Analysis of the association of HBV sub-genotypes with biochemical parameters.

<p>Median (A) ALT (IU/liter) and (B) bilirubin (IU/liter) levels in patients vary with circulating HBV sub-genotypes. Only significant differences (p<0.05) have been marked in the figure.</p

Frequencies of some principal mutations in the HBV genome compared in acute and chronic Hepatitis B patients.

<p>Figure depicts some signature mutations in the surface gene (S), basal core promoter/pre-core regions (BCP/PC) and X gene.</p

Phylogenetic analysis of HBV isolates from Eastern India with acute infection created using the neighbour-joining method.

<p>Phylogenetic tree was constructed from surface gene sequences of this study (denoted by AEI) along with reference sequences derived from GenBank (denoted by accession numbers).</p

Comparison of nucleotide diversity in acute Vs chronic infection.

<p>Nucleotide diversity in the (A) surface gene, (B) BCP/PC region and (C) X gene has been compared. The nucleotide sequences of the chronic sequences were compared to that of the acute sequences using Shannon’s Entropy. The blue lines indicated the specific positions where changes were noted. The red lines indicate the positions where the frequency of substitution between acute and chronic sequences was significantly different. (D) The average ΔH (average difference in entropy between chronic and acute infection) was highest in the BCP region of the HBV genome. (E) Calculation of genetic diversity at the genotype level showing that the BCP/PC region of genotype C isolates exhibit highest average ΔH.</p

Association of HBV occurrence with the modes of transmission, patients’ age groups and HBV genotypes among samples included in the study.

<p>(A) Sexual promiscuity is the major mode by which HBV is transmitted in the study population. (B) Occurrence of HBV is highest in the age bracket between 15 to 30 years. (C) HBV/D2 is the dominant sub-genotype and is associated majorly with sexual promiscuity. (D) Occurrence of HBV/D2 is highest in the age bracket between 15 to 30 years.</p

Distribution of HBV genotypes and sub-genotypes among acute and chronic patients of eastern India.

<p>(A) Difference in HBV genotype distribution among acute and chronic patients. (B) Difference in the distribution of HBV sub-genotypes under genotype D among acute and chronic patients. Only significant results (*p<0.05, **P<0.01) have been marked in the figure.</p

Demographic, biochemical and virological characteristics of acute and chronic subjects from eastern India.

<p>Statistically significant values are denoted by *(p<0.05) or **(p<0.001).</p