Electro-oxidative depolymerisation of technical lignin in water using platinum, nickel oxide hydroxide and graphite electrodes

In order to improve the lignin exploitation to added-value bioproducts, a mild chemical conversion route based on electrochemistry was investigated. For the first time, soda lignin Protobind™ 1000 (technical lignin from the pulp & paper industry) was studied by cyclic voltammetry to preliminarily investigate the effect of the main reaction parameters, such as the type of electrode material (platinum, nickel oxide hydroxide, graphite), the pH (12, 13, 14), the scan rate (10, 50, 100, 250 mV s-1), the substrate concentration (2, 20 g L-1) and the oxidation/reduction potential (from -0.8 to +0.8 V). Under the optimal reaction conditions among those tested (NiOOH electrode, pH 14, lignin 20 g L-1, 0.4 V), the electro-oxidative depolymerisation of lignin by electrolysis was performed in a divided cell. The reaction products were identified and quantified by ultra-pressure liquid chromatography coupled with mass spectrometry. The main products were sinapic acid, vanillin, vanillic acid, and acetovanillone. The obtained preliminary results demonstrated the potential feasibility of this innovative electrochemical route for lignin valorisation for the production of bio-aromatic chemicals

Forced activation of Cdk1 via wee1 inhibition impairs homologous recombination

<p>In response to DNA breaks, the 'DNA damage response' provokes a cell cycle arrest to facilitate DNA repair. Recent findings have indicated that cells can respond to DNA damage throughout the cell cycle, except during mitosis. Specifically, various mitotic kinases, including Cdk1, Aurora A and Plk1, were shown to inactivate key DNA damage checkpoint proteins when cells enter mitosis. Aberrant activation of mitotic kinases during interphase could therefore modulate cellular responses to DNA damage. In this study, our aim was to determine how aberrant activation of Cdk1 affects the cellular responses to DNA damage. We used Wee1 inhibition, using MK-1775, to force Cdk1 activation, which did not cause cytotoxicity in non-transformed cells. Instead, it accelerated mitotic entry and caused radio sensitization in p53-defective cancer cells, but not in p53-proficient cancer cells. Interestingly, we showed that Wee1 inhibition leads to elevation of Cdk1 activity in interphase cells. When we subsequently analyzed DNA damage responses in cells with forced Cdk1 activation, we observed a marked reduction of 53BP1 at sites of DNA damage along with an increase in g-H2AX staining after irradiation, indicative of defective DNA repair. Indeed, when DNA repair was analyzed using in vivo endonuclease-induced homologous recombination (HR) assays, compromised DNA repair after Wee1 inhibition was confirmed. This defect in HR was accompanied by increased phosphorylation of BRCA2 at the Cdk1 phosphorylation site S3291. Taken together, our results indicate that Wee1 inhibition leads to forced Cdk1 activation in interphase cells, which interferes with normal DNA damage responses.</p>

Initializing a regional climate model with satellite-derived soil moisture

Regional climate simulations over Europe were initialized with soil moisture derived from the Advanced Microwave Scanning Radiometer-Earth Observing System (AMSR-E) in order to assess the model accuracy in predicting soil moisture and other components of the hydrological cycle. The AMSR-E soil moisture initially showed systematic differences with model-predicted soil moisture. For proper initialization the AMSR-E product had to be rescaled and after that vertically profiled. To retrieve a root zone soil moisture profile, we tested the application of an exponential filter. The smoothing through the layers of the ERA-Interim soil moisture profile was applied to the rescaled AMSR-E surface soil moisture. The filter performed very well for that part of the data set where the top layer is positively correlated with the deeper layers. After the preparation of the soil moisture fields, several sensitivity simulations were performed. The model's soil moisture was replaced with the vertically profiled AMSR-E soil moisture at different initial times for a dry summer (2003) and a wet summer (2005). In general, the surplus of soil moisture in the AMSR-E data resulted in a better performance in predicting temperature when compared with observations. This finding was more pronounced in the dry summer of 2003 when the model results appeared very sensitive to land-atmosphere feedbacks. Our results suggest that in dry years, the use of appropriate observed soil moisture may help more to reduce modeled surface temperatures than inducing additional rainfall in the model. Using the AMSR-E product led to a decrease in areal extent sensitive to land-atmosphere interactions. Copyright 2011 by the American Geophysical Union

What are cascading disasters?

Cascades have emerged as a new paradigm in disaster studies. The high level of dependency of modern populations on critical infrastructure and networks allows the impact of disasters to propagate through socio-economic systems. Where vulnerabilities overlap and interact, escalation points are created that can create secondary effects with greater impact than the primary event. This article explains how complexity can be categorised and analysed in order to find those weak points in society that enable cascading impacts to develop. Scenarios can be used to identify critical dependencies and guide measures designed to increase resilience. Experience suggests that many potential impacts of cascading disasters remain uninvestigated, which provides ample scope for escalation of impacts into complex forms of crisis