Mapping epitopes onto the cis TPO dimer.

<p><b>(A)</b> Epitopes and residues identified in various studies as constituting immunodominant regions IDR-A (blue) and IDR-B (pink); <b>(B)</b> Lumen-facing side of MPO-like domain; <b>(C)</b> Close-up shows that much of the two immunodominant regions are on the lumen-facing surface of the MPO-like domain near the dimer interface, particularly IDR-B; <b>(D)</b> Membrane-facing side of TPO containing the heme (orange) cavity.</p

A comparison between membrane-embedded, dimeric models of TPO isoform1 in two different conformations.

<p><b>(A)</b> Schematic of trans model with active site facing away from follicular membrane. Coloring as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142615#pone.0142615.g001" target="_blank">Fig 1</a>; <b>(B)</b> Cis model with active site facing towards follicular membrane; <b>(C)</b> perpendicular views of trans model; <b>(D)</b> perpendicular views of cis model. TPO represented as cartoon and space filling (subunits A and B, respectively) for clarity. Domains are coloured as follows: MPO-like domain (green), CCP-like domain (Shamrock green), EGF-like domain (cyan-blue), and TM domain (dark-blue). Catalytic heme represented as red spheres, and DMPC molecules represented as lines and coloured as CPK.</p