Additional file 7: Table S7. of A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

The BLASTN searching results of 11542 BESs with Mx23Hm database. (XLS 479 kb

Additional file 5: Table S5. of A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

The BLASTX searching results of 4428 BESs with protein databases of S. lycopersicum. (XLS 416 kb

Additional file 12: Figure S2. of A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition

PCR amplification of Xu 781 (I) and Xushu 18 (II) and their 168 F1 individuals with BES_SSR_267. M: BL 2000 DNA Marker; Lanes 1 to 168: 168 individuals. (TIF 1647 kb

Primers used for plasmid construction and qPCR analysis.

<p>Primers used for plasmid construction and qPCR analysis.</p

Decolorization of different dyes by LacTT.

<p>(a) Decolorization of synthetic dyes by LacTT at 70°C for 24 h. (b) Decolorization of RBBR by LacTT with different concentrations of NaCl at 70°C for 24 h. The reactions were performed in 50 mM Na₂HPO₄–NaH₂PO₄ buffer (pH 7.5, but pH 8.0 for CR decolorization), 50 mg/L dye, 10 μM CuSO_4, and purified LacTT (40 U/L). The error bars represent the standard deviation.</p

Effects of halides on LacTT activity.

<p>The enzyme was incubated in 50 mM Na₂HPO₄-NaH₂PO₄ (pH 7.5, supplemented with 10 μM CuSO₄), using guaiacol as the substrate. The error bars represent the standard deviation.</p

Effect of pH on the activity (a) and stability (b) of purified LacTT at 90°C.

<p>(a) ABTS, pH 3.0–6.0; SGZ, pH 5.5–8.5; guaiacol, pH 6.5–10.0; 2, 6-DMP, pH 5.0–10.0. (b) Investigation of the pH stability of LacTT by measuring the enzyme activity at 90°C with guaiacol as the substrate. The data represent the average values from triplicate measurements. The error bars represent the standard deviation.</p

Time course of laccase production during shake flask cultivation and 10-L fed-batch fermentation.

<p>(a) Progress curves constructed in the induction phase of shake flask cultivation for the determination of the cell density of <i>P</i>. <i>pastoris/LacTT</i> (closed square) and <i>P</i>. <i>pastoris/</i>(<i>LacTT</i>)₄ (open square), volumetric activity of <i>P</i>. <i>pastoris/LacTT</i> (closed circle) and <i>P</i>. <i>pastoris/</i>(<i>LacTT</i>)₄ (open circle), specific activity of <i>P</i>. <i>pastoris/LacTT</i> (closed triangle) and <i>P</i>. <i>pastoris/</i>(<i>LacTT</i>)₄ (open triangle). (b) Cell density (closed square), volumetric activity (closed circle), and specific activity (closed triangle) of <i>P</i>. <i>pastoris/</i>(<i>LacTT</i>)₄ in fed-batch fermentation. (c) SDS-PAGE image of LacTT supernatant expressed in <i>P</i>. <i>pastoris/</i>(<i>LacTT</i>)₄ in fed-batch fermentation. Lane M: protein marker; Lane 1: 0.5 mg/mL BSA; Lane 2: 0.3 mg/mL BSA; Lane 3: 0.1 mg/mL BSA; Lane 4: Enzyme supernatant was 1:10 diluted. The error bars represent the standard deviation.</p

Effect of temperature on the activity and stability of the purified laccase.

<p>(a) Optimal temperature (open circles) was determined at pH 7.5 by using guaiacol as the substrate. Residual activity (closed circles) was determined after incubation at 40°C–90°C for 1 h. (b) Residual activity was determined after incubation at 70°C, 80°C, and 90°C, respectively, for 0–4 h. The graphs display the average values from triplicate measurements. The error bars represent the standard deviation.</p

NCTD enhances LPS induced cytokines expression at mRNA level.

<p>A, The cytotoxicity of NCTD is much lower than CTD. RAW264.7 cells were treated with NCTD or CTD for 24 h and cell viability was tested with MTS assay as described under Materials and Methods. B, Cytokines production was enhanced obviously by NCTD in RAW264.7 cells. The RAW264.7 cells were pretreated with PBS (containing 0.1% DMSO) or 1–10 μM NCTD for 24 h before stimulation with 100 ng/ml LPS for 1 h. Total cellular RNA were collected and subjected to Real time -PCR analysis. C, Only LPS induced cytokine production was enhance by NCTD obviously. The RAW264.7 cells were pretreated with 0.1% DMSO, 5 μM NCTD for 24 h before stimulation with LPS (100 ng/ml), PMA (100nM) or LTA (10 µg/ml) for 1 h. Total cellular RNA were collected and subjected to Real time PCR analysis. Columns, mean from three independent experiments with three duplicates; bars, SE (*, P<0.05; **, P<0.01 versus control).</p