A Potencial use of edible mushrooms Pleurotus ostreatoroseus Singer (Pleurotaceae) and Lentinus sajor-caju (Fr.) Fr. (Polyporaceae) in metal remediation processes.

Increased heavy metal pollution generated through anthropogenic activities into the environment required the need for environmental recovery strategies, such as mycoremediation. The aim of the present study was to assess the effect of iron (Fe) in the mycelial growth of Pleurotus ostreatoroseus Singer and Lentinus sajor-caju (Fr.) Fr. The growth response was evaluated on Potato Dextrose Agar medium with varying concentrations (0 ppm, 10 ppm, 20 ppm, 40 ppm and 80 ppm) of the iron metal. Ferrous sulfate heptahydrate (FeSO4.7H2O) were evaluated in this study. The daily mycelia growths were measured and compared. The biomass production was determined using the same plates of the evaluation of the growth of mycelia. Results revealed that the growth of mycelia of P. ostreatoroseus occurs slowly in medium containing different iron concentrations, but in relationship to L. sajor-caju was able to produce the largest mycelial dry mass. Overall this study suggests that P. ostreatosroseus can be used as promising option for removal of iron metal in bioremediation strategies

Determinação dos efeitos de dois suplementos alimentares em Drosophila melanogaster

Pleurotus spp. are considered extremely rich mushroom species from the nutritional pointof view, providing immune-enhancement effects when consumed. However, few mushroomshave been tested for their phenotypic and genotypic responses in animal modelsto ensure the proper dosage for their use. This study aimed to evaluate the effects of twomushroom species on the reproductive capacity of Drosophila melanogaster. Pleurotuscitrinopileatus Singer and Lentinus sajor-caju (Fr.) Fr. were provided separately, in specificconcentrations, as feed supplements to the fruit flies. The total numbers of developedlarvae, pupae, and adults were then measured for each treatment. Inter simple sequencerepeats marker analysis was carried out to infer genotypic changes in the mushroom-fedflies. Our results suggest that Pleurotus spp. can cause positive changes to the flies’ reproductivecapacity, since Pleurotus citrinopileatus in particular accelerated the life cycle andrevealed a higher genetic dissimilarity of the diet-supplemented flies.Keywords: fruitflies, reproductive fitness, edible mushrooms.Pleurotus spp. são consideradas espécies de cogumelo extremamente ricas do ponto de vista nutricional, proporcionando reforço imunológico quando consumidas. No entanto, poucos cogumelos foram testados quanto à sua interferência fenotípica e genotípica em modelos animais para garantir a dosagem adequada para seu uso. Este estudo teve como objetivo avaliar os efeitos de duas espécies de cogumelos sobre a capacidade reprodutiva de Drosophila melanogaster. Pleurotus citrinopileatus Singer e Lentinus sajor-caju (Fr.) Fr. foram fornecidos separadamente, em concentrações específicas, como suplementos alimentares às moscas da fruta. O número total de larvas, pupas e adultos desenvolvidos foram então avaliados para cada tratamento. Análises de marcadores moleculares do tipo Inter simple sequence repeats foram realizadas para inferir mudanças genotípicas nas moscas alimentadas com os cogumelos. Nossos resultados sugerem que Pleurotus spp. podem causar mudanças positivas na capacidade reprodutiva das moscas, uma vez que Pleurotus citrinopileatus, em particular, acelerou o ciclo de vida e revelou uma maior dissimilaridade genética das moscas suplementadas com esse fungo em sua dieta.Palavras-chave: moscas das frutas, estímulo reprodutivo, cogumelos comestíveis