Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty

<p>Abstract</p> <p>Background</p> <p>Post-puberty deterioration of kidneys is more rapid in males than in females. To reveal the underlying molecular mechanisms for this difference, we analyzed gender-dependent gene expression in kidneys of three groups of 36 day-old rats.</p> <p>Results</p> <p>The number of genes exhibiting gender-dependent expression was highly influenced by the genetic background of the rat group examined. 373, 288 and 79 genes showed differential gene expression between males and females (p = 0.001) in US, Mhm and Mhm*BN rats, respectively. Of all gender dependently expressed genes, only 39 genes were differentially expressed in all tested groups and the direction of expression change was the same for those genes for all groups. The gene expression profile suggests higher metabolic and transport activities, enhanced cell proliferation, elevated oxidative stress, and altered vascular biology in males. Furthermore, elevated levels of superoxide anion (two- to three-fold) in males compared to females were detected at early puberty, but neither at pre-puberty nor at late puberty/early adulthood.</p> <p>Conclusion</p> <p>Our data suggest that early puberty, with gender-related elevation in oxidative stress in males, is a key compromising factor on kidneys in males.</p

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-4

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>f ROS in males is shown on the y-axis (expressed as the ratio of male vs. female). In PBS-perfused kidneys, the elevation of superoxide anion in males was statistically significant. This statistical significance diminished when kidneys were flushed with heparin. Also the elevation of total ROS in males attenuated (the ratio of male vs. female became smaller). B: Real-time PCR confirmed significant under-expression of Sod3 in males compared with females (p < 0.01)

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-5

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>diagram the number of genes with gender-dependent expression (p = 0.001) in the respective groups is depicted

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-3

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>en genders. At pre-puberty and at late puberty/early adulthood, superoxide anion did not differ significantly between genders. Superoxide anion peaked at early puberty, more abruptly in males than in females (p = 0.05), and dropped to pre-puberty levels as late puberty/early adulthood approached

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-1

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>ith strongest expression changes between genders, the microarray data were confirmed by real-time PCR in Mhm rats

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-0

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>diagram the number of genes with gender-dependent expression (p = 0.001) in the respective groups is depicted

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-2

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>les had the tendency to produce more total ROS, but the gender difference was not statistically significant