2 research outputs found
A Luminescent Cocaine Detection Platform Using a Split G‑Quadruplex-Selective Iridium(III) Complex and a Three-Way DNA Junction Architecture
In this study, a series of 10 in-house
cyclometalated iridiumÂ(III)
complexes bearing different auxiliary ligands were tested for their
selectivity toward split G-quadruplex in order to construct a label-free
switch-on cocaine detection platform employing a three-way junction
architecture and a G-quadruplex motif as a signal output unit. Through
two rounds of screening, we discovered that the iridiumÂ(III) complex <b>7</b> exhibited excellent selectivity toward the intermolecular
G-quadruplex motif. A detection limit as low as 30 nM for cocaine
can be achieved by this sensing approach with a linear relationship
between luminescence intensity and cocaine concentration established
from 30 to 300 nM. Furthermore, this sensing approach could detect
cocaine in diluted oral fluid. We hope that our simple, signal-on,
label-free oligonucleotide-based sensing method for cocaine using
a three-way DNA junction architecture could act as a useful platform
in bioanalytical research
An Iridium(III) Complex Inhibits JMJD2 Activities and Acts as a Potential Epigenetic Modulator
A novel
iridiumÂ(III) complex was synthesized and evaluated for
its ability to target JMJD2 enzymatic activity. The iridiumÂ(III) complex <b>1</b> can inhibit JMJD2 activity and was selective for JMJD2 activity
over JARID, JMJD3, and HDAC activities. Moreover, <b>1</b> suppressed
the trimethylation of the p21 promoter on H3K9me3 and interrupted
the JMJD2D–H3K9me3 interactions in human cells, suggesting
that it could act as an epigenetic modulator. To our knowledge, <b>1</b> represents the first metal-based JMJD2 inhibitor reported
in the literature