29 research outputs found
Assessing the impact of curcumin on dualâspecies biofilms formed by Streptococcus mutans and Candida albicans
Streptococcus mutans and Candida albicans are often isolated from plaques associated with early childhood caries. However, there are limited studies examining how these microorganisms interact with one another and how best to manage them. Recent studies have shown that curcumin (CUR), a natural compound, has the potential to independently control both of these microorganisms. The purpose of this study was to investigate how S. mutans and C. albicans respond in monoâ and dualâspecies biofilms challenged with CUR. Quantitative biofilm biomass and viability were first evaluated and supported by liveâdead PCR to assess biofilm composition. Confocal laser scanning microscopy (CLSM) was used to evaluate the exopolysaccharide (EPS) content and thickness of the biofilms, and the structure of the biofilms and morphology of the cells were observed by scanning electron microscopy (SEM). Quantitative realâtime PCR (qRTâPCR) was applied to assess relative gene expression. The 50% minimum biofilm eradication concentration (MBEC50) of CUR against S. mutans and C. albicans was 0.5 mM. The biomass and viability decreased after treatment with CUR both in dualâspecies biofilms and in monoâspecies biofilm. CUR inhibited S. mutans and C. albicans in both monoâ and dualâspecies biofilms. Streptococcus mutans was more sensitive to CUR in dualâspecies biofilm than in monoâspecies biofilms, whereas C. albicans was less sensitive in dualâspecies biofilms. EPS production was decreased by CUR in both monoâ and dualâspecies biofilms, which coincided with the downregulation of glucosyltransferase and quorum sensingârelated gene expression of S. mutans. In C. albicans, the agglutininâlike sequence family of C. albicans was also downregulated in dualâspecies biofilms. Collectively, these data show the potential benefit of using a natural antimicrobial, CUR, to control cariesârelated dualâspecies plaque biofilms
Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation in S. mutans
Streptococcus mutans (S. mutans) has been proved to be the main aetiological factor in dental caries. Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest. The objective of this study is to evaluate the inhibitory effects of curcumin on metabolism and biofilm formation in S. mutans using a vitro biofilm model in an artificial oral environment. S. mutans biofilms were treated with varying concentrations of curcumin. The biofilm metabolism and biofilm biomass were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the crystal violet assay. Confocal laser scanning microscopy was used to analyse the composition and extracellular polysaccharide content of S. mutans biofilm after curcumin treatment. The biofilm structure was evaluated using a scanning electron microscope. The gene expression of virulence-related factors was assessed by real-time PCR. The antibiofilm effect of curcumin was compared with that of chlorhexidine. The sessile minimum inhibitory concentration (SMIC50%) of curcumin against S. mutans biofilm was 500âÎŒM. Curcumin reduced the biofilm metabolism from 5âmin to 24âh. Curcumin inhibited the quantity of live bacteria and total bacteria in both the short term (5âmin) and the long term. Moreover, curcumin decreased the production of extracellular polysaccharide in the short term. The expression of genes related to extracellular polysaccharide synthesis, carbohydrate metabolism, adherence, and the two-component transduction system decreased after curcumin treatment. The chlorhexidine-treated group showed similar results. We speculate that curcumin has the capacity to be developed as an alternative agent with the potential to reduce the pathogenic traits of S. mutans biofilm
Forecasting of Chinese Primary Energy Consumption in 2021 with GRU Artificial Neural Network
The forecasting of energy consumption in China is a key requirement for achieving national energy security and energy planning. In this study, multi-variable linear regression (MLR) and support vector regression (SVR) were utilized with a gated recurrent unit (GRU) artificial neural network of Chinese energy to establish a forecasting model. The derived model was validated through four economic variables; the gross domestic product (GDP), population, imports, and exports. The performance of various forecasting models was assessed via MAPE and RMSE, and three scenarios were configured based on different sources of variable data. In predicting Chinese energy consumption from 2015 to 2021, results from the established GRU model of the highest predictive accuracy showed that Chinese energy consumption would be likely to fluctuate from 2954.04 Mtoe to 5618.67 Mtoe in 2021
Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice
Aging is a process associated with blood–brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of Fe3O4 nanozymes on neurogenesis have rarely been studied. This study examined the effects of Fe3O4 nanozymes on neuronal differentiation in the dentate gyrus (DG) and BBB integrity of D-galactose-induced aged mice. Long-term treatment with Fe3O4 nanozymes (10 μg/mL diluted in ddH2O daily) markedly increased the doublecortin (DCX) immunoreactivity and decreased BBB injury induced by D-galactose treatment. In addition, the decreases in the levels of antioxidant proteins including superoxide dismutase (SOD) and catalase as well as autophagy-related proteins such as Becin-1, LC3II/I, and Atg7 induced by D-galactose treatment were significantly ameliorated by Fe3O4 nanozymes in the DG of the mouse hippocampus. Furthermore, Fe3O4 nanozyme treatment showed an inhibitory effect against apoptosis in the hippocampus. In conclusion, Fe3O4 nanozymes can relieve neuroblast damage and promote neuroblast differentiation in the hippocampal DG by regulating oxidative stress, apoptosis, and autophagy
Identification of novel key markers that are induced during traumatic brain injury in mice
Background Traumatic brain injury (TBI) has emerged as an increasing public health problem but has not been well studied, particularly the mechanisms of brain cellular behaviors during TBI. Methods In this study, we established an ischemia/reperfusion (I/R) brain injury mice model using transient middle cerebral artery occlusion (tMCAO) strategy. After then, RNA-sequencing of frontal lobes was performed to screen key inducers during TBI. To further verify the selected genes, we collected peripheral blood mononuclear cells (PBMCs) from TBI patients within 24 h who attended intensive care unit (ICU) in the Affiliated Hospital of Yangzhou University and analyzed the genes expression using RT-qPCR. Finally, the receiver operator characteristic (ROC) curves and co-expression with cellular senescence markers were applied to evaluate the predictive value of the genes. Results A total of six genes were screened out from the RNA-sequencing based on their novelty in TBI and implications in apoptosis and cellular senescence signaling. RT-qPCR analysis of PBMCs from patients showed the six genes were all up-regulated during TBI after comparing with healthy volunteers who attended the hospital for physical examination. The area under ROC (AUC) curves were all >0.7, and the co-expression scores of the six genes with senescence markers were all significantly positive. We thus identified TGM1, TGM2, ATF3, RCN3, ORAI1 and ITPR3 as novel key markers that are induced during TBI, and these markers may also serve as potential predictors for the progression of TBI
Influence of reaction conditions on one-step hydrotreatment of lipids in the production of iso-alkanes over Pt/SAPO-11
A one-step hydrotreatment of soybean oil to produce iso-alkanes has been carried out over a Pt/SAPO-11 catalyst in a fixed-bed reactor. The influence of reaction conditions such as temperatare, pressure, hydrogen/oil ratio, and space velocity on the catalytic performance has been investigated. From the levels of intermediate products, gas products (CO, CO2) and the target product (alkane), it was found that the hydrogenolysis of esters, fatty acids and fatty aldehydes and the formation of fatty alcohols were prohibited, which led to high selectivity for hydrodecarboxylation and hydrodecarbonylation at high temperature, low pressure or low hydrogen/oil ratio. The selectivity of the isomerization increased when temperature increased or space velocity decreased, while pressure and hydrogen/oil ratio were found to have no influence. A plausible reaction pathway has been proposed based on these results. (c) 2013, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved
Quantification of the Environmental Impacts of Highway Construction Using Remote Sensing Approach
Highways provide key social and economic functions but generate a wide range of environmental consequences that are poorly quantified and understood. Here, we developed a beforeâduringâafter control-impact remote sensing (BDACI-RS) approach to quantify the spatial and temporal changes of environmental impacts during and after the construction of the Wujing Highway in China using three buffer zones (0â100 m, 100â500 m, and 500â1000 m). Results showed that land cover composition experienced large changes in the 0â100 m and 100â500 m buffers while that in the 500â1000 m buffer was relatively stable. Vegetation and moisture conditions, indicated by the normalized difference vegetation index (NDVI) and the normalized difference moisture index (NDMI), respectively, demonstrated obvious degradationârecovery trends in the 0â100 m and 100â500 m buffers, while land surface temperature (LST) experienced a progressive increase. The maximal relative changes as annual means of NDVI, NDMI, and LST were about â40%, â60%, and 12%, respectively, in the 0â100m buffer. Although the mean values of NDVI, NDMI, and LST in the 500â1000 m buffer remained relatively stable during the study period, their spatial variabilities increased significantly after highway construction. An integrated environment quality index (EQI) showed that the environmental impact of the highway manifested the most in its close proximity and faded away with distance. Our results showed that the effect distance of the highway was at least 1000 m, demonstrated from the spatial changes of the indicators (both mean and spatial variability). The approach proposed in this study can be readily applied to other regions to quantify the spatial and temporal changes of disturbances of highway systems and subsequent recovery