EMSA showed the activity of NF-κB p65 in ACC-M, ACC-M/IκBαM, ACC-2 and ACC-2/IκBαM cell lines

<p><b>Copyright information:</b></p><p>Taken from "In vitro angiogenesis and expression of nuclear factor κB and VEGF in high and low metastasis cell lines of salivary gland Adenoid Cystic Carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/7/95</p><p>BMC Cancer 2007;7():95-95.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903362.</p><p></p> indicate the migration of the induced NF-κB DNA-binding complexes. Migration of the free probe is not shown. The Oct-1 motif was used as a control for quality and quantity of cell extract

RT-PCR showed the mRNA expression of VEGF in ACC-M, ACC-2, ACC-M/IκBαM and ACC-2/IκBαM cell lines

<p><b>Copyright information:</b></p><p>Taken from "In vitro angiogenesis and expression of nuclear factor κB and VEGF in high and low metastasis cell lines of salivary gland Adenoid Cystic Carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/7/95</p><p>BMC Cancer 2007;7():95-95.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903362.</p><p></p

Immuofluorescence double staining and semi-quantitative confocal laser scanning analysis showed NF-κB p65 and VEGF expressed in ACC-M and ACC-2 cell lines

<p><b>Copyright information:</b></p><p>Taken from "In vitro angiogenesis and expression of nuclear factor κB and VEGF in high and low metastasis cell lines of salivary gland Adenoid Cystic Carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/7/95</p><p>BMC Cancer 2007;7():95-95.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903362.</p><p></p> As figure 3A showed, the rate of NF-κB p65 nuclear localization (a) (white arrow) and VEGF staining intensity (b) in ACC-M was higher than that in ACC-2 (e) and (f). As figure 3B shows, bars represent the mean value of immunofluorescence intensity of VEGF and nuclear staining rate of NF-κB p65 in two cell lines, < 0.01(*)

Does the Biosocial Model Explain the Emergence of Status Differences in Conversations among Unacquainted Men?

<div><p>Fifteen triads of unacquainted men conversed for ten minutes while stress was measured in real time by pulse rate and thumb blood volume (TBV). Salivary measures of testosterone (T), cortisol (C), and the stress-related enzyme alpha-amylase (AA) were measured at the beginning and end of the session. Fully or partially transitive status hierarchies formed in 14 triads. (Highest ranked man was scored 1, lowest 3, with ties allowed.) Ten of the triads participated in Study 1, where nothing was at stake in the casual conversation. Five additional triads were run in Study 2, intended to introduce competition by offering a $20 reward to the man afterward chosen as having led the conversation. Most results from the two studies are similar, suggesting that the$20 reward had little effect. Combining studies, pulse and TBV show that conversation is more stressful than watching a video beforehand. Within the conversation, speaking turns are more stressful than listening turns, especially among the lowest ranked men, less so among those higher in rank. This supports a stress-based mechanism for status allocation among humans. Apparently, human speech is a form of status signaling, homologous with nonlinguistic status signals used by other primates, as posited by the “biosocial model.” The biosocial model also posits that a physiological substrate (T, C, and AA) is related to dominance or status. Predicted effects are not replicated here, except for an inverse relationship between the stress enzyme AA and status. The mostly null results, obtained from conversations where there was little or nothing at stake, suggest that T and C (and their interaction) are not relevant to emergent status in the absence of serious competition.</p></div

Relationships of status rank to prior-to-post changes in lnT, C, and lnAA.

<p>Relationships of status rank to prior-to-post changes in lnT, C, and lnAA.</p

Talking/listening ratio of TBV is higher, and of pulse rate is lower, among high-ranked men (combining studies).

<p>Talking/listening ratio of TBV is higher, and of pulse rate is lower, among high-ranked men (combining studies).</p

The biosocial model.

<p>The biosocial model.</p

Mean LnT, C, and LnAA from prior and post saliva samples^*.

<p>*Differences from prior to post are not significant by paired-comparison t-tests. Post and prior lnAA are significantly lower in Study 2 than in Study 1 (t-tests); values of lnT and C are not.</p><p>Mean LnT, C, and LnAA from prior and post saliva samples^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142941#t001fn001" target="_blank">*</a>}.</p

Hormones and AA as functions of competition (Study 1 vs Study 2), time, and status rank.

<p>Hormones and AA as functions of competition (Study 1 vs Study 2), time, and status rank.</p

Multilevel models of status rank as a function of lnT, C, and lnAA in Studies 1 and 2.

<p>Multilevel models of status rank as a function of lnT, C, and lnAA in Studies 1 and 2.</p