British carboniferous bryozoan biogeography

The geographical and temporal distributions of the British Carboniferous Bryozoa have been determined, incorporating data from fieldwork (including localities in Scotland, North England, North Wales, South Wales, and Avon), museum collections, and literature searches. A total of 126 species has been recognised, though further work may reveal some synonymies within this list. The forty species collected during the fieldwork have been identified by reference to previous taxonomic work; most species can be assigned to established taxonomic descriptions, but two new species, Rhombopora bancrofti and Polypora hexagonaria, are proposed, and the descriptions of Rhombopora incrassata and Rhombopora similis are emended. The applicability of Student's t-test to the morphometric analysis of Carboniferous bryozoan species has been studied, and a computer program written to perforin this task, incorporating a database of species measurements. A new statistical method, the division t-test, is presented in this thesis; this method is useful in comparing the relative ratios of bryozoan colony parameters. Both the t-test and the division t-test were found to be of use in identifying bryozoan species. Analysis of the bryozoan faunas in nine regional areas of Britain has been made using the Simpson and Jaccard coefficients of similarity. The resulting coefficients are consistent with the limited distribution capability of many bryozoan taxa, and also match with the known palaeogeography and palaeocurrents of the Carboniferous of northwest Europe. Plots of species diversity against geographical distribution show a similar pattern to that produced by Tertiary non-planktotrophic larvae-bearing neogastropods. Further, additional plots of species diversity against species longevity produced a pattern consistent with normal background extinction events. A study of bryozoan morphology between different areas and different stages within the Carboniferous indicated that species showed no measurable temporal evolutionary or lateral geographical changes through the Lower Carboniferous. Rather, local environmental stresses are the major architects of bryozoan colony morphology

Are spherulitic lacustrine carbonates an expression of large-scale mineral carbonation? : A case study from the East Kirkton Limestone, Scotland

BP Exploration Co. is thanked for funding, and particularly the Carbonate Team for supporting this research and for fruitful discussions. West Lothian Council and Scottish Natural Heritage are thanked for allowing access and permission for sampling the site. The Core Store Team at BGS Keyworth is particularly acknowledged for their assistance. Mark Anderson, Tony Sinclair (University of Hull), and Bouk Lacet (VU University Amsterdam) are thanked for technical support. Anne Kelly (SUERC) for carrying out the Strontium Isotope analyses. Mark Tyrer is thanked for his advice on PHREEQC modelling.Peer reviewedPostprin

The Telehealth Skills, Training, and Implementation Project: An evaluation protocol

External stabilization is reported to improve reliability of hand held dynamometry, yet this has not been tested in burns. We aimed to assess the reliability of dynamometry using an external system of stabilization in people with moderate burn injury and explore construct validity of strength assessment using dynamometry. Participants were assessed on muscle and grip strength three times on each side. Assessment occurred three times per week for up to four weeks. Within session reliability was assessed using intraclass correlations calculated for within session data grouped prior to surgery, immediately after surgery and in the sub-acute phase of injury. Minimum detectable differences were also calculated. In the same timeframe categories, construct validity was explored using regression analysis incorporating burn severity and demographic characteristics. Thirty-eight participants with total burn surface area 5 – 40% were recruited. Reliability was determined to be clinically applicable for the assessment method (intraclass correlation coefficient \u3e0.75) at all phases after injury. Muscle strength was associated with sex and burn location during injury and wound healing. Burn size in the immediate period after surgery and age in the sub-acute phase of injury were also associated with muscle strength assessment results. Hand held dynamometry is a reliable assessment tool for evaluating within session muscle strength in the acute and sub-acute phase of injury in burns up to 40% total burn surface area. External stabilization may assist to eliminate reliability issues related to patient and assessor strength

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children

Indices of physiological strain for firefighters of the Australian Defence Forces

High levels of exertion and physiological strain are the leading cause of fireground injuries. The Physiological Strain Index (PSI) provides a rating of strain based on body core temperature and heart rate; however, it may underestimate the strain of workers in protective clothing as skin temperature may be elevated. This study aimed to examine the relationship between the PSI and an Adaptive Physiological Strain Index (aPSI) that incorporates skin temperature, among firefighters wearing protective clothing. Nine male firefighters of the Australian Defence Force volunteered to participate. Participants conducted scenario-based activities while wearing turnout gear and breathing apparatus. Working in teams of four, participants would respond to a situation around and within a small building with several rooms that could be filled with smoke, however, no live fire was present. Heart rate, gastrointestinal temperature, and skin temperature were monitored throughout work and rehabilitation. Physiological strain was estimated via the PSI and aPSI. Absolute peak PSI and aPSI ratings were significantly different during work (PSI: 7.3?±?1.6; aPSI 8.2?±?2.0; p?0.5 above PSI, progressively from a moderate level of strain (>6). The aPSI may provide a more accurate indication of a level of ?maximal strain? for encapsulated workers than the original PSI, coincident with the occupational limits for body core temperature of 38.0?°C for general occupational groups, or 38.5?°C for selected and acclimatised personnel

Towards a morphology diagram for terrestrial carbonates: evaluating the impact of carbonate supersaturation and alginic acid in calcite precipitate morphology

Ancient and recent terrestrial carbonate-precipitating systems are characterised by a heterogeneous array of deposits volumetrically dominated by calcite. In these environments, calcite precipitates display an extraordinary morphological diversity, from single crystal rhombohedral prisms, to blocky crystalline encrustations, or spherulitic to dendritic aggregates. Despite many decades of thorough descriptive and interpretative work on these fabrics, relating calcite micro-morphology with sedimentary hydrogeochemical conditions remains a challenge. Environmental interpretations have been hampered by the fact that calcite morphogenesis results from the complex interaction between different physico-chemical parameters which often act simultaneously (e.g., carbonate mineral supersaturation, Mg/Ca ratio of the parental fluid, organic and inorganic additives). To try to experimentally address the sedimentological causes of calcite morphogenesis, an experimental approach yielding a first attempt at a calcite growth-form phase diagram is presented here. The initial aim was to account for the carbonate products experimentally nucleated in alkaline, saline lake settings. These are the result of at least two competing calcite precipitation ‘driving forces’ that affect morphogenesis: the calcite supersaturation level of the parental fluid, and the concentration of microbial-derived organic molecules (alginic acid). A key finding of this study is that common naturally-occurring calcite products such as calcite floating rafts, rhombohedral prismatic forms, di-pyramid calcite crystals, spherulitic calcite grains, or vertically stacked spheroidal calcite aggregates, can be related to specific hydrogeochemical contexts, and their physical transitions pinpointed in a phase diagram. By exploring binary or ternary responses to forcing in morphological phase-space, links between calcite growth forms and (palaeo)environmental conditions can be determined. This provides a truly process-oriented means of navigating questions around carbonate precipitate morphogenesis for the future

Assessment of thrombin generation measured before and after cardiopulmonary bypass surgery and its association with postoperative bleeding

Background: Bleeding after cardiopulmonary bypass (CPB) is a major cause of morbidity and mortality and consumes large amounts of blood. Identifying patients at increased risk of bleeding secondary to hemostatic impairment may improve clinical outcomes by allowing early intervention. Methods: This present study recruited 77 patients undergoing CPB and measured coagulation screens, coagulation factors, TEG®, Rotem® and thrombin generation (TG) before surgery and 30 min after heparin reversal. The tests were analyzed to investigate whether they identified patients at increased risk of excess bleeding (defined as > 1000 mL) in the first 24 h postoperatively. Results: Patients who bled > 1000 mL had a lower: platelet count (P < 0.02), factors (F)IX, X and XI (P < 0.005), endogenous thrombin potential (ETP) and an initial rate of TG (P < 0.02) and higher activated partial thromboplastin time (aPTT) (P < 0.001) than patients who bled < 1000 mL. Receiver operating characteristic (ROC) analysis was significant for post-operative TG and aPTT (P < 0.001). Furthermore, reduced pre-operative TG was associated with increased postoperative bleeding (P < 0.02). Pre- and postoperative TG were correlated (q = 0.7, P < 0.001). TEG®, Rotem® and prothrombin time (PT) at either time point were not associated with increased bleeding. Conclusion: These data suggest that pre-operative defects in the propagation phase of hemostasis are exacerbated during CPB, contributing to bleeding post-CPB. TG taken both pre- and postoperatively could potentially be used to identify patients at an increased risk of bleeding post-CPB