18 research outputs found
The Thermal Structural Transition of α-Crystallin Inhibits the Heat Induced Self-Aggregation
-crystallin, the major constituent of human lens, is a member of the heat-shock proteins family and it is known to have a quaternary structural transition at . The presence of calcium ions and/or temperature changes induce supramolecular self-aggregation, a process of relevance in the cataractogenesis. Here we investigate the potential effect of the bovine -crystallin's structural transition on the self-aggregation process. Along all the temperatures investigated, aggregation proceeds by forming intermediate molecular assemblies that successively aggregate in clusters. The final morphology of the aggregates, above and below , is similar, but the aggregation kinetics are completely different. The size of the intermediate molecular assemblies, and their repulsive energy barrier show a marked increase while crossing . Our results highlight the key role of heat modified form of -crystallin in protecting from aggregation and preserving the transparency of the lens under hyperthermic conditions
ADP inhibits the sliding velocity of fluorescent actin filaments on cardiac and skeletal myosins.
Research on 3D geological modeling method based on section thinning-densification and close-range photogrammetry
Does phosphate release limit the ATPases of soleus myofibrils? Evidence that (A)M· ADP·Pi states predominate on the cross-bridge cycle
Risco de lesão por pressão em UTI: adaptação transcultural e confiabilidade da EVARUCI
Quantitative Structure-Retention Relationship Studies with Biopartitioning Micellar Chromatography Systems by Amended Linear Solvation Energy Relationships in Consideration of Electronic Factor
Predicting serious complications in patients with cancer and pulmonary embolism using decision tree modelling: the EPIPHANY Index
Our objective was to develop a prognostic stratification tool that enables patients with cancer and pulmonary embolism (PE), whether incidental or symptomatic, to be classified according to the risk of serious complications within 15 days. The sample comprised cases from a national registry of pulmonary thromboembolism in patients with cancer (1075 patients from 14 Spanish centres). Diagnosis was incidental in 53.5% of the events in this registry. The Exhaustive CHAID analysis was applied with 10-fold cross-validation to predict development of serious complications following PE diagnosis. About 208 patients (19.3%, 95% confidence interval (CI), 17.1-21.8%) developed a serious complication after PE diagnosis. The 15-day mortality rate was 10.1%, (95% CI, 8.4-12.1%). The decision tree detected six explanatory covariates: Hestia-like clinical decision rule (any risk criterion present vs none), Eastern Cooperative Group performance scale (ECOG-PS; We have developed and internally validated a prognostic index to predict serious complications with the potential to impact decision-making in patients with cancer and PE