Upregulated IL-32 expression and reduced gut short chain fatty acid caproic acid in people living with HIV with subclinical atherosclerosis

Despite the success of antiretroviral therapy (ART), people living with HIV (PLWH) are still at higher risk for cardiovascular diseases (CVDs) that are mediated by chronic inflammation. Identification of novel inflammatory mediators with the inherent potential to be used as CVD biomarkers and also as therapeutic targets is critically needed for better risk stratification and disease management in PLWH. Here, we investigated the expression and potential role of the multi-isoform proinflammatory cytokine IL-32 in subclinical atherosclerosis in PLWH (n=49 with subclinical atherosclerosis and n=30 without) and HIV- controls (n=25 with subclinical atherosclerosis and n=24 without). While expression of all tested IL-32 isoforms (α, β, γ, D, ϵ, and θ) was significantly higher in peripheral blood from PLWH compared to HIV- controls, IL-32D and IL-32θ isoforms were further upregulated in HIV+ individuals with coronary artery atherosclerosis compared to their counterparts without. Upregulation of these two isoforms was associated with increased plasma levels of IL-18 and IL-1β and downregulation of the atheroprotective protein TRAIL, which together composed a unique atherosclerotic inflammatory signature specific for PLWH compared to HIV- controls. Logistic regression analysis demonstrated that modulation of these inflammatory variables was independent of age, smoking, and statin treatment. Furthermore, our in vitro functional data linked IL-32 to macrophage activation and production of IL-18 and downregulation of TRAIL, a mechanism previously shown to be associated with impaired cholesterol metabolism and atherosclerosis. Finally, increased expression of IL-32 isoforms in PLWH with subclinical atherosclerosis was associated with altered gut microbiome (increased pathogenic bacteria; Rothia and Eggerthella species) and lower abundance of the gut metabolite short-chain fatty acid (SCFA) caproic acid, measured in fecal samples from the study participants. Importantly, caproic acid diminished the production of IL-32, IL-18, and IL-1β in human PBMCs in response to bacterial LPS stimulation. In conclusion, our studies identified an HIV-specific atherosclerotic inflammatory signature including specific IL-32 isoforms, which is regulated by the SCFA caproic acid and that may lead to new potential therapies to prevent CVD in ART-treated PLWH

The analysis of the influence factors affecting the performance of pharmaceutical companies

The paper provides an overview of current situation of the global pharmaceutical industry, with focus placed on the EU pharmaceutical market, including also the Romanian pharmaceutical market evolution synthetized into a SWOT analysis. As main goal the paper identifies several financial performance influence factors at the level of a number of companies from pharmaceutical sector, in order to generate a particular econometric model. The findings of the paper focus on the prediction of Return on Equity (ROE) ratio as the main performance measurement considered significant for the industry our and a large number of factors which contribute to its changes. Thereof, the paper investigates the relationship between various influence indicators or factors resulting from the financial situation of a company and the performance thereof and generates conclusions for the decisions makers at the level of the companies

Management Perspectives towards the Data-Driven Organization in the Energy Sector

This paper explores the current attitudes of managers and executives working in the energy sector towards the Data-Driven Organizational Model implied by Big Data. The aim is to explore and understand the current mindset of senior decision makers, since their success depends as much on cognitive and behavioral processes as on their technical competences. We adopt a grounded-theory approach, developing models of understanding and belief abductively, driven by the data obtained from participants through a reflection guide. We find that managers differ significantly in their understanding and engagement with their challenges; they display interest but differ in their commitment and enthusiasm; they identify a lack of strategy and skills as current barriers; and they are currently unwilling to trust data, treating evidence according to their own prior commitments. This is a significant barrier to establishing the Data-Driven Organizational Model. These findings raise concerns, and the paper concludes that by considering initiatives for implementing more agile and forward-looking approaches, establishing a data-driven organizational culture, and managing such changes effectively

Management Perspectives towards the Data-Driven Organization in the Energy Sector

This paper explores the current attitudes of managers and executives working in the energy sector towards the Data-Driven Organizational Model implied by Big Data. The aim is to explore and understand the current mindset of senior decision makers, since their success depends as much on cognitive and behavioral processes as on their technical competences. We adopt a grounded-theory approach, developing models of understanding and belief abductively, driven by the data obtained from participants through a reflection guide. We find that managers differ significantly in their understanding and engagement with their challenges; they display interest but differ in their commitment and enthusiasm; they identify a lack of strategy and skills as current barriers; and they are currently unwilling to trust data, treating evidence according to their own prior commitments. This is a significant barrier to establishing the Data-Driven Organizational Model. These findings raise concerns, and the paper concludes that by considering initiatives for implementing more agile and forward-looking approaches, establishing a data-driven organizational culture, and managing such changes effectively

Assessment of Soil Moisture Anomaly Sensitivity to Detect Drought Spatio-Temporal Variability in Romania

This paper will assess the sensitivity of soil moisture anomaly (SMA) obtained from the Soil water index (SWI) product Metop ASCAT, to identify drought in Romania. The SWI data were converted from relative values (%) to absolute values (m3 m−3) using the soil porosity method. The conversion results (SM) were validated using soil moisture in situ measurements from ISMN at 5 cm depths (2015–2020). The SMA was computed based on a 10 day SWI product, between 2007 and 2020. The analysis was performed for the depths of 5 cm (near surface), 40 cm (sub surface), and 100 cm (root zone). The standardized precipitation index (SPI), land surface temperature anomaly (LST anomaly), and normalized difference vegetation index anomaly (NDVI anomaly) were computed in order to compare the extent and intensity of drought events. The best correlations between SM and in situ measurements are for the stations located in the Getic Plateau (Bacles (r = 0.797) and Slatina (r = 0.672)), in the Western Plain (Oradea (r = 0.693)), and in the Moldavian Plateau (Iasi (r = 0.608)). The RMSE were between 0.05 and 0.184. Furthermore, the correlations between the SMA and SPI, the LST anomaly, and the NDVI anomaly were significantly registered in the second half of the warm season (July–September). Due to the predominantly agricultural use of the land, the results can be useful for the management of water resources and irrigation in regions frequently affected by drought

Yield of CT pulmonary angiography in the diagnosis of acute pulmonary embolism: short report

Abstract Objective To assess computed tomography pulmonary angiography (CTPA) positive yield rate for pulmonary embolism (PE) in a Canadian academic tertiary center. Results This one-center retrospective cross-sectional study includes from 5565 (model 1) to 5296 (model 2) patients that were evaluated for suspected PE in 2015, among which 1331 (23.9% (model 1) to 25.1% (model 2)) underwent CTPA. Mean age of CTPA patients was 60.2 ± 16.6 years, of which 575 were males (43.2%). Two hundred eleven CTPA examinations were positive for PE, giving a CTPA positive yield rate of 15.9% (95% CI (13.93–17.87)). One hundred and thirteen (8.1%) CTPA were considered indeterminate, and eleven were considered nondiagnostic (0.8%). Among the 211 CTPA positive for PE, 67 (32%) were proximal emboli, 98 (47%) were segmental emboli and 44 (21%%) were subsegmental emboli. In conclusion, in this retrospective study done in a Canadian academic tertiary center, we report a positive rate of 15.9% for PE detection with CTPA, which is above the generally accepted lower threshold of 10% for the yield of CTPA