A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars

<p>Abstract</p> <p>Background</p> <p>Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typically performed on cumbersome polyacrylamide gels or more conveniently by capillary electrophoresis on automated sequencers. However post-PCR handling steps are still required. High resolution melting can now combine detailed sequence analysis with the closed-tube benefits of real-time PCR and is described here as a novel way to verify the identity of plant varieties such as grapevine and olive.</p> <p>Results</p> <p>DNA melting profiles for various plant variety and rootstock samples were compared to profiles for certified reference samples. Two closely related grapevine rootstocks differing by as little as a single di-nucleotide repeat could be rapidly differentiated while there was high reproducibility of melting profiles for identical cultivars.</p> <p>Conclusion</p> <p>This novel microsatellite analysis method allows high sample throughput with greatly reduced time to results for varietal certification and is amenable to other microsatellite analyses.</p

A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars-0

for each rootstock were amplified in order to show the reproducibility of melting curves.<p><b>Copyright information:</b></p><p>Taken from "A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars"</p><p>http://www.plantmethods.com/content/4/1/8</p><p>Plant Methods 2008;4():8-8.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2396621.</p><p></p

A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars-1

Crosatellite locus VVMD32. Five individual extractions for 5C and SO4 show the differentiation of these two rootstocks<p><b>Copyright information:</b></p><p>Taken from "A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars"</p><p>http://www.plantmethods.com/content/4/1/8</p><p>Plant Methods 2008;4():8-8.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2396621.</p><p></p

A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars-6

for each rootstock were amplified in order to show the reproducibility of melting curves.<p><b>Copyright information:</b></p><p>Taken from "A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars"</p><p>http://www.plantmethods.com/content/4/1/8</p><p>Plant Methods 2008;4():8-8.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2396621.</p><p></p

A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars-4

(3) query Riesling 1/reference Riesling mixed sample (4) query Riesling 2 (5) query Riesling 2/reference Riesling mixed sample.<p><b>Copyright information:</b></p><p>Taken from "A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars"</p><p>http://www.plantmethods.com/content/4/1/8</p><p>Plant Methods 2008;4():8-8.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2396621.</p><p></p