4 research outputs found
High-throughput screening of encapsulated islets using wide-field lens-free on-chip imaging
Islet microencapsulation is a promising solution to diabetes treatment, but
its quality control based on manual microscopic inspection is extremely
low-throughput, highly variable and laborious. This study presents a
high-throughput islet-encapsulation quality screening system based on lens-free
on-chip imaging with a wide field-of-view of 18.15 cm^2, which is more than 100
times larger than that of a lens-based optical microscope, enabling it to image
and analyze ~8,000 microcapsules in a single frame. Custom-written image
reconstruction and processing software provides the user with clinically
important information, such as microcapsule count, size, intactness, and
information on whether each capsule contains an islet. This high-throughput and
cost-effective platform can be useful for researchers to develop better
encapsulation protocols as well as perform quality control prior to
transplantation
Design of Safety Evaluation and Risk Traceability System for Agricultural Product Quality
The quality and safety of agricultural products involve a variety of risk factors, a large amount of risk information data, and multiple circulation and disposal processes, making it difficult to accurately trace the source of risks. To achieve precise traceability of agricultural product quality and safety, the monitoring and supervision level of agricultural product traceability systems needs to be improved. In this paper, the agricultural product quality- and safety-related detection data are taken as the research object, the agricultural product supply chain and business process are sorted out, the agricultural product risk factors under the influence of heavy metals are analyzed, and the agricultural product quality and safety risk evaluation model is established. The agricultural product risk traceability system was designed based on the six-layer architecture of the equipment layer, data layer, service layer, application layer, display layer, and user layer. Combined with the agricultural product quality and safety risk evaluation model, the system data were stored and modified by using the MySQL relational database, and the agricultural product risk traceability system was developed, which realized the accurate traceability of the risk link of the quality and safety of agricultural products. The research results of this paper provide an effective information means for the quality and safety supervision of agricultural products, and provide a technical reference for the development of a traceability system of risk factors related to agricultural products, which has good practical significance
Computational Portable Microscopes for Point-of-Care-Test and Tele-Diagnosis
In bio-medical mobile workstations, e.g., the prevention of epidemic viruses/bacteria, outdoor field medical treatment and bio-chemical pollution monitoring, the conventional bench-top microscopic imaging equipment is limited. The comprehensive multi-mode (bright/dark field imaging, fluorescence excitation imaging, polarized light imaging, and differential interference microscopy imaging, etc.) biomedical microscopy imaging systems are generally large in size and expensive. They also require professional operation, which means high labor-cost, money-cost and time-cost. These characteristics prevent them from being applied in bio-medical mobile workstations. The bio-medical mobile workstations need microscopy systems which are inexpensive and able to handle fast, timely and large-scale deployment. The development of lightweight, low-cost and portable microscopic imaging devices can meet these demands. Presently, for the increasing needs of point-of-care-test and tele-diagnosis, high-performance computational portable microscopes are widely developed. Bluetooth modules, WLAN modules and 3G/4G/5G modules generally feature very small sizes and low prices. And industrial imaging lens, microscopy objective lens, and CMOS/CCD photoelectric image sensors are also available in small sizes and at low prices. Here we review and discuss these typical computational, portable and low-cost microscopes by refined specifications and schematics, from the aspect of optics, electronic, algorithms principle and typical bio-medical applications