Identification and sequence comparison of a cuticular collagen of Brugia pahangi

The cuticle of filarial nematodes is a specialized extracellular matrix that covers the parasite and protects it from adverse conditions of the environment. As a surface structure it is in direct contact with the host defence mechanisms and therefore plays an important role in the molecular host-parasite relationship. Using polyclonal antisera raised against the insoluble components of the cuticle of the adult filarial parasite Brugia pahangi, we have isolated cDNA clones encoding collagen molecules of the cuticle. The protein domain structure of cDNA clone Bpcol-1 was compared with the known structures of cuticular collagens of the nematodes Brugia malayi, Caenorhabditis elegans, Ascaris suum and Haemonchus contortus, confirming interspecies similarities. Using affinity-purified anti-Bpcol-1 antibodies we identified Bpcol-1 antigenic determinants in different nematode extracts, and determined the localization of such epitopes within the cuticle of B. pahang

Interaction between gravitational waves and plasma waves in the Vlasov description

The nonlinear interaction between electromagnetic, electrostatic and gravitational waves in a Vlasov plasma is reconsidered. By using a orthonormal tetrad description the three-wave coupling coefficients are computed. Comparing with previous results, it is found that the present theory leads to algebraic expression that are much reduced, as compared to those computed using a coordinate frame formalism. Furthermore, here we calculate the back-reaction on the gravitational waves, and a simple energy conservation law is deduced in the limit of a cold plasma.Comment: 9 pages, uses jpp.cl

Determination of Acaricide Resistance in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Field Populations of Argentina, South Africa, and Australia With the Larval Tarsal Test

Infestations with ticks have an important economic impact on the cattle industry worldwide and resistance to acaricides has become a widespread phenomenon. To optimize their treatment strategy, farmers need to know if and against which classes potential acaricide-resistance does occur. Bioassays are used to assess the resistance level and pattern of Rhipicephalus (Boophilus) microplus populations. The objective of the current study was to assess the susceptibility of field populations originating from Argentina (8), South Africa (3), and Australia (2) using the Larval Tarsal Test. Nine acaricidal compounds from five major classes were tested: organosphosphates, synthetic pyrethroids (SP), macrocyclic lactones, phenylpyrazols, and amidines. The resistance ratios at concentrations inducing 50 and 90% mortality were used to detect established and emerging resistance. This study confirmed the newly reported presence of amitraz resistance in populations from Argentina. In addition, resistance to SP appeared to be widespread (88%) in the Argentinean farms, which had been selected based on the observation of lack of treatment efficacy by farmers. In South Africa one of the three populations was found to be resistant to SP and to a phenylpyrazol compound (pyriprol). Furthermore, resistance to organosphosphates and SP was observed in Australia. Finally, the Larval Tarsal Test proved to be a suitable test to evaluate the susceptibility of R. microplus field populations to the most relevant acaricidal classe

Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms

Nucleosome filaments of two stages of the life-cycle of Trypanosoma brucei brucei, namely bloodstream forms and procyclic culture forms, were investigated by electron microscopy. Chromatin of bloodstream forms showed a salt-dependent condensation. The level of condensation was higher than that shown by chromatin from procyclic culture forms, but 30 nm fibres as formed in rat liver chromatin preparations were not found. Analysis of histones provided new evidence for the existence of H1-like proteins, which comigrated in the region of the core histones in SDS-PAGE and in front of the core histones in Triton acid urea gels. Differences were found between the H1-like proteins of the two trypanosome stages as well as between the core histones in their amount, number of bands and banding pattern. It can be concluded that T. b. brucei contains a full set of histones, including H1-like proteins, and that the poor condensation of its chromatin is not due to the absence of H1, but most probably due to histone-DNA interaction being weak. It is obvious that structural and functional differences of the chromatin exist not only between T. b. brucei and higher eukaryotes, but also between various stages of the life-cycle of the parasite. It is therefore not adequate to investigate the chromatin only of the procyclic culture forms as a model for all stages of the life-cycle of T. b. bruce

Cosmic magnetic fields from velocity perturbations in the early Universe

We show, using a covariant and gauge-invariant charged multifluid perturbation scheme, that velocity perturbations of the matter-dominated dust Friedmann-Lemaitre-Robertson-Walker (FLRW) model can lead to the generation of cosmic magnetic fields. Moreover, using cosmic microwave background (CMB) constraints, it is argued that these fields can reach strengths of between 10^{-28} and 10^{-29} G at the time the dynamo mechanism sets in, making them plausible seed field candidates.Comment: 11 pages, 1 figure, IOP style, minor changes and typos correcte

Histomorphological analysis of the urogenital diaphragm in elderly women: a cadaver study

The objective of this study was to describe the histomorphological structure of the urogenital diaphragm in elderly women using a modern morphometric procedure. Biopsies were taken from the posterior margin of the urogenital diaphragm of 22 female cadavers (mean age, 87years) using a 60-mm punch. Hematoxylin/eosin and Goldner sections were analyzed with the Cavalieri estimator. The mean thickness of the urogenital diaphragm was 5.5mm. The main component was connective tissue. All biopsies contained smooth muscle. Eighteen biopsies contained more smooth muscle than striated muscle. In six of 22 biopsies, no striated muscle was found. The ratio of striated to smooth muscle to connective tissue was 1:2.3:13.3. Muscle fibers were dispersed in all parts of the urogenital diaphragm. The urogenital diaphragm of elderly women mainly consists of connective tissue. Smooth muscle was also found but to a lesser extent. The frequently used English term "perineal membrane” for the urogenital diaphragm is justified and well describes our findings in elderly wome

DBI with Primordial Magnetic Field in the Sky

In this paper, we study the generation of a large scale magnetic field with amplitude of order $\mu$G in an inflationary model which has been introduced in hep-th/0310221. This inflationary model based on existence of a speed limit for inflaton field. Generating a mass for inflaton at scale above the $\phi_{IR}$, breaks the conformal triviality of the Maxwell equation and causes to originate a magnetic field during the inflation. The amplitude strongly depends on the details of reheating stage and also depends on the e-foldings parameter N. We find the amplitude of the primordial magnetic field at decoupling time in this inflationary background using late time behavior of the theory.Comment: 12 pages, no figure, typos correcte

Charged multifluids in general relativity

The exact 1+3 covariant dynamical fluid equations for a multi-component plasma, together with Maxwell's equations are presented in such a way as to make them suitable for a gauge-invariant analysis of linear density and velocity perturbations of the Friedmann-Robertson-Walker model. In the case where the matter is described by a two component plasma where thermal effects are neglected, a mode representing high-frequency plasma oscillations is found in addition to the standard growing and decaying gravitational instability picture. Further applications of these equations are also discussed.Comment: 14 pages (example added), to appear in Class. Quantum Gra