Adjusted odds ratio (ORs) along with 95% CI for various aspects of knowledge on cervical cancer and HPV among college students.

<p>Adjusted odds ratio (ORs) along with 95% CI for various aspects of knowledge on cervical cancer and HPV among college students.</p

Questionnaire employed for awareness about cervical cancer and HPV.

<p>Questionnaire employed for awareness about cervical cancer and HPV.</p

Knowledge about cervical cancer and HPV among college students based on their sex.

<p>Knowledge about cervical cancer and HPV among college students based on their sex.</p

Receiver operating characteristic curve (ROC) analysis for the set of 6 microRNAs for total breast cancer tissues and sera.

<p>Receiver operating characteristic curve (ROC) analysis for the set of 6 microRNAs for total breast cancer tissues and sera.</p

Pearson correlation coefficient of miRNAs in TNBC tissue and sera.

<p>Pearson correlation scatter plot of the fold-change expression level of miRNAs in tissue and sera from triple negative breast cancer patients which predicts a good correlation with tumor grade. (A) miR-21; (B) miR-221; (C) miR-210 and (D) Let-7a has showed significant positive correlation with respect to tumor grade. (E) miR-195; (F) miR-145 showed significant negative correlation or inverse correlation with increasing tumor grade.</p

The expression level of six specific miRNAs in serum specimens from TNBC and other subtypes of breast cancer, benign breast cancer and controls.

<p>(A) Expression level of selected miRNAs (miR-21, miR-221, miR-210, miR-195, miR-145 and Let-7a) in total serum samples from breast cancer patients and healthy individuals. (B) Expression profiles of miR-21, miR-221, miR-210, miR-195, miR-145 and Let-7a in TNBC patients and other hormonal subtypes (ER+/PR+/Her2neu+), (ER-/PR+/Her2neu+) and (ER-/PR-/Her2neu+) in sera from breast cancer patients along with healthy individuals. (C) Expression level of miR-21, miR-221, miR-210, miR-195, miR-145 and Let-7a in sera of fibroadenoma and different tumor grades of breast cancer and healthy controls. (D) Comparison of <b>e</b>xpression level of miR-21, miR-221, miR-210, miR-195, miR-145 and Let-7a in TNBC tissue, sera and cell lines (MCF-7 and MDA-MB-231) with normal mammary epithelial cell line MCF-10A.</p

Identification of Specific miRNA Signature in Paired Sera and Tissue Samples of Indian Women with Triple Negative Breast Cancer

<div><p>Of several subtypes of breast cancer, triple negative breast cancer (TNBC) is a highly aggressive tumor that lacks expression of hormone receptors for estrogen, progesterone and human epidermal growth factor receptor 2 and shows a worst prognosis. The small noncoding RNAs (miRNAs) considered as master regulator of gene expression play a key role in cancer initiation, progression and drug resistance and have emerged as attractive molecular biomarkers for diagnosis, prognosis and treatment targets in cancer. We have done expression profiling of selected miRNAs in paired serum and tissue samples of TNBC patients and corresponding cell lines and compared with that of other subtypes, in order to identify novel serum miRNA biomarkers for early detection and progression of TNBC. A total of 85 paired tumor tissues and sera with an equal number of adjacent normal tissue margins and normal sera from age matched healthy women including tissue and sera samples from 15 benign fibroadenomas were employed for the study. We report for the first time an extremely high prevalence (73.9%) of TNBC in premenopausal women below 35 years of age and a significant altered expression of a panel of three specific oncogenic miRNAs- miR-21, miR-221, miR-210, and three tumor suppressor miRNAs- miR-195, miR-145 and Let-7a in both tissues and corresponding sera of TNBC patients when compared with triple positive breast cancer (TPBC) patients. While miR-21, miR-221 and miR-210 showed significant over-expression, miR-195 and miR-145 were downregulated and well correlated with various clinicopathological and demographic risk factors, tumor grade, clinical stage and hormone receptor status. Interestingly, despite being a known tumor suppressor, Let-7a showed a significant overexpression in TNBCs. It is suggested that this panel of six miRNA signature may serve as a minimally invasive biomarker for an early detection of TNBC patients.</p></div

Clinicoepidemiological characteristics of TNBC and other subtypes of breast cancer patients.

<p>Clinicoepidemiological characteristics of TNBC and other subtypes of breast cancer patients.</p

MicroRNA expression level in breast cancer cell lines.

<p>MicroRNA expression level in breast cancer cell lines.</p

Receiver operating characteristic curve analysis of specific miRNAs in TNBC patients.

<p>Receiver operating characteristic curve of the diagnostic potential of the individual serum miRNAs. A (miR-21); B (miR-221); C (miR-210); D (miR-195); E (miR-145); F (Let-7a); (G) comparative ROC of all six miRNAs (miR-21; miR-221; miR-210; miR-195, miR-145 and Let-7a); (H) combined ROC of three miRs (miR-21; miR-221 and miR-210); (I) combined ROC of two downregulated miRNAs (miR-195 and miR-145). This predicts the diagnostic accuracy and expression level of miRNA signature that are able to discriminate between patients and healthy controls in the sera of triple negative breast cancer.</p