular processes, such as activation and differentiation. Here, we describe Simultaneous Overview of tri-Molecule Biosynthesis (SOM3B), a molecular labeling and simultaneous detection strategy to quantify DNA, RNA, and protein synthesis in individual cells. Comprehensive interrogation of biosynthesis activities during transient cell states, such as progression through cell cycle or cellular differentiation, is achieved by partnering SOM3B with parallel quantification of select biomolecules with conjugated antibody reagents. Here, we investigate differential de novo DNA, RNA, and protein synthesis dynamics in transformed human cell lines, primary activated human immune cells, and across the healthy human hematopoietic continuum, all at a single-cell resolution

The 1980, 1997 and 1998 Azores earthquakes and its seismotectonic implications

We have studied the focal mechanisms of the 1980, 1997 and 1998 earthquakes in the Azores region from body-wave inversion of digital GDSN (Global Digital Seismograph Network) and broadband data. For the 1980 and 1998 shocks, we have obtained strike– slip faulting, with the rupture process made up of two sub-events in both shocks, with total scalar seismic moments of 1.9 × 1019 Nm (Mw = 6.8) and 1.4 × 1018 Nm (Mw = 6.0), respectively. For the 1997 shock, we have obtained a normal faulting mechanism, with the rupture process made up of three sub-events, with a total scalar seismic moment of 7.7 × 1017 Nm (Mw = 5.9). A common characteristic of these three earthquakes was the shallow focal depth, less than 10 km, in agreement with the oceanic-type crust. From the directivity function of Rayleigh (LR) waves, we have identified the NW–SE plane as the rupture plane for the 1980 and 1998 earthquakes with the rupture propagating to the SE. Slow rupture velocity, about of 1.5 km/s, has been estimated from directivity function for the 1980 and 1998 earthquakes. From spectral analysis and body-wave inversion, fault dimensions, stress drop and average slip have been estimated. Focal mechanisms of the three earthquakes we have studied, together with focal mechanisms obtained by other authors, have been used in order to obtain a seismotectonic model for the Azores region. We have found different types of behaviour present along the region. It can be divided into two zones: Zone I, from 30°W to 27°W; Zone II, from 27°W to 23°W, with a change in the seismicity and stress direction from Zone I. In Zone I, the total seismic moment tensor obtained corresponded to left-lateral strike–slip faulting with horizontal pressure and tension axes in the E–W and N–S directions, respectively. In Zone II, the total seismic moment tensor corresponded to normal faulting, with a horizontal tension axis trending NE–SW, normal to the Terceira Ridge. The stress pattern for the whole region corresponds to horizontal extension with an average seismic slip rate of 4.4 mm/yr