Additional file 2: Figure S1. of Genome-wide analysis of microRNA targeting impacted by SNPs in cucumber genome

The heat map of miRNAs expression. (PDF 9 kb

Additional file 11: Table S8. of Genome-wide analysis of microRNA targeting impacted by SNPs in cucumber genome

The primers for amplifying precursor sequences of miRNA genes. (XLSX 18 kb

Identification of the four proteins indicated in Fig 1 from the conidia of Foc.

<p>Identification of the four proteins indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152273#pone.0152273.g001" target="_blank">Fig 1</a> from the conidia of Foc.</p

Construction, verification, and virulence analysis of complementation mutants Foc-ΔSIX1::Foc-SIX1.

<p>A: Foc-ΔSIX1 mutants were complemented by transformation with approximately 3.0 kb of <i>Foc-SIX1</i> spanning from -1838 bp (promoter relative to start codon) to 1132 bp (ORF and 3’ UTR). B: The <i>SIX1</i> gene, <i>hph</i> gene and <i>Neo</i> gene fragments were amplified by PCR to confirm the correct complementation mutants. C: Disease symptoms of cabbage seedlings inoculated with wild type isolate Foc, deletion mutants Foc-ΔSIX1, and complementation mutants Foc-ΔSIX1::Foc-SIX1 were shown at 14 dpi. Similar results were obtained in three independent biological replicates.</p

Construction and verification of deletion mutants Foc-ΔSIX1.

<p>A: <i>Foc-SIX1</i> gene deletion and replacement with an intact selectable marker gene (<i>hph</i>) by homologous recombination. B: Confirmation of the correct deletion by PCR. Numbers 1, 2, 3 and 4 represent the four primer pairs that specify the <i>SIX1</i> gene, <i>hph</i> gene, and the correct upstream and downstream homologous recombination, respectively. C: Southern hybridization analysis of wild type isolate and mutants Foc-ΔSIX1. The genomic DNAs from the wild type Foc isolate and deletion mutants Foc-ΔSIX1 were digested with <i>Sal</i> I. A fragment amplified from upstream of the target gene was used as probe.</p

Reference maps from 2-DE analysis of conidial proteins from <i>F</i>. <i>oxysporum</i> f. sp <i>conglutinans</i>.

<p>Proteins were separated by using 24 cm, pH 4–7 IPG strips for IEF and followed by 12.5% SDS-PAGE. The gels were stained by GAP method, which used CBB-G250, ammonium sulfate, and phosphoric acid. A: Gel of conidia proteins from Foc grown in PDB. B: The four proteins (spots 1, 2, 3 and 4), named Foc-SIX1 with high abundance in Foc, were indicated by arrows and numbers and listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152273#pone.0152273.t001" target="_blank">Table 1</a>; Figure B is the partial enlarged details of the rectangular box in Figure A.</p