3 research outputs found
Visualization 2: MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon
Dysplastic colon Originally published in Biomedical Optics Express on 01 August 2015 (boe-6-8-3074
Visualization 1: MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon
Normal colon Originally published in Biomedical Optics Express on 01 August 2015 (boe-6-8-3074
Multiplexed Targeting of Barrett’s Neoplasia with a Heterobivalent Ligand: Imaging Study on Mouse Xenograft in Vivo and Human Specimens ex Vivo
Esophageal adenocarcinoma (EAC) is
a molecularly heterogeneous
disease that is rising rapidly in incidence and has poor prognosis.
We developed a heterobivalent peptide to target detection of early
Barrett’s neoplasia by combining monomer heptapeptides specific
for either EGFR or ErbB2 in a heterodimer configuration. The structure
of a triethylene glycol linker was optimized to maximize binding interactions
to the surface receptors on cells. The Cy5.5-labeled heterodimer QRH*–KSP*–E3–Cy5.5
demonstrated specific binding to each target and showed 3-fold greater
fluorescence intensity and 2-fold higher affinity compared with those
of either monomer alone. Peak uptake in xenograft tumors was observed
at 2 h postinjection with systemic clearance by ∼24 h in vivo.
Furthermore, ligand binding was evaluated on human esophageal specimens
ex vivo, and 88% sensitivity and 87% specificity were found for the
detection of either high-grade dysplasia (HGD) or EAC. This peptide
heterodimer shows promise for targeted detection of early Barrett’s
neoplasia in clinical study