Impact of washing-medium temperature on viability (A) and PBMC recovery (B).

<p>20 donors included. Ref.: reference group. **: p<0.01.</p

PBMC viability (A) and absolute live PBMC recovery (B) by incubation.

<p>Samples were stored in a 37°C incubator with 5% CO₂. Paired samples from 20 donors were included. Ref.: reference group. NS: non-significant. *: p<0.05. **: p<0.01.</p

Comparing two ways of mixing PBMCs and washing medium by viability (A) and absolute count of live PBMCs (B).

<p>Paired samples 20 donors included. Ref.: reference group. NS: non-significant. **: p<0.01.</p

Gating strategy to identify live/dead CD45+ leukocytes.

<p>A) Events were triggered on FSC-H at a deliberately low threshold to avoid accidental exclusion of dead cells. B) Doublet exclusion C) Identification of CD45 positive cells D) Dead cells identified as 7-AAD positive events. Compensation for spectral overlap was not required.</p

Temperature of centrifuge by PBMC viability (A) and absolute live PBMC recovery (B).

<p>Paired samples from 20 donors included. Ref.: reference group. NS: non-significant.</p

PBMC viability (A) and absolute recovery (B) as a result of varying thawing time in 37°C heated water bath.

<p>Paired samples from 20 donors included. Ref.: reference group. NS: non-significant.</p

Centrifugation time and force and PBMC viability (A) and absolute live PBMC count (B).

<p>Paired samples from 18 donors included. Ref.: reference group. NS: non-significant. *: p<0.05. **: p<0.01.</p

Characteristics of 4 patients with mutations in the HBV polymerase gene.

<p>Characteristics of 4 patients with mutations in the HBV polymerase gene.</p

Sociodemographic and clinical characteristics of pregnant women.

<p>Sociodemographic and clinical characteristics of pregnant women.</p

Crude and adjusted prevalence ratios for not testing for HIV among pregnant women.

<p>Crude and adjusted prevalence ratios for not testing for HIV among pregnant women.</p