Elckerlyc goes mobile - Enabling natural interaction in mobile user interfaces

The fast growth of computational resources and speech technology available on mobile devices makes it possible to entertain users of these devices in having a natural dialogue with service systems. These systems are sometimes perceived as social agents and this can be supported by presenting them on the interface by means of an animated embodied conversational agent. To take the full advantage of the power of embodied conversational agents in service systems it is important to support real-time, online and responsive interaction with the system through the embodied conversational agent. The design of responsive animated conversational agents is a daunting task. Elckerlyc is a model-based platform for the specification and animation of synchronised multi-modal responsive animated agents. This paper presents a new light-weight PictureEngine that allows to run this platform in mobile applications. We describe the integration of the PictureEngine in the user interface of two different coaching applications and discuss the findings from user evaluations. We also conducted a study to evaluate an editing tool for the specification of the agent’s communicative behaviour. Twenty one participants had to specify the behaviour of an embodied conversational agent using the PictureEngine. We may conclude that this new lightweight back-end engine for the Elckerlyc platform makes it easier to build embodied conversational interfaces for mobile devices

A Randomized Trial to Assess Anti-HIV Activity in Female Genital Tract Secretions and Soluble Mucosal Immunity Following Application of 1% Tenofovir Gel

Preclinical and early phase clinical microbicide studies have not consistently predicted the outcome of efficacy trials. To address this gap, candidate biomarkers of microbicide pharmacodynamics and safety were evaluated in a double-blind, placebo-controlled trial of tenofovir gel, the first microbicide to demonstrate significant protection against HIV acquisition.30 women were randomized to apply a single daily dose of tenofovir or placebo gel for 14 consecutive days. Anti-HIV activity was measured in cervicovaginal lavage (CVL) on Days 0, 3, 7, 14 and 21 by luciferase assay as a surrogate marker of pharmacodynamics. Endogenous activity against E. coli and HSV-2 and concentrations of immune mediators were quantified in CVL as candidate biomarkers of safety. Tenofovir levels were measured in CVL and blood.A significant increase in anti-HIV activity was detected in CVL from women who applied tenofovir gel compared to their endogenous anti-HIV activity in genital tract secretions on Day 0 and compared to activity in CVL from women in the placebo group. The activity correlated significantly with CVL concentration of tenofovir (r = 0.6, p<0.001) and fit a sigmoid E(max) pharmacodynamic model. Anti-HIV activity in CVL from women who applied tenofovir persisted when virus was introduced in semen, whereas endogenous anti-HIV activity decreased. Tenofovir did not trigger an inflammatory response or induce sustained loss in endogenous antimicrobial activity or immune mediators.Tenofovir gel had no deleterious impact on soluble mucosal immunity. The increased anti-HIV activity in CVL, which persisted in the presence of semen and correlated with tenofovir concentration, is consistent with the efficacy observed in a recent clinical trial. These results promote quantified CVL anti-HIV activity as a surrogate of tissue pharmacodynamics and as a potential biomarker of adherence to product. This simple, feasible and inexpensive bioassay may promote the development of models more predictive of microbicide efficacy.ClinicalTrials.gov NCT00594373

Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators

Background: Measurement of immune mediators and antimicrobial activity in female genital tract secretions may provide biomarkers predictive of risk for HIV-1 acquisition and surrogate markers of microbicide safety. However, optimal methods for sample collection do not exist. This study compared collection methods. Methods: Secretions were collected from 48 women (24 with bacterial vaginosis [BV]) using vaginal and endocervical Dacron and flocked swabs. Cervicovaginal lavage (CVL) was collected with 10 mL of Normosol-R (n = 20), saline (n = 14), or water (n = 14). The concentration of gluconate in Normosol-R CVL was determined to estimate the dilution factor. Cytokine and antimicrobial mediators were measured by Luminex or ELISA and corrected for protein content. Endogenous anti-HIV-1 and anti-E. coli activity were measured by TZM-bl assay or E. coli growth. Results: Higher concentrations of protein were recovered by CVL, despite a 10-fold dilution of secretions, as compared to swab eluents. After protein correction, endocervical swabs recovered the highest mediator levels regardless of BV status. Endocervical and vaginal flocked swabs recovered significantly higher levels of anti-HIV-1 and anti-E. coli activity than Dacron swabs (P<0.001). BV had a significant effect on CVL mediator recovery. Normosol-R tended to recover higher levels of most mediators among women with BV, whereas saline or water tended to recover higher levels among women without BV. Saline recovered the highest levels of anti-HIV-1 activity regardless of BV status. Conclusions: Endocervical swabs and CVL collected with saline provide the best recovery of most mediators and would be the optimal sampling method(s) for clinical trials. © 2011 Dezzutti et al

Discovery of a Potential Human Serum Biomarker for Chronic Seafood Toxin Exposure Using an SPR Biosensor

Domoic acid (DA)-producing harmful algal blooms (HABs) have been present at unprecedented geographic extent and duration in recent years causing an increase in contamination of seafood by this common environmental neurotoxin. The toxin is responsible for the neurotoxic illness, amnesic shellfish poisoning (ASP), that is characterized by gastro-intestinal distress, seizures, memory loss, and death. Established seafood safety regulatory limits of 20 &#956;g DA/g shellfish have been relatively successful at protecting human seafood consumers from short-term high-level exposures and episodes of acute ASP. Significant concerns, however, remain regarding the potential impact of repetitive low-level or chronic DA exposure for which there are no protections. Here, we report the novel discovery of a DA-specific antibody in the serum of chronically-exposed tribal shellfish harvesters from a region where DA is commonly detected at low levels in razor clams year-round. The toxin was also detected in tribal shellfish consumers&#8217; urine samples confirming systemic DA exposure via consumption of legally-harvested razor clams. The presence of a DA-specific antibody in the serum of human shellfish consumers confirms long-term chronic DA exposure and may be useful as a diagnostic biomarker in a clinical setting. Adverse effects of chronic low-level DA exposure have been previously documented in laboratory animal studies and tribal razor clam consumers, underscoring the potential clinical impact of such a diagnostic biomarker for protecting human health. The discovery of this type of antibody response to chronic DA exposure has broader implications for other environmental neurotoxins of concern

Correction: Performance of Swabs, Lavage, and Diluents to Quantify Biomarkers of Female Genital Tract Soluble Mucosal Mediators

BACKGROUND: Measurement of immune mediators and antimicrobial activity in female genital tract secretions may provide biomarkers predictive of risk for HIV-1 acquisition and surrogate markers of microbicide safety. However, optimal methods for sample collection do not exist. This study compared collection methods. METHODS: Secretions were collected from 48 women (24 with bacterial vaginosis [BV]) using vaginal and endocervical Dacron and flocked swabs. Cervicovaginal lavage (CVL) was collected with 10 mL of Normosol-R (n = 20), saline (n = 14), or water (n = 14). The concentration of gluconate in Normosol-R CVL was determined to estimate the dilution factor. Cytokine and antimicrobial mediators were measured by Luminex or ELISA and corrected for protein content. Endogenous anti-HIV-1 and anti-E. coli activity were measured by TZM-bl assay or E. coli growth. RESULTS: Higher concentrations of protein were recovered by CVL, despite a 10-fold dilution of secretions, as compared to swab eluents. After protein correction, endocervical swabs recovered the highest mediator levels regardless of BV status. Endocervical and vaginal flocked swabs recovered significantly higher levels of anti-HIV-1 and anti-E. coli activity than Dacron swabs (P<0.001). BV had a significant effect on CVL mediator recovery. Normosol-R tended to recover higher levels of most mediators among women with BV, whereas saline or water tended to recover higher levels among women without BV. Saline recovered the highest levels of anti-HIV-1 activity regardless of BV status. CONCLUSIONS: Endocervical swabs and CVL collected with saline provide the best recovery of most mediators and would be the optimal sampling method(s) for clinical trials

Levels of immune mediators and endogenous antimicrobial activity in female genital tract samples by CVL diluents.

a<p>CVL, cervicovaginal lavage.</p>b<p>% inhibition – negative values reflect increased growth of <i>E. coli</i> or enhancement of infection of HIV-1.</p>c<p>median (range).</p>d<p>nt = not tested.</p>e<p>p-value: Global p-value based on the comparison of protein-adjusted, log-transformed immune mediators and endogenous antimicrobial activity by CVL diluents.</p

Levels of antimicrobial activity in female genital tract secretions collected by cervicovaginal lavages (CVL) and swabs.

<p>Female genital tract secretions were collected by Dacron swabs (DS) and flocked swabs (FS) from the vagina and the endocervix (cervix) and by CVL using Normosol-R, saline, or water. Anti-<i>E. coli</i> activity was calculated as the % inhibition of bacterial growth compared to an untreated control. Anti-HIV-1 activity was calculated as the % inhibition of infection as compared to an untreated control. CVL/water was not tested (nt) because water would lyse the pathogens or cells providing non-reportable results. Negative values reflect increased <i>E. coli</i> growth or enhanced HIV-1 infection. Data are presented as box and whisker plots where the median is the horizontal line through the vertical box which represents the 25–75^th percentiles. Values within the 10–90^th percentiles are represented by the error bars. Outliers are shown by filled circles. Significant changes were determined using linear mixed model and discussed in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023136#s3" target="_blank">results</a> section.</p

Protein levels in female genital tract secretions collected by swabs and cervicovaginal lavages (CVL).

<p>Female genital tract secretions were collected by Dacron swabs (DS) and flocked swabs (FS) from the vagina and the endocervix (cervix) and by CVL using Normosol-R, saline, or water. Protein levels were determined using the Bradford assay. Data are presented as box and whisker plots where the median is the horizontal line through the vertical box which represents the 25–75^th percentiles. Values within the 10–90^th percentiles are represented by the error bars. Outliers are shown by filled circles. Data were log-transformed and significant changes were determined using linear mixed model and discussed in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023136#s3" target="_blank">results</a> section.</p

Sample collection algorithm.

<p>Sample collection proceeded from the introitus to the cervix. A Dacron swab was rolled 360° along the vaginal lateral wall. A flocked swab was rolled 360° along the opposite vaginal lateral wall. After vaginal swabs, endocervical swabs were taken with first a Dacron swab and then a flocked swab inserted into the cervical os and turned 360°. Finally, the women were randomized to have a CVL collected with 10 ml of saline, Normosol-R, or tap water. Samples were processed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023136#s2" target="_blank">Methods</a> section.</p