Factorial Economic Planning Applied to Agricultural Experimentation

Innovation may be limited by the scarceness of resources, such as financial, homogeneous area, skilled labor or other research needs, for example the difficulty in experimental control of large areas in the field. In research areas such as chemistry and physics, designs are used in such a way that when compared to the agronomic designs, they result in a reduced number of experimental units, which in this work are called economic designs. Thus, the objective of this study was to identify significant factors and effects (p-valu

ARES. III. Unveiling the Two Faces of KELT-7 b with HST WFC3*

We present the analysis of the hot-Jupiter KELT-7 b using transmission and emission spectroscopy from the Hubble Space Telescope, both taken with the Wide Field Camera 3. Our study uncovers a rich transmission spectrum that is consistent with a cloud-free atmosphere and suggests the presence of H_{2}O and H^{−}. In contrast, the extracted emission spectrum does not contain strong absorption features and, although it is not consistent with a simple blackbody, it can be explained by a varying temperature–pressure profile, collision induced absorption, and H^{-}. KELT-7 b had also been studied with other space-based instruments and we explore the effects of introducing these additional data sets. Further observations with Hubble, or the next generation of space-based telescopes, are needed to allow for the optical opacity source in transmission to be confirmed and for molecular features to be disentangled in emission

Structural Basis for the Aminoacid Composition of Proteins from Halophilic Archea

In order to survive in highly saline environments, proteins from halophilic archea have evolved with biased amino acid compositions that have the capacity to reduce contacts with the solvent

Single-cell analysis reveals individual spore responses to simulated space vacuum

Outer space is a challenging environment for all forms of life, and dormant spores of bacteria have been frequently used to study the survival of terrestrial life in a space journey. Previous work showed that outer space vacuum alone can kill bacterial spores. However, the responses and mechanisms of resistance of individual spores to space vacuum are unclear. Here, we examined spores’ molecular changes under simulated space vacuum (~10−5 Pa) using micro-Raman spectroscopy and found that this vacuum did not cause significant denaturation of spore protein. Then, live-cell microscopy was developed to investigate the temporal events during germination, outgrowth, and growth of individual Bacillus spores. The results showed that after exposure to simulated space vacuum for 10 days, viability of spores of two Bacillus species was reduced up to 35%, but all spores retained their large Ca2 +-dipicolinic acid depot. Some of the killed spores did not germinate, and the remaining germinated but did not proceed to vegetative growth. The vacuum treatment slowed spore germination, and changed average times of all major germination events. In addition, viable vacuum-treated spores exhibited much greater sensitivity than untreated spores to dry heat and hyperosmotic stress. Among spores’ resistance mechanisms to high vacuum, DNA-protective α/β−type small acid-soluble proteins, and non- homologous end joining and base excision repair of DNA played the most important roles, especially against multiple cycles of vacuum treatment. Overall, these results give new insight into individual spore’s responses to space vacuum and provide new techniques for microorganism analysis at the single-cell level

Characterising a World Within the Hot Neptune Desert: Transit Observations of LTT 9779 b with HST WFC3

We present an atmospheric analysis of LTT 9779 b, a rare planet situated in the hot Neptune desert, that has been observed with HST WFC3 G102 and G141. The combined transmission spectrum, which covers 0.8 - 1.6 $\mu$m, shows a gradual increase in transit depth with wavelength. Our preferred atmospheric model shows evidence for H$_{\rm 2}$O, CO$_{\rm 2}$ and FeH with a significance of 3.1 $\sigma$, 2.4 $\sigma$ and 2.1 $\sigma$, respectively. In an attempt to constrain the rate of atmospheric escape for this planet, we search for the 1.083 $\mu$m Helium line in the G102 data but find no evidence of excess absorption that would indicate an escaping atmosphere using this tracer. We refine the orbital ephemerides of LTT 9779 b using our HST data and observations from TESS, searching for evidence of orbital decay or apsidal precession, which is not found. The phase-curve observation of LTT 9779 b with JWST NIRISS should provide deeper insights into the atmosphere of this planet and the expected atmospheric escape might be detected with further observations concentrated on other tracers such as Lyman $\alpha$.Comment: Accepted for publication in A

Characterizing a World Within the Hot-Neptune Desert: Transit Observations of LTT 9779 b with the Hubble Space Telescope/WFC3

We present an atmospheric analysis of LTT 9779 b, a rare planet situated in the hot-Neptune desert, that has been observed with Hubble Space Telescope (HST)/WFC3 with G102 and G141. The combined transmission spectrum, which covers 0.8–1.6 μm, shows a gradual increase in transit depth with wavelength. Our preferred atmospheric model shows evidence for H2O, CO2, and FeH with a significance of 3.1σ, 2.4σ, and 2.1σ, respectively. In an attempt to constrain the rate of atmospheric escape for this planet, we search for the 1.083 μm helium line in the G102 data but find no evidence of excess absorption that would indicate an escaping atmosphere using this tracer. We refine the orbital ephemerides of LTT 9779 b using our HST data and observations from TESS, searching for evidence of orbital decay or apsidal precession, which are not found. The phase-curve observation of LTT 9779 b with JWST NIRISS should provide deeper insights into the atmosphere of this planet and the expected atmospheric escape might be detected with further observations concentrated on other tracers such as Lyα