Evaluation of anti-inflammatory activity of Boswellia serrata on carrageenan induced paw edema in albino Wistar rats

Background: Inflammation is a response of the immune system, guarding the individual against infection. It is a major burning problem worldwide and billions of individuals are affected. Moreover administration of current anti-inflammatory drugs is often associated with severe side effects. Hence alternative therapeutic modules are necessitated. Now a day’s herbal medicines are using due to their high efficacy and harmless to cure the diseases. In traditional medicine Boswellia serrata (B. serrata) has been widely used to treat various diseases which also include Inflammation. Till now the effect of B serrata on inflammation was not well understood. Hence In the present study we made an attempt to evaluate the anti-inflammatory activity of B. Serrata against carrageenan induced paw edema which is acute model of inflammation.Methods: Albino wistar rats were divided into five groups, group 1 treated with carrageenan (control) whereas group 2, 3, and 4 treated with different doses (50, 100, and 200 mg/kg/bw) of B. serrata along with carrageenan, respectively. Group 5 treated with standard drug (Indomethacin 10 mg/kg/bw). Carrageenan induced paw edema and histopathological study of paw were evaluated in all experimental rats.Results: The present study clearly demonstarted that carrageenan significantly increased paw edema and cellular infiltrates whereas B. serrata treated rats significantly decreased the paw edema and histopathological finding of cellular infiltrates and found to be greater at higher concentration i.e., 200 mg/kg/b/wt as compared to standard drug. Conclusions: The findings from the above study proves that B. serrata has high anti-inflammatory activity and supports its usage in traditional medicine as herbal anti-inflammatory medicine.

Bioflavonoid hesperidin possesses the anti-hyperglycemic and hypolipidemic property in STZ induced diabetic myocardial infarction (DMI) in male Wister rats

The aim of our study was to evaluate the hesperidin anti-hyperglycemic and hypo-lipidemic effects on diabetic myocardial infraction (DMI) rats by decreasing the blood glucose and blood cholesterol contents. The object of the study was to examine the 7groups of male Wistar rats, each group contains 6 rats, Group I (normal), Group II diabetic (control) and 5(experimental) groups, i.e., Group III (diabetic + hesperidin), Group IV (diabetic + Glibenclamide), Group V (ISO), Group VI (diabetic + ISO) and Group VII (diabetic + ISO + hesperidin). By that intake of hesperidin regulates the lipid and carbohydrate metabolism by decreasing the cholesterols in blood and plasma insulin by hyperlipidamic and anti-hyperglycemic activity. The rats turns to diabetic with single intraperitonial STZ injection (50 mg/kg BW), and from the second week the rats diet were switched to a high fat diet, i.e., cholesterol (40%), fat (50%), Protein (50%) turns to be hyperlipidamic rats and with Isoproterenol (ISO) single intraperitoneal injection by the (11 mg/kg BW) turns to DMI rats. The DMI rats fed with the hesperidin 100mg/kg BW for 4 weeks had significantly reduced the blood glucose (P < 0.05), total cholesterol (TC), Triglycerides, HDL, LDL and VLDL concentrations, when compared with experimental groups (p < 0.05). A significantly increased blood glucose and body weight was observed in hesperidin treated diabetic groups (P < 0.05) by comparing with the experimental groups. In conclusion, orally supplementation of hesperidin possesses a significant decrease in total blood lipid profiles and plasma insulin concentrations accompanied by the anti-hyperglycemic, hypo-lipidemic activity in DMI rats. Keywords: Hesperidin, Diabetic cardiomyopathy, Anti-hyperglycemic, Hypolipidemi

A study on antimicrobial and anticancer properties of Cissus quadrangulris using lung cancer cell line

Cissus quadrangularis plant from Vitaceae family, native in India. Many parts of this plant have medicinal values but most precious is stem of this plant. In past years number of studies reported their activities and secondary metabolites in Cissus quadrangularis plant and their pharmacological activities and uses in traditional medicine system. It is reported to possess excellent medicinal properties and potent fracture healing properties, antimicrobial, antiulcer, antioxidative, cholinergic activity and beneficial effect on cardiovascular diseases, possesses antiulcer and cytoprotective property in indomethacin-induced gastric mucosal injury. The aim of this study was to determine the qualitative phytochemical analysis, antimicrobial activity, cell viability and in vitro anticancer activity of a potential of Cissus quadrangularis stem extract against A549 human lung cancer cell line. The disc diffusion method was employed to determine the antimicrobial activity of Cissus quadrangularis stem extract and showed potential antibacterial and antifungal activity against various microorganisms. Results have shown that Stem methanolic extract induced a significant decrease of tumour cell viability. The cell viability assay clearly showed that the cells treated with Cissus quadrangularis methanolic extract has significantly reduced the lung cancer cell viability in a dose dependant manner. The stem methanolic extract was tested for the in vitro antiproliferative potential on A549 human lung cancer cell line using different concentrations, namely 1000, 62.5 and 7.8 µg/ml. We observed the IC50 dose at 65.2 μg/ml concentration. In cell culture A549 cells treated with Cissus quadrangularis stem methanolic extract in 24 h the cells growth is controlled

Calcium, zinc and vitamin E ameliorate cadmium-induced renal oxidative damage in albino Wistar rats

This study was aimed to examine the protective effects of supplementation with calcium + zinc (Ca + Zn) or vitamin E (Vit-E) on Cd-induced renal oxidative damage. Young albino Wistar rats (180 ± 10 g) (n = 6) control rats, Cd, Cd + Ca + Zn, and Cd + Vit-E experimental groups and the experimental period was 30 days. Rats were exposed to Cd (20 mg/kg body weight) alone treated as Cd treated group and the absence or presence of Ca + Zn (2 mg/kg each) or Vit-E (20 mg/kg body weight) supplementation treated as two separate groups. The activities of the stress marker enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and lipid peroxidase (LPx) were determined in renal mitochondrial fractions of experimental rats. We observed quantitative changes in SOD isoenzymatic patterns by non-denaturing PAGE analysis, and quantified band densities. These results showed that Cd exposure leads to decreases in SOD, CAT, GR, and GPx activities and a concomitant increase in LPx and GST activities. Ca + Zn and Vit-E administration with Cd significantly reversed Cd-induced perturbations in oxidative stress marker enzymes. However, Vit-E showed more inhibitory activity against Cd than did Ca + Zn, and it protected against Cd-induced nephrotoxicity. Keywords: Cadmium (Cd), Oxidative stress, Lipid peroxidation, Nephrotoxicity, PAGE analysi

Mutations in exons 10 and 11 of human glucokinase result in conformational variations in the active site of the structure contributing to poor substrate binding – explains hyperglycemia in type 2 diabetic patients

<div><p>Mutations in the glucokinase (GK) gene play a critical role in the establishment of type 2 diabetes. In our earlier study, R308K mutation in GK in a clinically proven type 2 diabetic patient showed, structural and functional variations that contributed immensely to the hyperglycemic condition. In the extension of this work, a cohort of 30 patients with established type 2 diabetic condition were chosen and the exons 10 and 11 of GK were PCR-amplified and sequenced. The sequence alignment showed A379S, D400Y, E300A, E395A, E395G, H380N, I348N, L301M, M298I, M381G, M402R, R308K, R394P, R397S, and S398R mutations in 12 different patients. The structural analysis of these mutated GKs, showed a variable number of β-α-β units, hairpins, β-bulges, strands, helices, helix–helix interactions, β-turns, and γ-turns along with the RMSD variations when compared to wild-type GK. Molecular modeling studies revealed that the substrate showed variable binding orientations and could not fit into the active site of these mutated structures; moreover, it was expelled out of the conformations. Therefore, these structural variations in GK due to mutations could be one of the strongest reasons for the hyperglycemic levels in these type 2 diabetic patients.</p></div