33 research outputs found
Restored invasion of mouse MO4 cells into chick heart in vitro through mutual conditioning at reduced temperature.
No full textEffect of lipid-derivatives on invasion in vitro and on surface glycoproteins of three rodent cell types.
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Decreased fucose incorporation in cell surface carbohydrates is associated with inhibition of invasion.
No full textA heterodimer comprised of two bovine lactoferrin antimicrobial peptides exhibits powerful bactericidal activity against Burkholderia pseudomallei
No full textEffect of racemic 1-o-octadecyl-2-o-methylglycero-3-phosphocholine (ET-18-OCH3) on invasion in vitro and on N-linked surface glycosylation
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Membrane-active mechanism of LFchimera against Burkholderia pseudomallei and Burkholderia thailandensis
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Evaluation of salivary mucins in children with deciduous and mixed dentition: comparative analysis between high and low caries-risk groups
No full textCyclizing disulfide-rich peptides using sortase A
No full textSortase A (SrtA) is an enzyme obtained from Staphylococcus aureus that catalyzes site-specific transpeptidation of surface proteins to the bacterial cell membrane. SrtA recognizes an LPXTG amino acid motif and cleaves between the Thr and Gly to form a thioester-linked acyl–enzyme intermediate. The intermediate is resolved in the presence of a nucleophilic N-terminal polyglycine resulting in ligation of the acyl donor to the polyglycine acceptor. Here we describe the application of SrtA as a tool for the cyclization of disulfide-rich peptides. Reactions are typically tailored to each disulfide-rich peptide with optimal conditions producing yields of 40–50% cyclized peptide
