Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba)

Yellow mustard (Sinapis alba) has a sporophytic self-incompatibility reproduction system. Genetically stable self-incompatible (SI) and self-compatible (SC) inbred lines have recently been developed in this crop. Understanding the S haplotype of different inbred lines and the inheritance of the self-(in)compatibility (SI/SC) trait is very important for breeding purposes. In this study, we used the S-locus gene-specific primers in Brassica rapa and Brassica oleracea to clone yellow mustard S-locus genes of SI lines Y514 and Y1130 and SC lines Y1499 and Y1501. The PCR amplification results and DNA sequences of the S-locus genes revealed that Y514 carried the class I S haplotype, while Y1130, Y1499, and Y1501 had the class II S haplotype. The results of our genetic studies indicated that self-incompatibility was dominant over self-compatibility and controlled by a one-gene locus in the two crosses of Y514 × Y1499 and Y1130 × Y1501. Of the five S-locus gene polymorphic primer pairs, Sal-SLGI and Sal-SRKI each generated one dominant marker for the SI phenotype of Y514; Sal-SLGII and Sal-SRKII produced dominant marker(s) for the SC phenotype of Y1501 and Y1499; Sal-SP11II generated one dominant marker for Y1130. These markers co-segregated with the SI/SC phenotype in the F(2) populations of the two crosses. In addition, co-dominant markers were developed by mixing the two polymorphic primer pairs specific for each parent in the multiplex PCR, which allowed zygosity to be determined in the F(2) populations. The SI/SC allele-specific markers have proven to be very useful for the selection of the desirable SC genotypes in our yellow mustard breeding program. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-013-9943-8) contains supplementary material, which is available to authorized users

AAC Oriental 200 oriental mustard

AAC Oriental 200 is a doubled-haploid (DH) line. It was produced via microspore culture from the F1 hybrid plants resulting from a cross between the oriental mustard varieties Cutlass and Forge. AAC Oriental 200 has a higher (6.5%) yield than the check variety Cutlass, and similar levels of blackleg and white rust resistance as Cutlass. AAC Oriental 200 is well adapted to all mustard growing areas of western Canada.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

AAC Brown 18 brown mustard

AAC Brown 18 is the first brown mustard (Brassica juncea L.) hybrid variety developed using our improved Ogura cms hybrid system at Agriculture and Agri-Food Canada – Saskatoon Research and Development Centre (AAFC-SRDC). AAC Brown 18 has significantly higher (24%) yield than the check variety Centennial Brown. It is resistant to white rust race 2a whereas Centennial Brown is susceptible to race 2a. AAC Brown 18 is well adapted to all mustard growing areas of western Canada.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Genetic Variation in Morphology, Seed Quality and Self-(in)Compatibility among the Inbred Lines Developed from a Population Variety in Outcrossing Yellow Mustard (Sinapis alba)

Yellow mustard (Sinapis alba L.) has been grown as an important source of condiment for the spice trade in the world. It is an obligate outcrossing species due to its sporophytic self-incompatibility (SI). To utilize heterosis for yield potential, we have attempted to develop elite component inbred lines for producing high-yielding synthetic varieties for this crop. The open-pollinated variety Andante was used as the initial population. To circumvent the SI barrier, bud-pollination for selfing was performed on the selected initial (S0) plants. Various types of inbreeding depression were observed in the S1 generation. Elite inbred lines tolerant to inbreeding were produced by purging the deleterious alleles in each inbred generation. Self-compatible (SC) lines were developed for the first time in this species. There were three types of erucic variants (high: 49.9%, median: 23.9% and low: 1.4%), three types of linolenic variants (high: 18.5%, median: 13.8% and low: 3.8%) and two types of mucliage variants (high: 164.0 cS*mL/g and low: 12.0 cS*mL/g) among the developed inbred lines. These variants are being used to investigate the genetic and molecular mechanism underpinning the phenotypic variation of the seed oil profile and SI/SC traits in yellow mustard

Detection and Molecular Characterization of Two <i>FAD3</i> Genes Controlling Linolenic Acid Content and Development of Allele-Specific Markers in Yellow Mustard (<i>Sinapis alba</i>)

<div><p>Development of yellow mustard (<i>Sinapis alba</i> L.) with superior quality traits (low erucic and linolenic acid contents, and low glucosinolate content) can make this species as a potential oilseed crop. We have recently isolated three inbred lines Y1127, Y514 and Y1035 with low (3.8%), medium (12.3%) and high (20.8%) linolenic acid (C18∶3) content, respectively, in this species. Inheritance studies detected two fatty acid desaturase 3 (<i>FAD3</i>) gene loci controlling the variation of C18∶3 content. QTL mapping revealed that the two <i>FAD3</i> gene loci responsible for 73.0% and 23.4% of the total variation and were located on the linkage groups Sal02 and Sal10, respectively. The <i>FAD3</i> gene on Sal02 was referred to as <i>SalFAD3.LA1</i> and that on Sal10 as <i>SalFAD3.LA2</i>. The dominant and recessive alleles were designated as <i>LA¹</i> and <i>la¹</i> for <i>SalFAD3.LA1</i>, and <i>LA²</i> and <i>la²</i> for <i>SalFAD3.LA2</i>. Cloning and alignment of the coding and genomic DNA sequences revealed that the <i>SalFAD3.LA1</i> and <i>SalFAD3.LA2</i> genes each contained 8 exons and 7 introns. <i>LA¹</i> had a coding DNA sequence (CDS) of 1143 bp encoding a polypeptide of 380 amino acids, whereas <i>la¹</i> was a loss-of-function allele due to an insertion of 584 bp in exon 3. Both <i>LA²</i> and <i>la²</i> had a CDS of 1152 bp encoding a polypeptide of 383 amino acids. Allele-specific markers for <i>LA¹</i>, <i>la¹</i>, <i>LA²</i> and <i>la²</i> co-segregated with the C18∶3 content in the F₂ populations and will be useful for improving fatty acid composition through marker assisted selection in yellow mustard breeding.</p></div