Tuberculose extrapulmonar em pacientes com AIDS na cidade do Rio Grande, Brasil. Diagnóstico laboratorial

ABSTRACTOne hundred two specimens of extrapulmonary material from 74 AIDS patients from the AIDS Service of University Hospital, FURG, Rio Grande, RS, Brazil, were examined at Mycobacteria Laboratory, between September 1997 and December 1999. This study had the aim to evaluate laboratoy importance in the diagnosis of extrapulmonary tuberculosis in AIDS patients with clinical suggestive symptoms of tuberculosis. Acid-fast bacilli (AFB) stains were performed using the Kinyon Method (cold stainning). Mycobacterial isolation was made In the Ogawa medium. The Ogawa medium added with p-nitrobenzoic acid, 2-tiophenocarboxylic acid hidrazide and pyruvic acid sodium salt was used to the Identification of mycobacterial species. Confirmation of Mycobacterium tuberculosis was done by the niacin test. The extrapulmonary tuberculosis diagnosis was done in 12,74% of specimens from 17,56% of the patients. Distribution of specimens with positive results in relation to the obtained sites were: 8/13 lymph node biopsies, 2/13 liver biopsies, 2/13 ascitic nuid and 1/13 pleural nuid. ABF were found on microscopic examination from 7/13 specimens and 10/13 in culture. The microscopic examination of ABF stain was the only method responsible for the extrapulmonary tuberculosis diagnostic in 3/13 cases and the culture in 6/13 cases. Among the positive specimens at microscopic examination or ABF stains, 6/7 were lymph node biopsies and In ascitic fluid. The used methods in the diagnosis, of the extrapulmonary tuberculosis showed good results, by this way we strongly recommend the realization of both as routine in our place.REV PORT PNEUMOL 2000; VI (4): 277-28

Status of COLDDIAG: A Cold Vacuum Chamber for Diagnostics

One of the still open issues for the development of superconducting insertion devices is the understanding of the beam heat load. With the aim of measuring the beam heat load to a cold bore and the hope to gain a deeper understanding in the beam heat load mechanisms, a cold vacuum chamber for diagnostics is under construction. The following diagnostics will be implemented: i) retarding field analyzers to measure the electron energy and flux, ii) temperature sensors to measure the total heat load, iii) pressure gauges, iv) and mass spectrometers to measure the gas content. The inner vacuum chamber will be removable in order to test different geometries and materials. This will allow the installation of the cryostat in different synchrotron light sources. COLDDIAG will be built to fit in a short straight section at ANKA. A first installation at the synchrotron light source Diamond is foreseen in June 2011. Here we describe the technical design report of this device and the planned measurements with beam.Comment: Presented at First International Particle Accelerator Conference, IPAC'10, Kyoto, Japan, from 23 to 28 May 201

Effect of rMnSOD on sodium reabsorption in renal proximal tubule in Ochratoxin A - treated rats

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium that represent toxic real threat for human beings and animal health. In this study we evaluated the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) on oxidative stress and on the alterations of fluid reabsorption in renal proximal tubule (PT) as possible causes of OTA nephrotoxicity. Finally, we have measured the concentration of O2 - in the kidney through dihydroethidium assay (DHE) and nitric oxide (NO) concentration through nitrites and nitrates assay. Male Sprague Dawley rats weighing 120-150 g were treated for 14 days by gavage, as follows: Control group, 12 rats received a corresponding amount of saline solution (including 10% DMSO); rMnSOD group, 12 rats treated with rMnSOD (10 μg/kg bw); OTA group, 12 rats treated with OTA (0,5 mg/Kg bw) dissolved in 10% DMSO and then scaled to required volume with corn oil; rMnSOD + OTA, 12 rats treated with rMnSOD (10 μg /kg bw) plus OTA (0,5 mg/Kg bw). Our results have shown that rMnSOD restores the alteration of reabsorption in PT in rats treated with OTA plus rMnSOD, probably through the response to pressure natriuresis, where nitric oxide plays a key role. Moreover, rMnSOD prevents the nephrotoxicity induced by OTA probably restoring the balance between superoxide and NO that is most probably the cause of hypertension and renal functional alterations through the inhibition of NO synthase. In conclusion these data provide important information for understanding of mechanism of toxic action of OTA

Beam physics research with the IOTA electron lens

The electron lens in the Fermilab Integrable Optics Test Accelerator (IOTA) will enable new research in nonlinear integrable optics, space-charge compensation, electron cooling, and the stability of intense beams. This research addresses scientific questions on high-brightness beams and operational challenges of high-power accelerators for nuclear and particle physics. We review the roles that electron lenses play in this field and the physical principles behind their applications. The design criteria and specifications for the IOTA storage ring and electron lens are then discussed. We conclude with a description of the components of the apparatus