Nasal carriage of S.aureus in children with allergic rhinitis

INTRODUCTION: INTRODUCTION: Allergic rhinitis is an Ig E mediated mucosal inflammation of the nasal mucosa. S.aureus may be identified as a coloniser of the nasal flora of heathy individuals. In this study, we aimed to evaluate the effect of topical mometazon furoat (MF) usage on nasal S.aureus carriage among patients with allergic rhinitis. METHODS: METHODS: Our study included 44 newly diagnosed allegic rhinitis patients never used drugs previously, 45 patients whom have been using MF minimum of six months and 27 healthy children as control group. All volunteers' gave nasal samples via Stuart transport swab and samples cultured and incubated to agar for 24 to 48 hours. Identification of the colonies performed via conventional methods and VITEK®2 Compact. RESULTS: RESULTS: The percentages of positive S.aureus nazal culture detected 40.9, 48.9 and 11.1 for newly diagnosed allegic rhinitis cases, patients using MF minimum of six months and healthy control group, respectively. DISCUSSION AND CONCLUSION: DISCUSSION AND CONCLUSION: The allergic rhinitis seems to increase nasal S.aureus colonisation significantly, but nasal MF don't increase the possibility of this colonisation

Investigation of Extended Spectrum Beta-Lactamase (ESBL) Genes in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Strains

Introduction: Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is an important health problem all over the world. In this study, it was aimed to determine the ESBL genes in Escherichia coli and Klebsiella pneumoniae strains isolated for approximately four-year period. Materials and Methods: A total 100 ESBL-producing E. coli and 100 ESBL-producing K. pneumoniae strains which were isolated between January 2008 and October 2012 were included into this study. The strains were identified using classical bacteriologic methods and BD Phoenix (Becton Dickinson, US) automatized bacterial identification device. CTX-M, TEM, SHV, VEB, GES, PER and OXA beta-lactamase genes were analyzed with the PCR method. Results: The beta-lactamase genes detected in ESBL-positive K. pneumoniae strains were as follows: 99% for CTX-M, 91% for SHV, 71% for TEM, 10% for OXA-10 group, and 5% for OXA-2 group. In E. coli strains, the prevalence of CTX-M was 92%; TEM was 70%, SHV was 21%, and OXA-2 group was 3%. CTX-M alone was found to be positive in 25 of the 98 (25.5%) in E. coli strains; TEM alone was found to be positive in 2 of 98 (2%) and SHV alone was found in 2 of 98 (2%). CTX-M alone was found positive in 3 of 100 (3%) K. pneumoniae strains. No other resistance genes alone were found in the strains. No GES, VEB and PER-producing strains were determined in this study. Conclusion: In the study, high prevalence of CTX-M beta-lactamase was found in ESBL-producing strains. It was thought that the high potential of mobility with CTX-M genes was the most possible reason for this result. Determination of ESBL genes will be useful to understand resistance epidemiology, develop effective therapeutic strategies, and plan the appropriate preventive measurements