Cyclic negative pressure promotes chondrocyte growth: Association of IGF-II with EGR-1

Knee osteoarthritis (KOA) is one of the most common degenerative joint diseases in the elderly worldwide. The primary lesion in patients with KOA is the degeneration of articular cartilage. This study aimed to observe the biological effects of cyclic negative pressure on C28/I2 chondrocytes and to elucidate the underlying molecular mechanisms. We designed a bi-directional intelligent micro-pressure control device for cyclic negative pressure intervention on C28/I2 chondrocytes. Chondrocyte vitality and proliferation were assessed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays. The extracellular matrix was analyzed using real-time fluorescence quantitative polymerase chain reaction (PCR) and western blot, while the molecular mechanism of the chondrocyte response to cyclic negative pressure was explored through mRNA sequencing. Experimental data demonstrated that cyclic negative pressure promoted chondrocyte proliferation and upregulated the expression of chondrocyte-specific protein, namely the collagen type II alpha 1 chain (COL2A1) protein, and the transcription factor SRY-box transcription factor 9 (SOX9). Additionally, RNA sequencing analysis revealed that the gene levels of insulin-like growth factor 2 (IGF-II) and early growth response 1 (EGR-1) were significantly elevated in the cyclic negative pressure group. This study demonstrates that cyclic negative pressure stimulates the proliferation of C28/I2 chondrocytes by promoting the expression of EGR-1 and IGF-II. This new discovery may provide novel insights into cartilage health and KOA prevention.

Seismic Performance of Concrete Column Connection with Square-Upper-Circular-Lower Steel Tube for Antique Buildings

The antique building combines traditional design with contemporary technology, making it an important structural style. Columns, as a crucial structural component, directly affect how well the building functions as a whole. This paper proposes a new connection form with the upper square concrete-filled steel tube-lower circular concrete-filled steel tube (USCFST-LCCFST). This study investigates the seismic performance of the proposed connection form of the columns. First, the finite element software ABAQUS-2021 is used to simulate and analyze the connection forms of the upper square concrete-filled steel tube and lower circular reinforced concrete (USCFST-LCRC) and the upper square steel reinforced concrete and lower circular reinforced concrete (USSRC-LCRC) above the antique building, respectively, which confirms the rationality of the modeling method explored in this paper. Then, geometric modeling of the USCFST-LCCFST connection is performed using ABAQUS. Simulation results demonstrate the superior seismic performance of the proposed connection form. In addition, the influence law of steel tube yield strength and the ratio of upper and lower column linear stiffness on its seismic performance are analyzed and determined through the variational parameter analysis of the USCFST-LCCFST connection form. The steel tube yield strength of USCFST-LCCFST column connection components is recommended to be 355–420 MPa and the ratio of upper and lower column linear stiffness should be no less than 0.063. In order to ensure the good seismic performance of the connection, the steel tube yield strength and the ratio of upper and lower column stiffness should be efficiently controlled in the design of antique buildings’ USCFST-LCCFST column connection components

<i>Sargassum fusiforme</i> Polysaccharide-Based Hydrogel Microspheres Enhance Crystal Violet Dye Adsorption Properties

In this study, a polysaccharide-based hydrogel microsphere (SFP/SA) was prepared using S. fusiforme polysaccharide (SFP) and sodium alginate (SA). Fourier transform infrared spectroscopy (FT-IR) demonstrated that SFP was effectively loaded onto the hydrogel microsphere. Texture profile analysis (TPA) and differential scanning calorimetry (DSC) showed that, with the increase of SFP concentration, the hardness of SFP/SA decreased, while the springiness and cohesiveness of SFP/SA increased, and the thermal stability of SFP/SA improved. The equilibrium adsorption capacity of SFP/SA increased from 8.20 mg/g (without SFP) to 67.95 mg/g (SFP accounted 80%) without swelling, and from 35.05 mg/g (without SFP) to 81.98 mg/g (SFP accounted 80%) after 24 h swelling. The adsorption of crystal violet (CV) dye by SFP/SA followed pseudo-first order and pseudo-second order kinetics (both with R2 > 0.99). The diffusion of intraparticle in CV dye was not the only influencing factor. Moreover, the adsorption of CV dye for SFP/SA (SFP accounted 60%) fit the Langmuir and Temkin isotherm models. SFP/SA exhibited good regenerative adsorption capacity. Its adsorption rate remained at > 97% at the 10th consecutive cycle while SFP accounted for 80%. The results showed that the addition of Sargassum fusiforme polysaccharide could increase the springiness, cohesiveness and thermal stability of the hydrogel microsphere, as well as improve the adsorption capacity of crystal violet dye

FOXC1 Downregulates Nanog Expression by Recruiting HDAC2 to Its Promoter in F9 Cells Treated by Retinoic Acid

FOXC1, a transcription factor involved in cell differentiation and embryogenesis, is demonstrated to be a negative regulator of Nanog in this study. FOXC1 is up-regulated in retinoic acid-induced differentiation of F9 Embryonal Carcinoma (EC) cells; furthermore, FOXC1 specifically inhibits the core pluripotency factor Nanog by binding to the proximal promoter. Overexpression of FOXC1 in F9 or knockdown in 3T3 results in the down-regulation or up-regulation of Nanog mRNA and proteins, respectively. In order to explain the mechanism by which FOXC1 inhibits Nanog expression, we identified the co-repressor HDAC2 from the FOXC1 interactome. FOXC1 recruits HDAC2 to Nanog promoter to decrease H3K27ac enrichment, resulting in transcription inhibition of Nanog. To the best of our knowledge, this is the first report that FOXC1 is involved in the epigenetic regulation of gene expression

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H₂O₂ Treatment

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H2O2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H2O2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides

Influence of PEG Stoichiometry on Structure-Tuned Formation of Self-Assembled Submicron Nickel Particles

Self-assembled submicron nickel particles were successfully synthesized via the one-step surfactant-assisted solvothermal method. The impact of surfactant and reducing agent stoichiometry is investigated in this manuscript. Different morphologies and structures of Ni particles, including flower-like nanoflakes, hydrangea-like structures, chain structures, sphere-like structures, and hollow structures were prepared through different processing conditions with two parameters such as temperature and time. Based on scanning electron microscopy (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA) and vibrating sample magnetometry (VSM), the submicron nickel particles show good saturation magnetization and excellent thermal stabilities with a possible growth mechanism for the variety of the structure-tuned formation. Importantly, the microwave absorption properties of the submicron nickel particles were studied. The lowest reflection loss of Ni-P9/T200/H15 with a thin layer thickness of 1.7 mm can reach −42.6 dB at 17.3 GHz

In situ encapsulated molybdovanaphosphodic acid on modified nanosized TS-1 zeolite catalyst for deep oxidative desulfurization

In order to solve air pollution caused by SOx, oxidative deep desulfurization of diesel, has become a hotspot all over the world. In this paper, molybdovanaphosphodic acid (HPV2Mo10), a typical polyoxometalate (POM), was in-situ impregnated on TS-1-TAPOH@SiO2 which originated from TS-1 modified by tetrapropylammonium hydroxide (TPAOH) and tetraethyl orthosilicate (TEOS). The catalysts were characterized by X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Ultraviolet-Visible Diffuse Reflectance (UV-vis), Raman Spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), N-2-physisorption, X-ray Photoelectron Spectroscopy (XPS), Inductively Coupled Plasma Spectroscopy (ICP), P-31 and Si-29 Magic Angle Spinning-Nuclear Magnetic Resonance (MAS-NMR). The characterization results of XRD, FT-IR, UV-vis, Raman and MAS-NMR showed that HPV2Mo10 was successfully loaded on TS-1-TPAOH@SiO2, while the structure of support and Keggin-type of HPV2Mo10 remained unchanged. The catalysts were applied to the oxidative desulfurization (ODS) and adsorption desulfurization of thiophene (TH), benzothiophene (BT), and dibenzothiophene (DBT). The results show that HPV2Mo10/TS-1-TPAOH@SiO2 catalyst prepared by in-situ method has the best oxidation activity when the structure of both the POM and modified zeolite are retained. Under the selected reaction condition: T = 70 degrees C, n(H2O2)/n(Sulfur) = 13, m (oil): m (catalyst )= 35: 1. The conversion rates of TH, BT and DBT over HPV2Mo10/TS-1-TPAOH@SiO2-in situ after 3 h were 99.3%, 45.9% and 84.1%, respectively. The function of two part in the investigated HPV2Mo10/TS-1-TPAOH@SiO2 catalyst, known as the modified nanosized TS-1 zeolite and HPV2Mo10, for the oxidative desulfurization activity are demonstrated