13 research outputs found
Alternative Biodefensive based on the Essential Oil from Allium sativum Encapsulated in PCL/Gelatin Nanoparticles
The goal of this paper was to develop a biodegradable system containing the essential oil from Allium sativum bulbs encapsulated in PCL/gelatin-based nanoparticles, as well as evaluate its efficiency to control Aedes aegypti Linn. larvae and Cerataphis lataniae Bois. aphids. The essential oil was analyzed by GC-FID and GC-MS, and six compounds were identified, representing 93.1% of the total oil. The major compounds were diallyl trisulfide (51.8%), diallyl disulfide (23.2%) and allyl methyl trisulfide (13.6%). The PCL/gelatin-based nanoparticles containing this essential oil exhibited encapsulation efficiency higher than 94%, average particle diameter around 200 nm and zeta potential values about -36 mV. The essential oil presented no antioxidant nor enzymatic activities, so its effectiveness might be explained by the presence of sulfur compounds. The release kinetics of the encapsulated essential oil confirmed the release mechanism by the Fick's Law. About 50% of the encapsulated essential oil was released after 1 h, and about 90% was released after 50 h. This behavior is interesting from the technological point of view since the nanoparticles released as much oil as possible in a short period of time and then the lethal dosages were maintained along the time. Nanoparticles containing the encapsulated essential oil was submitted to in vitro bioassays against A. aegypti and C. lataniae and showed 100% of mortality against larvae and aphids up to 24 h. In conclusion, the essential oil from A. sativum presented effectiveness to be applied in sustainable management of pests in greenhouses, as well as for larvicidal control
Escola organizada em ciclos: as representaçÔes sociais de professores considerados bem-sucedidos
Thermal Treatment and High-Intensity Ultrasound Processing to Evaluate the Chemical Profile and Antioxidant Activity of Amazon Fig Juices
The present paper evaluated the influence of heat treatment (HT) and high-intensity ultrasound (HIUS) on the chemical profile of the Amazon fig (Ficus subapiculata, Moraceae) juices. Antioxidant activity, quantification of carotenoids, total phenolic compounds (TPC), pH, titratable acidity, soluble solids, color and chemical profile (NMR) were evaluated. Treatments did not change the pH (3.4â3.5), titratable acidity (0.044â0.048%) and soluble solids (2.3â2.4 °Brix). The highest antioxidant activity (DPPH, ABTS) and TPC were presented by the HT-treated juice, which was equivalent to 1235 ± 11 ”M TE, 1440 ± 13 ”M TE and 312 ± 5 mg GAE mLâ1, respectively. The treatments influenced the color luminosity according to the L* and a* parameters, while the b* parameter showed no significant change. The L* parameter was elevated in all treated samples compared to the control sample. Analyzing the parameter a* f, it was verified that the sample with thermal treatment (HT) was different from the control sample, but presented similarity with the samples of the HIUS processes. The 1H NMR spectra of the juices showed similar chemical profiles in all treatments. The compounds α-glucose, ÎČ-glucose, fructose, citric, malic, quinic, and p-hydroxybenzoic acids were identified. The HT treatment presented higher efficiency to extract the antioxidant compounds from fig juices. The HIUS treatments with constant energy density also improved the tolerance of the antioxidant compounds, especially in conditions of higher potency and reduced time. Future studies will be devoted to carry out microbiological analysis and evaluate the stability of treated juices
Bioactivity of Licaria puchury-major Essential Oil Against Aedes aegypti , Tetranychus urticae and Cerataphis lataniae
The present study was carried out to evaluate an alternative controlling agent for greenhouse pests and the yellow fever mosquito larvae. The potential bioactivity of Licaria puchury-major (Mart.) Kosterm. (âpuxuriâ) was evaluated here against three most common pests in tropical and subtropical countries: Aedes aegypti Linn. Larvae, Tetranychus urticae Koch. mites and Cerataphis lataniae Boisd. aphids. The essential oil from seeds was analyzed by GC-FID and GC-MS. The major compounds were safrole (38.8%) and eucalyptol (21.7%). Phenylpropanes (51.7%) was the main group of compounds and oxygenated monoterpenes represented 28.8% of the total oil. The essential oil has shown no inhibition of acetylcholinesterase (AChE) in the tested concentrations. However, potential antioxidant activities were evaluated by different methods [DPPH: LD 50 = (27.8 ± 1.0) ”g/.mL; ABTS: (977.3 ± 25.2) ”M TEs/g (Trolox Equivalents); FRAP: (548.2 ± 29.0) ”M Fe(II)/g]. A significant larvicidal potential for 24 h of exposure was observed with LD 50 = 98.9 ”g/mL, being an indicative that the larval mortality may occur by ingestion or contact due to the no inhibition against AChE. Volatile phase effects were evaluated against T. urticae Koch. and C. lataniae Boisd. and LD 50 were found about 30.8 and 13.5 ”g/mL , respectively. These results are consistent with an octopaminergic effect , since some phenylpropanoids (such as Safrole, identified as the major compound in this work) can block octopamine, a multi-functional, naturally occurring biogenic amine. Then, this study clearly illustrated the efficacy of the investigated seeds, which encourages the development of a new potential natural controlling agent against these common pests due to the abundance of these seeds in the Amazon region and to the high essential oil yield
Theoretical Investigation of the Structural, Spectroscopic, Electronic, and Pharmacological Properties of 4-Nerolidylcathecol, an Important Bioactive Molecule
4-Nerolidylcatechol (4NRC), a secondary metabolite described as a potent antioxidant that presents anti-inflammatory, antimalarial, analgesic, and cytotoxic properties, has been receiving prominence in the catechol class. In this work, a theoretical DFT study of the vibrational, structural, and quantum properties of 4-nerolidylcatechol (4NRC) using the B3LYP/6-311G (2d,p) level is presented. The theoretical molecular geometry data were compared with the X-ray data of a similar molecule in the associated literature and a conformational study is presented, with the aim of providing a good comprehension of the 4NRC structural arrangement and stability. Also, HOMO-LUMO energy gap and natural bond orbitals (NBOs) were performed and discussed. The calculated UV spectrum showed similarity to the experimentally obtained data, with transitions assigned. The comparative IR studies revealed that intermolecular hydrogen bonds that stabilize dimeric forms are plausible and also allowed the assignment of several characteristic vibrations. Molecular docking calculations with DNA topoisomerase I-DNA complex (TOPO-I), glyceraldehyde 3-phospate dehydrogenase (GAPDH), and Plasmodium falciparum lactate dehydrogenase (PfLDH) showed binding free energies of â6.3, â6.5, and â7.6âkcal/mol, respectively, which indicates that 4NRC is a good competitive inhibitor for these enzymes
Ingestive behavior and physiological parameters of goats fed diets containing peanut cake from biodiesel
Spatial distribution of orchid bees in a rainforest/rubber agro-forest mosaic: habitat use or connectivity
Increased interregional virus exchange and nucleotide diversity outline the expansion of chikungunya virus in Brazil
Abstract The emergence and reemergence of mosquito-borne diseases in Brazil such as yellow fever, zika, chikungunya, and dengue have had serious impacts on public health. Concerns have been raised due to the rapid dissemination of the chikungunya virus across the country since its first detection in 2014 in Northeast Brazil. In this work, we carried out on-site training activities in genomic surveillance in partnership with the National Network of Public Health Laboratories that have led to the generation of 422 chikungunya virus genomes from 12 Brazilian states over the past two years (2021â2022), a period that has seen more than 312 thousand chikungunya fever cases reported in the country. These genomes increased the amount of available data and allowed a more comprehensive characterization of the dispersal dynamics of the chikungunya virus East-Central-South-African lineage in Brazil. Tree branching patterns revealed the emergence and expansion of two distinct subclades. Phylogeographic analysis indicated that the northeast region has been the leading hub of virus spread towards other regions. Increased frequency of Câ>âT transitions among the new genomes suggested that host restriction factors from the immune system such as ADAR and AID/APOBEC deaminases might be driving the genetic diversity of the chikungunya virus in Brazil
Genomic epidemiology reveals how restriction measures shaped the SARS-CoV-2 epidemic in Brazil
Abstract Brazil has experienced some of the highest numbers of COVID-19 infections and deaths globally and made Latin America a pandemic epicenter from May 2021. Although SARS-CoV-2 established sustained transmission in Brazil early in the pandemic, important gaps remain in our understanding of local virus transmission dynamics. Here, we describe the genomic epidemiology of SARS-CoV-2 using near-full genomes sampled from 27 Brazilian states and an adjacent country - Paraguay. We show that the early stage of the pandemic in Brazil was characterised by the co-circulation of multiple viral lineages, linked to multiple importations predominantly from Europe, and subsequently characterized by large local transmission clusters. As the epidemic progressed, the absence of effective restriction measures led to the local emergence and international spread of Variants of Concern (VOC) and under monitoring (VUM), including the Gamma (P.1) and Zeta (P.2) variants. In addition, we provide a preliminary genomic overview of the epidemic in Paraguay, showing evidence of importation from Brazil. These data reinforce the need for the implementation of widespread genomic surveillance in South America as a toolkit for pandemic monitoring and providing a means to follow the real-time spread of emerging SARS-CoV-2 variants with possible implications for public health and immunization strategies