3 research outputs found
Identification of nuclear genes affecting 2-Deoxyglucose resistance inSchizosaccharomyces pombe
2-Deoxyglucose (2-DG) is a toxic glucose analog. To identify genes involved in 2-DG toxicity in Schizosaccharomyces pombe, we
screened a wild-type overexpression library for genes which render cells 2-DG resistant. A gene we termed odr1, encoding
an uncharacterized hydrolase, led to strong resistance and altered invertase expression when overexpressed. We speculate
that Odr1 neutralizes the toxic form of 2-DG, similar to the Saccharomyces cerevisiae Dog1 and Dog2 phosphatases which
dephosphorylate 2-DG-6-phosphate synthesized by hexokinase. In a complementary approach, we screened a haploid
deletion library to identify 2-DG-resistant mutants. This screen identified the genes snf5, ypa1, pas1 and pho7. In liquid
medium, deletions of these genes conferred 2-DG resistance preferentially under glucose-repressed conditions. The
deletion mutants expressed invertase activity more constitutively than the control strain, indicating defects in the control
of glucose repression. No S. cerevisiae orthologs of the pho7 gene is known, and no 2-DG resistance has been reported for any
of the deletion mutants of the other genes identified here. Moreover, 2-DG leads to derepressed invertase activity in S.
pombe, while in S. cerevisiae it becomes repressed. Taken together, these findings suggest that mechanisms involved in 2-DG
resistance differ between budding and fission yeasts
Draft genome of Trypanosoma b. brucei EATRO1125 (AnTaR1 serodeme) strain
Trypanosoma brucei spp, unicellular parasites causing human and animal trypanosomiasis, are transmitted between mammals by tsetse flies. Periodic changes in variant surface glycoproteins (VSG) which form the parasite coat in the mammal, allow them to evade the host immune response. Different isolates of T. brucei show heterogeneity in their repertoires of VSG genes and have single nucleotide polymorphisms that can impact on genome editing. T. b. brucei EATRO1125 (AnTaR1 serodeme) is an isolate that is used increasingly often because it is pleomorphic in mammals and fly transmissible, two characteristics that have been lost by the most commonly used laboratory stocks. We present a draft genome of EATRO1125, including contigs for the intermediate and mini-chromosomes that serve as repositories of VSG genes
Trypanosoma brucei EATRO1125 draft genome assembly (Contigs)
Trypanosoma brucei ssp, unicellular parasites causing human and animal trypanosomiasis, are transmitted between mammals by tsetse flies. Periodic changes in variant surface glycoproteins (VSG), which form the parasite coat in the mammal, allow them to evade the host immune response. Different isolates of T. brucei show heterogeneity in their repertoires of VSG genes and have single nucleotide polymorphisms and indels that can impact on genome editing. T. brucei brucei EATRO1125 (AnTaR1 serodeme) is an isolate that is used increasingly often because it is pleomorphic in mammals and fly transmissible, two characteristics that have been lost by the most commonly used laboratory stocks. We present a genome assembly of EATRO1125, including contigs for the intermediate and mini-chromosomes that serve as repositories of VSG genes. In addition, de novo transcriptome assemblies were performed using Illumina sequences from tsetse-derived trypanosomes