Isolation, cloning and characterization of phlB gene from an Indian strain of Gram negative soil bacteria Pseudomonas fluorescens

412-419Phloroglucinol (PHL) or 2,4-diacetylphloroglucinol (DAPG) is a polyketide compound that exhibits broad spectrum anti-bacterial and antifungal properties against soil-borne plant pathogens. It is produced by gram negative bacterium Pseudomonas (fluorescent Pseudomonad) which renders them a potential biological control agent. The process of PHL production is accomplished by a well coordinated activity of four (phlABCD) genes arranged in an operon. All four genes are essential and indispensable for biosynthesis of DAPG from precursor malonyl CoA. Here, we report cloning and analysis of phlB gene from an Indian strain of Pseudomonas fluorescens. Further, we predicted the structure of PHLB protein and analyzed its intrinsic details. Stereo-chemical properties were analyzed by Ramachandran plot and secondary structure was retrieved by PDB sum. Our study provides insight into the structure of PHLB protein and revealed that though Pseudomonas PHLB lacks conserved motifs and domains it is indispensable for monoacetyl phloroglucinol (MAPG) synthesis

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Not AvailableBacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating disease and limiting factor for rice productivity worldwide. Since BLB pathogens are diffi cult to manage through chemical and other available cultural practices, development of host plant resistance is the most effective means of disease management. So far survey for resistance donor at molecular level in rice is scanty and the landraces of Gujarat state of India have received little attention. Therefore, a molecular screening was conducted to identify the presence of major BLB resistance genes Xa4, xa5 and Xa21 in landraces and local cultivars of Gujarat. In order to confi rm the presence of BLB resistance genes, 25 genotypes were screened using PCR analysis. 10 genotypes (IR20, IR64, IR72, NWGR1095, NWGR2014, IET14726, GR7, GR102, Pankhali 203 and Ratna) were found to be Xa4 positive. In contrast, only IET18483 genotype was found to be Xa21 positive. Interestingly, not a single genotype was found to be positive for xa5 BLB resistance gene. The status of BLB resistance genes in landraces and cultivars of Gujarat will help design BLB-resistance breeding programs.Not Availabl