93 research outputs found
Transient Alteration of Cellular Redox Buffering before Irradiation Triggers Apoptosis in Head and Neck Carcinoma Stem and Non-Stem Cells
Background: Head and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy owing to intrinsic radioresistance and lack of induction of apoptosis. The major focus of this work was to design a transient glutathione depleting strategy during the course of irradiation of HNSCC in order to overcome their radioresistance associated with redox adaptation. Methodology/Principal Findings: Treatment of SQ20B cells with dimethylfumarate (DMF), a GSH-depleting agent, and L-Buthionine sulfoximine (BSO), an inhibitor of GSH biosynthesis 4 h before a 10 Gy irradiation led to the lowering of the endogenous GSH content to less than 10 % of that in control cells and to the triggering of radiation-induced apoptotic cell death. The sequence of biochemical events after GSH depletion and irradiation included ASK-1 followed by JNK activation which resulted in the triggering of the intrinsic apoptotic pathway through Bax translocation to mitochondria. Conclusions: This transient GSH depletion also triggered radiation-induced cell death in SQ20B stem cells, a key event to overcome locoregional recurrence of HNSCC. Finally, our in vivo data highlight the relevance for further clinical trials o
Effects of tumour acidification with glucose+MIBG on the spontaneous metastatic potential of two murine cell lines
Interactions of the carcinogen 4-nitroquinoline 1-oxide with the non protein thiols of mammalian cells.
The use of the oxidant \u27diamide\u27 for studying the non-mitochondrial reducing capacity of ehrlich ascites tumor cells.
Coenzyme interactions: proton magnetic resonance study of molecular complexes of thiamine and indole derivatives.
Effect of insulin-glucose altered metabolic states on the response of ehrlich ascites tumors to ionizing radiation. Abstr.
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