3 research outputs found
Differential Localization of G Protein βγ Subunits
G protein βγ subunits
play essential roles in regulating
cellular signaling cascades, yet little is known about their distribution
in tissues or their subcellular localization. While previous studies
have suggested specific isoforms may exhibit a wide range of distributions
throughout the central nervous system, a thorough investigation of
the expression patterns of both Gβ and Gγ isoforms within
subcellular fractions has not been conducted. To address this, we
applied a targeted proteomics approach known as multiple-reaction
monitoring to analyze localization patterns of Gβ and Gγ
isoforms in pre- and postsynaptic fractions isolated from cortex,
cerebellum, hippocampus, and striatum. Particular Gβ and Gγ
subunits were found to exhibit distinct regional and subcellular localization
patterns throughout the brain. Significant differences in subcellular
localization between pre- and postsynaptic fractions were observed
within the striatum for most Gβ and Gγ isoforms, while
others exhibited completely unique expression patterns in all four
brain regions examined. Such differences are a prerequisite for understanding
roles of individual subunits in regulating specific signaling pathways
throughout the central nervous system