1,529 research outputs found

    Time-resolved broadband analysis of slow-light propagation and superluminal transmission of electromagnetic waves in three-dimensional photonic crystals

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    A time-resolved analysis of the amplitude and phase of THz pulses propagating through three-dimensional photonic crystals is presented. Single-cycle pulses of THz radiation allow measurements over a wide frequency range, spanning more than an octave below, at and above the bandgap of strongly dispersive photonic crystals. Transmission data provide evidence for slow group velocities at the photonic band edges and for superluminal transmission at frequencies in the gap. Our experimental results are in good agreement with finite-difference-time-domain simulations.Comment: 7 pages, 11 figure

    Effective gravity from a quantum gauge theory in Euclidean space-time

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    We consider a SO(d)SO(d) gauge theory in an Euclidean dd-dimensional space-time, which is known to be renormalizable to all orders in perturbation theory for 2d42\le{d}\le4. Then, with the help of a space-time representation of the gauge group, the gauge theory is mapped into a curved space-time with linear connection. Further, in that mapping the gauge field plays the role of the linear connection of the curved space-time and an effective metric tensor arises naturally from the mapping. The obtained action, being quadratic in the Riemann-Christoffel tensor, at a first sight, spoils a gravity interpretation of the model. Thus, we provide a sketch of a mechanism that breaks the SO(d)SO(d) color invariance and generates the Einstein-Hilbert term, as well as a cosmological constant term, allowing an interpretation of the model as a modified gravity in the Palatini formalism. In that sense, gravity can be visualized as an effective classical theory, originated from a well defined quantum gauge theory. We also show that, in the four dimensional case, two possibilities for particular solutions of the field equations are the de Sitter and Anti de Sitter space-times.Comment: 20 pages; Final version accepted for publication in Class.Quant.Gra

    Origin of replication of pBR345 plasmid DNA.

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    Aluminización de aceros ferriticos-martensiticos (hcm-12a) mediante cvd-fbr

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    Los recubrimientos de Aluminio son bien conocidos por tener una buena resistencia a la oxidación y a la sulfidización debido a la formación de una capa protectora de alúmina. La Deposición Química en Fase de Vapor mediante Lecho Fluidizado (CVD-FBR), debido a que es un proceso en el que se consiguen altas trasferencias de masa y calor a bajas temperaturas de operación. Además, tiene la ventaja de que se trabaja a presión atmosférica. La deposición de recubrimientos de Al sobre el acero ferrítico (HCM-12A) ha sido desarrollada mediante CVD-FBR. Los parámetros iniciales del proceso fueron determinados por la simulación termodinámica del proceso con la ayuda del programa informático “Thermocalc software”

    Genetic and biochemical analyses of chromosome and plasmid gene homologues encoding ICL and ArCP domains in Vibrioanguillarum strain 775

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    Anguibactin, the siderophore produced by Vibrio anguillarum 775 is synthesized from 2,3-dihydroxybenzoic acid (DHBA), cysteine and hydroxyhistamine via a nonribosomal peptide synthetase (NRPS) mechanism. Most of the genes encoding anguibactin biosynthetic proteins are harbored by the pJM1 plasmid. In this work we report the identification of a homologue of the plasmid-encoded angB on the chromosome of strain 775. The product of both genes harbor an isochorismate lyase (ICL) domain that converts isochorismic acid to 2,3-dihydro-2,3-dihydroxybenzoic acid, one of the steps of DHBA synthesis. We show in this work that both ICL domains are functional in the production of DHBA in V. anguillarum as well as in E. coli. Substitution by alanine of the aspartic acid residue in the active site of both ICL domains completely abolishes their isochorismate lyase activity in vivo. The two proteins also carry an aryl carrier protein (ArCP) domain. In contrast with the ICL domains only the plasmid encoded ArCP can participate in anguibactin production as determined by complementation analyses and site-directed mutagenesis in the active site of the plasmid encoded protein, S248A. The site-directed mutants, D37A in the ICL domain and S248A in the ArCP domain of the plasmid encoded AngB were also tested in vitro and clearly show the importance of each residue for the domain function and that each domain operates independently.

    Taxing the Informal Economy: The Current State of Knowledge and Agendas for Future Research

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    This paper reviews the literature on taxation of the informal economy, taking stock of key debates and drawing attention to recent innovations. Conventionally, the debate on whether to tax has frequently focused on the limited revenue potential, high cost of collection, and potentially adverse impact on small firms. Recent arguments have increasingly emphasised the more indirect benefits of informal taxation in relation to economic growth, broader tax compliance, and governance. More research is needed, we argue, into the relevant costs and benefits for all, including quasi-voluntary compliance, political and administrative incentives for reform, and citizen-state bargaining over taxation

    Operational Dynamic Modeling Transcending Quantum and Classical Mechanics

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    We introduce a general and systematic theoretical framework for Operational Dynamic Modeling (ODM) by combining a kinematic description of a model with the evolution of the dynamical average values. The kinematics includes the algebra of the observables and their defined averages. The evolution of the average values is drawn in the form of Ehrenfest-like theorems. We show that ODM is capable of encompassing wide ranging dynamics from classical non-relativistic mechanics to quantum field theory. The generality of ODM should provide a basis for formulating novel theories.Comment: 23 pages and 2 figures. Sec. VII B "Phase Space Representation in Curvilinear Coordinates" was correcte

    Fractional oscillator process with two indices

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    We introduce a new fractional oscillator process which can be obtained as solution of a stochastic differential equation with two fractional orders. Basic properties such as fractal dimension and short range dependence of the process are studied by considering the asymptotic properties of its covariance function. The fluctuation--dissipation relation of the process is investigated. The fractional oscillator process can be regarded as one-dimensional fractional Euclidean Klein-Gordon field, which can be obtained by applying the Parisi-Wu stochastic quantization method to a nonlocal Euclidean action. The Casimir energy associated with the fractional field at positive temperature is calculated by using the zeta function regularization technique.Comment: 32 page

    Stable Maintenance of Multiple Plasmids in E. coli Using a Single Selective Marker

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    Plasmid-based genetic systems in Escherichia coli are a staple of synthetic biology. However, the use of plasmids imposes limitations on the size of synthetic gene circuits and the ease with which they can be placed into bacterial hosts. For instance, unique selective markers must be used for each plasmid to ensure their maintenance in the host. These selective markers are most often genes encoding resistance to antibiotics such as ampicillin or kanamycin. However, the simultaneous use of multiple antibiotics to retain different plasmids can place undue stress on the host and increase the cost of growth media. To address this problem, we have developed a method for stably transforming three different plasmids in E. coli using a single antibiotic selective marker. To do this, we first examined two different systems with which two plasmids may be maintained. These systems make use of either T7 RNA polymerase-specific regulation of the resistance gene or split antibiotic resistance enzymes encoded on separate plasmids. Finally, we combined the two methods to create a system with which three plasmids can be transformed and stably maintained using a single selective marker. This work shows that large-scale plasmid-based synthetic gene circuits need not be limited by the use of multiple antibiotic resistance genes
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