34 research outputs found

    A Fault-Tolerant Scheduling Algorithm using Hybrid Overloading Technology for Dynamic Grouping based Multiprocessor Systems

    Get PDF
    In order to extend the application area of fault-tolerant scheduling algorithm based on hybrid overloading for multiprocessor and increase the fault-tolerant number of processors, we propose a new fault-tolerant scheduling algorithm, which is based on hybrid overloading and dynamic grouping for multiprocessor by combining logic grouping strategy for processors in primary backup overloading and backup backup overloading.This algorithm presents the formalization of the dynamic grouping for processors in fault-tolerant scheduling based on hybrid overloading and enlarges the task number included in overloading task link. In the process of fault-tolerant scheduling the processors are dynamically divided into some groups based on overloading task link, so as to keep good scheduling success ratio and enhance the fault-tolerant performance of processors. Both theoretical analysis and simulation experiment prove this algorithm’s effectiveness respectively

    Single longitudinal mode narrow linewidth thulium-doped fiber laser based on an eye-shaped dual-ring filter

    Get PDF
    A single-longitudinal-mode (SLM) thulium-doped fiber laser, based on an eye-shaped passive dual-ring filter, is designed and constructed. The eye-shaped passive compound cavity consisting of four couplers is used to increase the longitudinal mode spacing, and its performance is numerically analyzed in detail. A homemade uniform fiber Bragg grating serves as a wavelength selection device and a saturable absorber is used to further suppress the intense longitudinal mode competition in the laser cavity, ensuring the single-longitudinal-mode output. The experimental results demonstrate a laser output with a center wavelength of 2,049.85 nm and an optical signal-to-noise ratio of 63 dB. Moreover, the power fluctuation is less than 0.6 dB, and the center wavelength fluctuation is less than 0.03 nm over a continuous measurement period of 60 min, demonstrating an excellent stability. The laser linewidth is measured using an unbalanced Michelson interferometer and β-separation line method, resulting in a linewidth of 11.22 kHz

    Comparative Proteomic and Morphological Change Analyses of Staphylococcus aureus During Resuscitation From Prolonged Freezing

    No full text
    When frozen, Staphylococcus aureus survives in a sublethally injured state. However, S. aureus can recover at a suitable temperature, which poses a threat to food safety. To elucidate the resuscitation mechanism of freezing survived S. aureus, we used cells stored at -18°C for 90 days as controls. After resuscitating the survived cells at 37°C, the viable cell numbers were determined on tryptic soy agar with 0.6% yeast extract (TSAYE), and the non-injured-cell numbers were determined on TSAYE supplemented with 10% NaCl. The results showed that the total viable cell number did not increase within the first 3 h of resuscitation, but the osmotic regulation ability of freezing survived cells gradually recovered to the level of healthy cells, which was evidenced by the lack of difference between the two samples seen by differential cell enumeration. Scanning electron microscopy (SEM) showed that, compared to late exponential stage cells, some frozen survived cells underwent splitting and cell lysis due to deep distortion and membrane rupture. Transmission electron microscopy (TEM) showed that, in most of the frozen survived cells, the nucleoids (low electronic density area) were loose, and the cytoplasmic matrices (high electronic density area) were sparse. Additionally, a gap was seen to form between the cytoplasmic membranes and the cell walls in the frozen survived cells. The morphological changes were restored when the survived cells were resuscitated at 37°C. We also analyzed the differential proteome after resuscitation using non-labeled high-performance liquid chromatography–mass spectrometry (HPLC-MS). The results showed that, compared with freezing survived S. aureus cells, the cells resuscitated for 1 h had 45 upregulated and 73 downregulated proteins. The differentially expressed proteins were functionally categorized by gene ontology enrichment, KEGG pathway, and STRING analyses. Cell membrane synthesis-related proteins, oxidative stress resistance-related proteins, metabolism-related proteins, and virulence factors exhibited distinct expression patterns during resuscitation. These findings have implications in the understanding of the resuscitation mechanism of freezing survived S. aureus, which may facilitate the development of novel technologies for improved detection and control of foodborne pathogens in frozen food

    Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide

    No full text
    Real-time polymerase chain reaction (PCR) allows rapid detection of Salmonella in frozen dairy products, but it might cause a false positive detection result because it might amplify DNA from dead target cells as well. In this study, Salmonella-free frozen ice cream was initially inoculated with heat-killed Salmonella Typhimurium cells and stored at −18°C. Bacterial DNA extracted from the sample was amplified using TaqMan probe-based real-time PCR targeting the invA gene. Our results indicated that DNA from the dead cells remained stable in frozen ice cream for at least 20 days, and could produce fluorescence signal for real-time PCR as well. To overcome this limitation, propidium monoazide (PMA) was combined with real-time PCR. PMA treatment can effectively prevent PCR amplification from heat-killed Salmonella cells in frozen ice cream. The PMA real-time PCR assay can selectively detect viable Salmonella at as low as 103 CFU/mL. Combining 18 hours of pre-enrichment with the assay allows for the detection of viable Salmonella at 100 CFU/mL and avoiding the false-positive result of dead cells. The PMA real-time PCR assay provides an alternative specifically for detection of viable Salmonella in ice cream. However, when the PMA real-time PCR assay was evaluated in ice cream subjected to frozen storage, it obviously underestimated the contamination situation of viable Salmonella, which might lead to a false negative result. According to this result, the use of enrichment prior to PMA real-time PCR analysis remains as the more appropriate approach

    a hybrid fault-tolerant scheduling algorithm for multiprocessor in space environment

    No full text
    In order to improve the efficiency of fault-tolerant scheduling of hybrid real-time tasks in circumstance of space application, a hybrid fault-tolerant scheduling algorithm for multiprocessor in space environment is proposed. In this algorithm, a grouping based Best-Fit assignment strategy in the periodic task scheduling is used to obtain more compact scheduling result by use of a little increasing computation time, so that the number of processors and the task execution time required by the algorithm are saved. In addition, a time slice based approach is used to finish the dynamic scheduling of aperiodic tasks, thus improving the efficiency of fault-tolerant scheduling of hybrid real-time tasks and making the algorithm more suitable for the task processing in complicated space environment. It is shown from the simulation that this algorithm has important effect on improving the performance of fault-tolerant scheduling of hybrid real-time tasks.In order to improve the efficiency of fault-tolerant scheduling of hybrid real-time tasks in circumstance of space application, a hybrid fault-tolerant scheduling algorithm for multiprocessor in space environment is proposed. In this algorithm, a grouping based Best-Fit assignment strategy in the periodic task scheduling is used to obtain more compact scheduling result by use of a little increasing computation time, so that the number of processors and the task execution time required by the algorithm are saved. In addition, a time slice based approach is used to finish the dynamic scheduling of aperiodic tasks, thus improving the efficiency of fault-tolerant scheduling of hybrid real-time tasks and making the algorithm more suitable for the task processing in complicated space environment. It is shown from the simulation that this algorithm has important effect on improving the performance of fault-tolerant scheduling of hybrid real-time tasks

    Exogenous abscisic acid inhibits the water-loss of postharvest romaine lettuce during storage by inducing stomatal closure

    No full text
    Abstract Postharvest lettuce often lose water, thus affecting both its market value and consumer acceptance. However, the mechanism of the water-loss is still waiting well exploration. The aim of the present study was to investigate the effect of a foliar application of ABA on the fresh weight-loss and the chlorophyll content of postharvest lettuce as well as its association with the regulation of stomata. The present data demonstrated that exogenously application of ABA, in a concentration range of 0 to 100 µM, significantly lowered the fresh weight-loss of postharvest lettuce. ABA also delayed chlorophyll reduction during ambient storage, but this protective effect was ABA concentration-dependent. Among the tested ABA concentrations, 50 µM or lower ABA produced an inhibition effect on chlorophyll degradation in postharvest lettuce leaves. The results demonstrated that the exogenous ABA treatment can obviously reduce the transpiration rate of lettuce leaves by promoting the stomatal closure of postharvest lettuce, therefore eventually delay fresh weight-loss. The present study primarily showed that the application of exogenous ABA, which originated from a naturally-produced phytohormone, has a great potential in retaining the freshness of postharvest lettuce that is stored in an ambient condition, although possible practical application still need to be further evaluated
    corecore