Health and Health-related Problems in a Cohort of Apprentices in Switzerland

Out of a cohort of 1200 apprentices in Switzerland, 781 boys and 417 girls completed a questionnaire on their perceived health and health problems in their selected apprenticeship. Each participant was interviewed afterwards by the school nurses in three state-owned professional schools where the apprentices attended their classes. Ninety-five per cent of the respondents enjoyed excellent health, 28 cases had nervous system or psychological problems, 17 cases had problems of a locomotor nature, 12 subjects had gastrointestinal or endocrinological symptoms, and there was one with a past history of a malignant disease. Twelve cases also had respiratory symptoms or diagnoses. One hundred and nineteen apprentices reported that they had been troubled by the workplace. Exposure to solvents, chemicals in general, dust, smoke and noise were often mentioned as causes. Thirty-eight of them had contacted their family physician in connection with these problems. The cohort had experienced, mostly during their first year, 191 cases of accident necessitating medical care. Cuts, shocks and falls were the most common occurrences. Meat cutters and butchers had the highest frequency of accidents. Near-accidents had been experienced so far by 46 per cent of the respondents. At the interviews, school nurses provided counsel and intervened in flagrant cases of occupational risks, It seems that an ordinary medical certificate does not predict accident-proneness or possible medical problems, while in-school health programmes could provide rapid intervention and suppor

Calcium entry via TRPV1 but not ASICs induces neuropeptide release from sensory neurons

Inflammatory mediators induce neuropeptide release from nociceptive nerve endings and cell bodies, causing increased local blood flow and vascular leakage resulting in edema. Neuropeptide release from sensory neurons depends on an increase in intracellular Ca2+ concentration. In this study we investigated the role of two types of pH sensors in acid-induced Ca2+ entry and neuropeptide release from dorsal root ganglion (DRG) neurons. The transient receptor potential vanilloid 1 channel (TRPV1) and acid-sensing ion channels (ASICs) are both H+-activated ion channels present in these neurons, and are therefore potential pH sensors for this process. We demonstrate with in situ hybridization and immunocytochemistry that TRPV1 and several ASIC subunits are co-expressed with neuropeptides in DRG neurons. Activation of ASICs and of TRPV1 led to an increase in intracellular Ca2+ concentration. While TRPV1 has a high Ca2+ permeability and allows direct Ca2+ entry when activated, we show here that ASICs of DRG neurons mediate Ca2+ entry mostly by depolarization-induced activation of voltage-gated Ca2+ channels and only to a small extent via the pore of Ca2+-permeable ASICs. Extracellular acidification led to release of the neuropeptide calcitonin gene-related peptide from DRG neurons. The pH dependence and the pharmacological profile indicated that TRPV1, but not ASICs, induced neuropeptide secretion. In conclusion, this study shows that although both TRPV1 and ASICs mediate Ca2+ influx, TRPV1 is the principal sensor for acid-induced neuropeptide secretion from sensory neurons

Hydropeaking indicators for characterization of the Upper-Rhone River in Switzerland

River channelization and the construction of high-head storage schemes have been the basis of agricultural and socio-economic development in many alpine regions. One example is the Upper-Rhone River in Switzerland. The Upper-Rhone's morphology changed considerably between 1863 and 1960 as a result of two major channelizations and, from 1950 on, the construction of a large number of high-head storage hydropower schemes in the catchment. These modifications have brought large benefits to the local population, at the cost, however, of substantial disturbances in aquatic and terrestrial ecosystems in and along the river. A primary factor behind these disturbances is the alteration of the natural flow regime, namely hydropeaking due to the operation of the high-head storage hydropower plants. For sustainable river-restoration projects on regulated rivers, scientists and engineers now widely accept the necessity of integrated management of the river. Different aspects such as river morphology, sediment management, water quality, temperature, and the naturally variable flow regime should be considered simultaneously. Mitigation of non-natural, sub-daily flow fluctuations due to hydropeaking is a crucial step in restoring natural flow regimes, but is especially challenging due to the economic constraints such mitigation places upon hydropower plants. With the goal of addressing this challenge, this paper proposes three indicators to describe the flow regime of rivers in alpine catchments with and without high-head storage hydropower plants. The indicators quantify: (1) the seasonal distribution and transfer of water, (2) sub-daily flow fluctuations, and (3) the intensity and frequency of flow changes. Indicators are evaluated in a case study of the Upper-Rhone River for pre- and post-impact situations, and the benefit of a multipurpose project reducing hydropeaking on hydrologic conditions is quantified. Furthermore, the paper explores the possibility of using these indicators to link aquatic and terrestrial ecosystem well being to their hydrolog

On the Mathematical and Geometrical Structure of the Determining Equations for Shear Waves in Nonlinear Isotropic Incompressible Elastodynamics

Using the theory of $1+1$ hyperbolic systems we put in perspective the mathematical and geometrical structure of the celebrated circularly polarized waves solutions for isotropic hyperelastic materials determined by Carroll in Acta Mechanica 3 (1967) 167--181. We show that a natural generalization of this class of solutions yields an infinite family of \emph{linear} solutions for the equations of isotropic elastodynamics. Moreover, we determine a huge class of hyperbolic partial differential equations having the same property of the shear wave system. Restricting the attention to the usual first order asymptotic approximation of the equations determining transverse waves we provide the complete integration of this system using generalized symmetries.Comment: 19 page

ROLES OF ACID-SENSING ION CHANNELS (ASICS) IN PERIPHERAL NEUROPEPTIDE SECRETION AND PAIN

Acid-sensing ion channels (ASICs) are non-voltage-gated sodium channels activated by an extracellular acidification. They are widely expressed in neurons of the central and peripheral nervous system. ASICs have a role in learning, the expression of fear, in neuronal death after cerebral ischemia, and in pain sensation. Tissue damage leads to the release of inflammatory mediators. There is a subpopulation of sensory neurons which are able to release the neuropeptides calcitonin gene-related peptide (CGRP) and substance P (SP). Neurogenic inflammation refers to the process whereby peripheral release of the neuropeptides CGRP and SP induces vasodilation and extravasation of plasma proteins, respectively. Our laboratory has previously shown that calcium-permeable homomeric ASIC1a channels are present in a majority of CGRP- or SP-expressing small diameter sensory neurons. In the first part of my thesis, we tested the hypothesis that a local acidification can produce an ASIC-mediated calcium-dependant neuropeptide secretion. We have first verified the co-expression of ASICs and CGRP/SP using immunochemistry and in-situ hybridization on dissociated rat dorsal root ganglion (DRG) neurons. We found that most CGRP/SP-positive neurons also expressed ASIC1a and ASIC3 subunits. Calcium imaging experiments with Fura-2 dye showed that an extracellular acidification can induce an increase of intracellular Ca2+ concentration, which is essential for secretion. This increase of intracellular Ca2+ concentration is, at least in some cells, ASIC-dependent, as it can be prevented by amiloride, an ASIC antagonist, and by Psalmotoxin (PcTx1), a specific ASIC1a antagonist. We identified a sub-population of neurons whose acid-induced Ca2+ entry was completely abolished by amiloride, an amiloride-resistant population which does not express ASICs, but rather another acid-sensing channel, possibly transient receptor potential vanilloïde 1 (TRPV1), and a population expressing both H+-gated channel types. Voltage-gated calcium channels (Cavs) may also mediate Ca2+ entry. Co-application of the Cavs inhibitors (ω-conotoxin MVIIC, Mibefradil and Nifedipine) reduced the Ca2+ increase in neurons expressing ASICs during an acidification to pH 6. This indicates that ASICs can depolarise the neuron and activate Cavs. Homomeric ASIC1a are Ca2+-permeable and allow a direct entry of Ca2+ into the cell; other ASICs mediate an indirect entry of Ca2+ by inducing a membrane depolarisation that activates Cavs. We showed with a secretion assay that CGRP secretion can be induced by extracellular acidification in cultured rat DRG neurons. Amiloride and PcTx1 were not able to inhibit the secretion at acidic pH, but BCTC, a TRPV1 inhibitor was able to decrease the secretion induced by an extracellular acidification in our in vitro secretion assay. In conclusion, these results show that in DRG neurons a mild extracellular acidification can induce a calcium-dependent neuropeptide secretion. Even if our data show that ASICs can mediate an increase of intracellular Ca2+ concentration, this appears not to be sufficient to trigger neuropeptide secretion. TRPV1, a calcium channel whose activation induces a sustained current - in contrary of ASICs - played in our experimental conditions a predominant role in neurosecretion. In the second part of my thesis, we focused on the role of ASICs in neuropathic pain. We used the spared nerve injury (SNI) model which consists in a nerve injury that induces symptoms of neuropathic pain such as mechanical allodynia. We have previously shown that the SNI model modifies ASIC currents in dissociated rat DRG neurons. We hypothesized that ASICs could play a role in the development of mechanical allodynia. The SNI model was performed on ASIC1a, -2, and -3 knock-out mice and wild type littermates. We measured mechanical allodynia on these mice with calibrated von Frey filaments. There were no differences between the wild-type and the ASIC1, or ASIC2 knockout mice. ASIC3 null mice were less sensitive than wild type mice at 21 day after SNI, indicating a role for ASIC3. Finally, to investigate other possible roles of ASICs in the perception of the environment, we measured the baseline heat responses. We used two different models; the tail flick model and the hot plate model. ASIC1a null mice showed increased thermal allodynia behaviour in the hot plate test at three different temperatures (49, 52, 55°C) compared to their wild type littermates. On the contrary, ASIC2 null mice showed reduced thermal allodynia behaviour in the hot plate test compared to their wild type littermates at the three same temperatures. We conclude that ASIC1a and ASIC2 in mice can play a role in temperature sensing. It is currently not understood how ASICs are involved in temperature sensing and what the reason for the opposed effects in the two knockout models is

Born-Infeld Theory and Stringy Causality

Fluctuations around a non-trivial solution of Born-Infeld theory have a limiting speed given not by the Einstein metric but the Boillat metric. The Boillat metric is S-duality invariant and conformal to the open string metric. It also governs the propagation of scalars and spinors in Born-Infeld theory. We discuss the potential clash between causality determined by the closed string and open string light cones and find that the latter never lie outside the former. Both cones touch along the principal null directions of the background Born-Infeld field. We consider black hole solutions in situations in which the distinction between bulk and brane is not sharp such as space filling branes and find that the location of the event horizon and the thermodynamic properties do not depend on whether one uses the closed or open string metric. Analogous statements hold in the more general context of non-linear electrodynamics or effective quantum-corrected metrics. We show how Born-Infeld action to second order might be obtained from higher-curvature gravity in Kaluza-Klein theory. Finally we point out some intriguing analogies with Einstein-Schr\"odinger theory.Comment: 31 pages, 4 figures, LaTex; Some comments and references adde

Halloween de John Carpenter: une architecture du regard.

Analyse de la représentation d l'espace dans le film Halloween de John Carpenter (1958)