Pseudorabies Virus Infection Alters Neuronal Activity and Connectivity In Vitro

Alpha-herpesviruses, including human herpes simplex virus 1 & 2, varicella zoster virus and the swine pseudorabies virus (PRV), infect the peripheral nervous system of their hosts. Symptoms of infection often include itching, numbness, or pain indicative of altered neurological function. To determine if there is an in vitro electrophysiological correlate to these characteristic in vivo symptoms, we infected cultured rat sympathetic neurons with well-characterized strains of PRV known to produce virulent or attenuated symptoms in animals. Whole-cell patch clamp recordings were made at various times after infection. By 8 hours of infection with virulent PRV, action potential (AP) firing rates increased substantially and were accompanied by hyperpolarized resting membrane potentials and spikelet-like events. Coincident with the increase in AP firing rate, adjacent neurons exhibited coupled firing events, first with AP-spikelets and later with near identical resting membrane potentials and AP firing. Small fusion pores between adjacent cell bodies formed early after infection as demonstrated by transfer of the low molecular weight dye, Lucifer Yellow. Later, larger pores formed as demonstrated by transfer of high molecular weight Texas red-dextran conjugates between infected cells. Further evidence for viral-induced fusion pores was obtained by infecting neurons with a viral mutant defective for glycoprotein B, a component of the viral membrane fusion complex. These infected neurons were essentially identical to mock infected neurons: no increased AP firing, no spikelet-like events, and no electrical or dye transfer. Infection with PRV Bartha, an attenuated circuit-tracing strain delayed, but did not eliminate the increased neuronal activity and coupling events. We suggest that formation of fusion pores between infected neurons results in electrical coupling and elevated firing rates, and that these processes may contribute to the altered neural function seen in PRV-infected animals

Badania kariologiczne niektórych mieszańców międzygatunkowych z rodzaju Solanum [Cytological studies of some interspecies hybrids of the genus Solanum]

Karyological analysis of interspecies hybrids of tetraploid (2n=48) varieties of Solanum tuberosum (Flisak, Fortuna, Sagitta and Spekula) and di-haploid form of S. tuberosum (2n=24) with semicultivated species S. phureja (2n=24), wild diploid species S. chacoense (2n=24), S. simplicifolium (2n=24), S. vernei (2n=24) and tetraploid species S. stoloniferum (2n=48) was carried out

Laser Welding Of Finned Tubes Made Of Austenitic Steels

This paper describes the technology of welding of finned tubes made of the X5CrNi1810 (1.4301) austenitic steel, developed at Energoinstal SA, allowing one to get high quality joints that meet the requirements of the classification societies (PN-EN 15614), and at the same time to significantly reduce the manufacturing costs. The authors described an automatic technological line equipped with a Trumph disc laser and a tube production technological process. To assess the quality of the joints, one performed metallographic examinations, hardness measurements and a technological attempt to rupture the fin. Analysis of the results proved that the laser-welded finned tubes were performed correctly and that the welded joints had shown no imperfections.W pracy przedstawiono technologię spawania rur ożebrowanych ze stali austenicznej X5CrNi1810 (1.4301), opacowaną w firmie Energoinstal SA zapewniającą uzyskanie wysokiej jakości połączeń spełniających wymagania towarzystw kwalifikacyjnych (PN-EN 15614), przy istotnym obniżeniu kosztów wytwarzania. Opisano automatyczną linię technologiczną wyposażoną w laser dyskowy firmy Trumph oraz proces technologiczny produkcji rur. Do oceny jakości złączy spawanych wykonano badania metalograficzne, pomiary twardości oraz technologiczną próbę odrywania żebra. Na podstawie analizy wyników stwierdzono, że rury ożebrowane spawane laserowo są wykonanae prawidowo, a w złączach spawanych nie ujawniono niezgodności

Laser Welding Of Finned Tubes Made Of Austenitic Steels

This paper describes the technology of welding of finned tubes made of the X5CrNi1810 (1.4301) austenitic steel, developed at Energoinstal SA, allowing one to get high quality joints that meet the requirements of the classification societies (PN-EN 15614), and at the same time to significantly reduce the manufacturing costs. The authors described an automatic technological line equipped with a Trumph disc laser and a tube production technological process. To assess the quality of the joints, one performed metallographic examinations, hardness measurements and a technological attempt to rupture the fin. Analysis of the results proved that the laser-welded finned tubes were performed correctly and that the welded joints had shown no imperfections

Spawanie laserowe rur ożebrowanych ze stali austenicznych

This paper describes the technology of welding of finned tubes made of the X5CrNi1810 (1.4301) austenitic steel, developed at Energoinstal SA, allowing one to get high quality joints that meet the requirements of the classification societies (PN-EN 15614), and at the same time to significantly reduce the manufacturing costs. The authors described an automatic technological line equipped with a Trumph disc laser and a tube production technological process. To assess the quality of the joints, one performed metallographic examinations, hardness measurements and a technological attempt to rupture the fin. Analysis of the results proved that the laser-welded finned tubes were performed correctly and that the welded joints had shown no imperfections.W pracy przedstawiono technologię spawania rur ożebrowanych ze stali austenicznej X5CrNi1810 (1.4301), opacowaną w firmie Energoinstal SA zapewniającą uzyskanie wysokiej jakości połączeń spełniających wymagania towarzystw kwalifikacyjnych (PN-EN 15614), przy istotnym obniżeniu kosztów wytwarzania. Opisano automatyczną linię technologiczną wyposażoną w laser dyskowy firmy Trumph oraz proces technologiczny produkcji rur. Do oceny jakości złączy spawanych wykonano badania metalograficzne, pomiary twardości oraz technologiczną próbę odrywania żebra. Na podstawie analizy wyników stwierdzono, że rury ożebrowane spawane laserowo są wykonanae prawidowo, a w złączach spawanych nie ujawniono niezgodności

Purified chick embryo cell rabies vaccine: economical multisite intradermal regimen for post-exposure prophylaxis.

The standard six-dose intramuscular (i.m.) rabies post-exposure vaccine regimen using a new purified chick embryo cell (PCEC) vaccine was compared with two economical multisite intradermal (i.d.) PCEC regimens, a multisite i.m. PCEC schedule and a subcutaneous regimen using a suckling mouse brain (SMB) rabies vaccine manufactured in Thailand. The neutralizing antibody results for the four-site and eight-site i.d. and the standard i.m. PCEC regimens were similar over 3 months. A three-site i.m. PCEC regimen had no advantage. The SMB vaccine gave the lowest antibody levels. Human rabies immune globulin therapy significantly increased the GMT of all groups on day 7, unlike equine antirabies serum (EARS). Both antisera suppressed antibody responses to PCEC on days 14 and 28. Three generalized reactions probably related to EARS were the only serious side effects. An eight-site i.d. PCEC vaccine regimen proved as immunogenic as the routine i.m. schedule and, if implemented as post-exposure prophylaxis, would be the cheapest widely available tissue culture vaccine regimen. The protective efficiency should now be tested in patients bitten by rabid animals