Nanolocalized single-cell-membrane nanoelectroporation: For higher efficiency with high cell viability

TODAY, SINGLE-CELL RESEARCH is of great interest to analyze cell-to-cell or cell-to-environment behavior with their intracellular compounds, where bulk measurements of millions of cells together can provide an average value. To deliver biomolecules in a precise and localized way into single living cells with a high transfection rate and high cell viability is a challenging and promisible task for biological and therapeutic research. © 2007-2011 IEEE

Interface behaviour from suction controlled direct shear test on completely decomposed granitic soil and steel surfaces

A soil-structure interface is defined as the contact surface between a soil and a structure through which stresses are transferred from the soil to the structure or vice versa. The ultimate shear strength at the interface is one of the key parameters required for the design and safety assessment of a structure in the soil, such as pile foundations, retaining walls, buried pipelines, and soil nails. In this paper, the shearing behavior of completely decomposed granite soil and steel interfaces is examined using a modified suction-controlled direct shear apparatus. A series of direct shear tests was performed on two different types of soil-steel interfaces under different stress state variables. The experimental results were used to investigate the influence of counterface roughness on the failure envelopes of soil-steel interfaces. Test results show that matric suction has a significant influence on the shear behavior and shear strength of the interfaces. It has been observed that the critical interface shear strength for a specific counterface roughness and net normal stress (NNS) depends on the applied matric suction. Also, both the peak and the postpeak shear stresses are greatly influenced by variation in NNS. Furthermore, the experimental shear strength data are compared with an analytical model that considers the influence of suction and dilation on an apparent interface friction angle. It is noted that, for the applied NNS and matric suction, the analytical model works well for both rough interfaces

Nanolocalized single cell membrane nanoelectroporation

Today single cell research is a great interest to analyze cell to cell or cell to environment behavior with their intracellular compounds, where bulk measurement can provide average value. To deliver biomolecules precise and localized way into single living cell with high transfection rate and high cell viability is a challenging and promisible task for biological and therapeutic research. In this report, we present a nano-localized single cell nano-electroporation technique, where electroporation take place in a very precise and localized area on a single cell membrane to achieve high efficient delivery with high cell viability. We fabricated 60nm gap with 40 nm triangular Indium Tin Oxide (ITO) based nano-eletcrode tip, which can intense electric field in a nano-localized area of a single cell to permeabilize cell membrane and deliver exogenous biomolecules from outside to inside of the cell. This device successfully deliver dyes, proteins into single cell with high cell viability (98%). The process not only control precise delivery mechanism into single cell with membrane reversibility, but also it can provide special, temporal and qualitative dosage control, which might be beneficial for therapeutic and biological cell studies

Near-infrared nanosecond-pulsed laser-activated highly efficient intracellular delivery mediated by nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles

Here, an efficient intracellular delivery of molecules with high cell viability is reported using nanosecond-pulsed laser-activated plasmonic photoporation, mediated by high-aspect-ratio nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles (nm-AuPNPs) at near-infrared wavelength. Upon pulsed laser illumination, nm-AuPNPs exhibit greater plasmonic extinction than spherical AuPNPs, which increase their energy efficiency and reduce the necessary illumination of light, effectively controlling cell damage and improving the delivery efficiency. Nm-AuPNPs exhibit surface plasmon absorption at near infrared region with a peak at 945 nm. Pulsed laser illumination at this plasmon peak triggers explosive nanobubbles, which create transient membrane pores, allowing the delivery of dyes, quantum dots and plasmids into the different cell types. The results can be tuned by laser fluence, exposure time, molecular size and concentration of nm-AuPNPs. The best results are found for CL1-0 cells, which yielded a 94% intracellular PI dye uptake and ~100% cell viability at 35 mJ cm-2 laser fluence for 945 nm wavelength. Thus, the presented approach has proven to have an inevitable potential for biological cell research and therapeutic applications

Designing and implementing a research integrity promotion plan: Recommendations for research funders

AVUari:ouPslesatsaekceohnofilrdmetrhsaitnalslchieeandcineghleavveelspauret rreepsreeasrecnhteidncteogrrrietyctlhyi:gh on their agenda. Among them, research funders are prominently placed to foster research integrity by requiring that the organizations and individual researchers they support make an explicit commitment to research integrity. Moreover, funders need to adopt appropriate research integrity practices themselves. To facilitate this, we recommend that funders develop and implement a Research Integrity Promotion Plan (RIPP). This Consensus View offers a range of examples of how funders are already promoting research integrity, distills 6 core topics that funders should cover in a RIPP, and provides guidelines on how to develop and implement a RIPP. We believe that the 6 core topics we put forward will guide funders towards strengthening research integrity policy in their organization and guide the researchers and research organizations they fund