Gender Specific Emotional Responses to Anticipated Crime

In this paper multivariate analyses are used to test two hypotheses specific to the assumption that women are more fearful of crime than men. First, national crime survey responses to a global fear of crime question were analysed to assess whether responses to global questions were biased towards particular crime types. Results show that non-specific global fear of crime questions elicit responses most associated with fear of physical harm - explaining the persistent finding in previous research that women are more fearful than men. Second, a two-dimensional measure of fear of crime was derived from six crime specific fear of crime responses. Gender and control variables were regressed onto the derived measures of fear to test the hypothesis that dimensions of fear are gender specific. Results show that women are relatively more fearful of personal harm but no gender difference was found for fear of property loss. These data are consistent with a physical vulnerability explanation and, taken together, suggest that the irrationality hypothesis can be rejected

Methods for Isolating, Identifying, and Quantifying Anthocyanin Metabolites in Clinical Samples

The metabolic fate of anthocyanins until recently was relatively unknown, primarily as a result of their instability at physiological pH and a lack of published methods for isolating and identifying their metabolites from biological samples. The aim of the present work was to establish methods for the extraction and quantification of anthocyanin metabolites present in urine, serum, and fecal samples. 35 commercial and 10 synthetic analytes, including both known and predicted human and microbial metabolites of anthocyanins, were obtained as reference standards. HPLC and MS/MS conditions were optimized for organic modifier, ionic modifier, mobile phase gradient, flow rate, column type, MS source, and compound dependent parameters. The impact of sorbent, solvent, acid, preservative, elution, and evaporation on solid phase extraction (SPE) efficiency was also explored. The HPLC-MS/MS method validation demonstrated acceptable linearity (R(2), 0.997 +/- 0.002) and sensitivity (limits of detection (LODs): urine, 100 +/- 375 nM; serum, 104 +/- 358 nM; feces 138 +/- 344 nM), and the final SPE methods provided recoveries of 88.3 +/- 17.8% for urine, 86.5 +/- 11.1% for serum, and 80.6 +/- 20.9% for feces. The final methods were applied to clinical samples derived from an anthocyanin intervention study, where 36 of the 45 modeled metabolites were detected within urine, plasma, or fecal samples. The described methods provide suitable versatility for the identification and quantification of an extensive series of anthocyanin metabolites for use in future clinical studies exploring absorption, distribution, metabolism, and elimination.</p

Methods for Isolating, Identifying, and Quantifying Anthocyanin Metabolites in Clinical Samples

The metabolic fate of anthocyanins until recently was relatively unknown, primarily as a result of their instability at physiological pH and a lack of published methods for isolating and identifying their metabolites from biological samples. The aim of the present work was to establish methods for the extraction and quantification of anthocyanin metabolites present in urine, serum, and fecal samples. 35 commercial and 10 synthetic analytes, including both known and predicted human and microbial metabolites of anthocyanins, were obtained as reference standards. HPLC and MS/MS conditions were optimized for organic modifier, ionic modifier, mobile phase gradient, flow rate, column type, MS source, and compound dependent parameters. The impact of sorbent, solvent, acid, preservative, elution, and evaporation on solid phase extraction (SPE) efficiency was also explored. The HPLC-MS/MS method validation demonstrated acceptable linearity (R(2), 0.997 +/- 0.002) and sensitivity (limits of detection (LODs): urine, 100 +/- 375 nM; serum, 104 +/- 358 nM; feces 138 +/- 344 nM), and the final SPE methods provided recoveries of 88.3 +/- 17.8% for urine, 86.5 +/- 11.1% for serum, and 80.6 +/- 20.9% for feces. The final methods were applied to clinical samples derived from an anthocyanin intervention study, where 36 of the 45 modeled metabolites were detected within urine, plasma, or fecal samples. The described methods provide suitable versatility for the identification and quantification of an extensive series of anthocyanin metabolites for use in future clinical studies exploring absorption, distribution, metabolism, and elimination.</p