Évaluation des risques professionnels dans un théâtre de spectacles vivants

International audienc

[Characterization of gonadotropic cells in a new pituitary tumor cell line]

The pituitary tumor cell line RC-4B/C was established in The Jackson Laboratory from an aged rat pituitary adenoma. Immunocytochemical studies of this cell line showed that all pituitary cell types were present. Approximately 20% reacted with antisera (AS) to ovine (o) LH beta, 8.6% with AS to oFSH beta, 15% with AS to rat PRL, 12% with AS to equine GH, 9% with AS to porcine TSH beta and 8.6% with AS to ACTH1-24. Using NIDDK rat kits, RIA showed about 0.38, 0.08 and 607.50 ng per 10(6) cells of LH, FSH and PRL, respectively, vs 33.9, 75.6 and 573 ng in freshly dispersed rat pituitary cells. The GnRH receptor content of the cell line was about a half that of normal rat pituitary cells but the receptor affinity was the same. A chronic treatment of the cells for about 5 months with a sub-physiological: concentration (3.7 pM) of a GnRH agonist had 3 major effects: 1) as compared to the controls, a 3-fold increase in the cell number in the log phase; 2) an increase of the percentage of FSH beta cells from 8.6 to 21.9% whereas LH beta cells and the cell content of LH and FSH remained stationary; 3) a decrease of the percentage of PRL cells from 15 to 6.5% and an almost 250-fold decrease of PRL cell content. Incorporation studies with [35S] Met demonstrated that the alpha subunit in the cell line was only partly glycosylated. Pretreatment of the cells with 5 nM estradiol restored, at least partly, glycosylation of alpha.(ABSTRACT TRUNCATED AT 250 WORDS

Objectivation des modifications cutanées cortico-induites en fonction de l'âge

Hors-Série 3 - Journées dermatologiques de Paris 2012National audienc

Non-invasive short-term assessment of retinoids effects on human skin in vivo using multiphoton microscopy

International audienceBackgroundThe occlusive patch test developed for assessing topical retinoids activity in human skin has been extended as a short-term screening protocol for anti-ageing agents. In this model, biopsies are performed at the end of the occlusion period for morphological and immuno-histochemistry analysis. Multiphoton microscopy is a recent non-invasive imaging technique that combined with image processing tools allows the in vivo quantification of human skin modifications.ObjectiveTo validate with gold standards of anti-ageing that are retinoids, the relevance of multiphoton microscopy for kinetic and quantitative assessment in this model.MethodsTwenty women, aged 50–65 years, were enrolled. Retinol 0.3% (RO) and Retinoic acid 0.025% (RA) were applied to the dorsal photo-damaged side of their forearm under occlusive patches for 12 days. A patch alone was applied to a third area as control. Evaluation was performed at day D0, D12 (end of treatment), D18 and D32 using multiphoton microscopy. Epidermal thickness, normalized area of the dermal-epidermal junction (DEJ) and melanin density were estimated using 3D image processing tools.ResultsMain significant results are: Epidermal thickening at D12, D18 and D32 with RO and at D12, D18 with RA vs. baseline and vs. control. Increased DEJ undulation at D32 with RO and at D12 with RA vs. baseline and vs. control. Decreased melanin content with RO (at D12 and D18 vs. baseline and at D32 vs. baseline and vs. control) and with RA (at D12 vs. baseline).ConclusionsThis study shows that multiphoton microscopy associated to specific 3D image processing tools allows cutaneous effects induced by topical retinoids in this in vivo model to be non-invasively detected, quantified and followed over time. This innovative approach could be applied to the evaluation of other active compounds

A time- and single-cell-resolved model of murine bone marrow hematopoiesis.

The paradigmatic hematopoietic tree model is increasingly recognized to be limited, as it is based on heterogeneous populations largely defined by non-homeostatic assays testing cell fate potentials. Here, we combine persistent labeling with time-series single-cell RNA sequencing to build a real-time, quantitative model of in vivo tissue dynamics for murine bone marrow hematopoiesis. We couple cascading single-cell expression patterns with dynamic changes in differentiation and growth speeds. The resulting explicit linkage between molecular states and cellular behavior reveals widely varying self-renewal and differentiation properties across distinct lineages. Transplanted stem cells show strong acceleration of differentiation at specific stages of erythroid and neutrophil production, illustrating how the model can quantify the impact of perturbations. Our reconstruction of dynamic behavior from snapshot measurements is akin to how a kinetoscope allows sequential images to merge into a movie. We posit that this approach is generally applicable to understanding tissue-scale dynamics at high resolution

Risk factors for relapse in patients with bullous pemphigoid in clinical remission: a multicenter, prospective, cohort study.

International audienc