Immunohistochemical Studies on S100 Reactivity in Chicks Experimentally Infected with Eimeria tenella I: The Localization of S100 Protein and Its Subunits alpha and beta in Stages of Eimeria tenella

The expression of the calcium-binding protein S100 and its subunits alpha and beta in the intracellular protozoon Eimeria tenella was investigated. For this purpose, anti-S100 whole molecule polyclonal antibody and anti-S100 alpha and anti-S100 alpha monoclonal antibodies were used on paraffin sections collected from the cecum, ileum, colon, rectum, and bursa of Fabricius of chicks experimentally infected with E. tenella. One hundred seventy-five 2- to 3-day-old specific pathogen-free chicks were used. The chicks were divided into experiment and control groups, to include 94 and 81 chicks, respectively. All the chicks in the experimental group received 10,000 spored oocytes in 0.5 ml inoculum in a single dose. The duration of the experiment was limited to 18 days. All 175 chicks were sacrificed by cervical dislocation between hours 1 and 432 following inoculation. The streptavidin-biotin complex method was used to detect the immunoreactivity of the antibodies used. The results revealed a positive immune reaction for the S100 whole molecule and for alpha and beta subunits in the schizonts, microgametes, macrogametes, and oocytes of the E. tenella in the cecum, ileum, colon, rectum, and bursa of Fabricius. These results show that both alpha and beta subunits of the S100 protein existed in all the endogenous phases of E. tenella. These proteins are likely to mediate the parasitic development of E. tenella within host cells

Accessory liver lobe anomaly in the abdominal region of a calf

In this observation, a mass weighing 450 g in weight and measuring 10x18x9 cm located in the umblical canal was surgically removed and macroscopical and microscopical examinationd were performed. It was concluded that the mass was liver tissue. The case was diagnosed as accessory liver lobe

A case of triphasic nephroblastoma with lung metastases in an Angora rabbit

Kidneys, liver, lung. and heart of a 1-year-old male Angora rabbit from a commercial facility were the material of this case. Macroscopically, an amorphous and elastic mass with cysts was observed on the left kidney. The mass weighed 875 grams and was 11 x 12x4.5 cm in dimensions. At the cross section, a tumor with lobular appearance, covering the pelvis and medulla of the kidney was observed, which distinguished from the atrophic kidney tissue via a distinctive capsule. Microscopically, blastemal. epithelial, and stromal areas containing polygonal neoplastic cells with pleomorphic hyperchromatic nuclei and with narrow cytoplasm were noticed. Primitive avascular glomeruluses and undeveloped tubules along with neoplastic cells lined up around vessels were seen in many areas. Occasional cystic forms, mitotic figures. and intensive necroses A ere present in these areas. Stroma was hyperemic and was full of intensive loose and myxomatous vessels. In addition, metaplastic cartilage near capsule was found in the cortex. Immunohistochemically, a strong immunoreactivity was detected with cytokeratin staining only in primitive tubular epithelia and vimentin staining only in blastemal and stromal areas. This is the first report that, a triphasic nephroblastoma (tumour) was diagnosed in an Angora rabbit using histopathological and inimunohistochemical examinations

Unusual metastases of renal cell carcinoma in the thyroid in a dog

This report describes the case of a bilateral RCC (renal cell carcinoma) with unusual thyroid metastases in a 9 year-old male Anatolian Karabash dog with a history of lethargy, anorexia, depression, dyspnoea and snoring. At necropsy, external and internal solitary masses were found in the left and right kidneys respectively and tumoral nodules were detected in the left adrenal gland, in the regional lymphatic nodes, in lungs and also in the totally necrotic left and right thyroid glands. Histologically, the neoplastic tissues showed solid and tubular cellular structures typical of carcinoma. The RCC diagnosis was confirmed by the double vimentin (V9) and cytokeratin (AE1/AE3) immuno-labelling in the primary renal tumours as well as in the secondary tumours. This is the first report showing thyroid metastases from RCC in the dog