Membranas electrohiladas de doble acción para tratamiento de agua

Número de publicación: 2 663 129 Número de solicitud: 201600852 51 Int. CI.: B01D 61/00 (2006.01) C02F 1/44 (2006.01)Membranas electrohiladas de doble acción para tratamiento de agua. La presente invención consiste en un procedimiento para la fabricación de membranas activas basadas en fibras submicrométricas que combinan una acción antimicrobiana con la capacidad de retención de contaminantes apolares en solución acuosa. Las membranas se producen mediante un procedimiento de electrohilado en disolución acuosa a partir de mezclas de un poliácido y un polialcohol solubles en agua que se estabilizan mediante un procedimiento de curado y se post-funcionalizan mediante la incorporación de dendrímeros con terminación amino mediante un procedimiento de injertado can ayuda de un agente de acoplamiento. La aplicación del material es la producción de membranas o componentes de membranas multicapa para tratamiento de agua con acción antimicrobiana y con capacidad para retener contaminantes apolares.Universidad de Almerí

Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba

Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems