Study protocol for a randomised controlled trial evaluating the effect of prenatal omega-3 LCPUFA supplementation to reduce the incidence of preterm birth: The ORIP trial

Introduction: Preterm birth accounts for more than 85% of all perinatal complications and deaths. Seventy-five per cent of early preterm births (EPTBs) occur spontaneously and without identifiable risk factors. The need for a broadly applicable, effective strategy for primary prevention is paramount. Secondary outcomes from the docosahexaenoic acid (DHA) to Optimise Mother Infant Outcome trial showed that maternal supplementation until delivery with omega-3 (ω-3) long chain polyunsaturated fatty acid (LCPUFA), predominantly as DHA, resulted in a 50% reduction in the incidence of EPTB and an increase in the incidence of post-term induction or post-term prelabour caesarean section due to extended gestation. We aim to determine the effectiveness of supplementing the maternal diet with ω-3 LCPUFA until 34 weeks’ gestation on the incidence of EPTB. Methods and analysis: This is a multicentre, parallel group, randomised, blinded and controlled trial. Women less than 20 weeks’ gestation with a singleton or multiple pregnancy and able to give informed consent are eligible to participate. Women will be randomised to receive high DHA fish oil capsules or control capsules without DHA. Capsules will be taken from enrolment until 34 weeks’ gestation. The primary outcome is the incidence of EPTB, defined as delivery before 34 completed weeks’ gestation. Key secondary outcomes include length of gestation, incidence of post-term induction or prelabour caesarean section and spontaneous EPTB. The target sample size is 5540 women (2770 per group), which will provide 85% power to detect an absolute reduction in the incidence of preterm birth of 1.16% (from 2.45% to 1.29%) between the DHA and control group (two sided α=0.05). The primary analysis will be based on the intention-to-treat principle. Trial registration number: Australia and New Zealand Clinical Trial Registry Number: 2613001142729; Pre-results

Hypervelocity Impact Testing of Space Station Freedom Solar Cells

Solar array coupons designed for the Space Station Freedom electrical power system were subjected to hypervelocity impacts using the HYPER facility in the Space Power Institute at Auburn University and the Meteoroid/Orbital Debris Simulation Facility in the Materials and Processes Laboratory at the NASA Marshall Space Flight Center. At Auburn, the solar cells and array blanket materials received several hundred impacts from particles in the micron to 100 micron range with velocities typically ranging from 4.5 to 10.5 km/s. This fluence of particles greatly exceeds what the actual components will experience in low earth orbit. These impacts damaged less than one percent of total area of the solar cells and most of the damage was limited to the cover glass. There was no measurable loss of electrical performance. Impacts on the array blanket materials produced even less damage and the blanket materials proved to be an effective shield for the back surface of the solar cells. Using the light gas gun at MSFC, one cell of a four cell coupon was impacted by a 1/4 inch spherical aluminum projectile with a velocity of about 7 km/s. The impact created a neat hole about 3/8 inch in diameter. The cell and coupon were still functional after impact

Hypervelocity impact induced arcing and Kapton pyrolization in a plasma environment

Tests were performed on the Space Station Freedom (SSF) solar array flat conductor circuit (FCC) to determine if hypervelocity impacts could induce pyrolization of Kapton and/or cross-conductor arcing. A sample piece of FCC was placed in a plasma environment and biased to +200 V relative to the plasma potential. The FCC was then impacted with particles in the 100 micron size range with hypervelocities of about 7 km/s. These tests were unable to induce Kapton pyrolization, cross-conductor arcing, or any other plasma interaction

The Hawaii Infrared Parallax Program. V. New T-Dwarf Members and Candidate Members of Nearby Young Moving Groups

We present a search for new planetary-mass members of nearby young moving groups (YMGs) using astrometry for 694 T and Y dwarfs, including 447 objects with parallaxes, mostly produced by recent large parallax programs from UKIRT and Spitzer. Using the BANYAN $\Sigma$ and LACEwING algorithms, we identify 30 new candidate YMG members, with spectral types of T0$-$T9 and distances of $10-43$ pc. Some candidates have unusually red colors and/or faint absolute magnitudes compared to field dwarfs with similar spectral types, providing supporting evidence for their youth, including 4 early-T dwarfs. We establish one of these, the variable T1.5 dwarf 2MASS J21392676$+$0220226, as a new planetary-mass member ($14.6^{+3.2}_{-1.6}$ M$_{\rm Jup}$) of the Carina-Near group ($200\pm50$ Myr) based on its full six-dimensional kinematics, including a new parallax measurement from CFHT. The high-amplitude variability of this object is suggestive of a young age, given the coexistence of variability and youth seen in previously known YMG T dwarfs. Our four latest-type (T8$-$T9) YMG candidates, WISE J031624.35$+$430709.1, ULAS J130217.21$+$130851.2, WISEPC J225540.74$-$311841.8, and WISE J233226.49$-$432510.6, if confirmed, will be the first free-floating planets ($\approx2-6$ M$_{\rm Jup}$) whose ages and luminosities are compatible with both hot-start and cold-start evolutionary models, and thus overlap the properties of the directly-imaged planet 51 Eri b. Several of our early/mid-T candidates have peculiar near-infrared spectra, indicative of heterogenous photospheres or unresolved binarity. Radial velocity measurements needed for final membership assessment for most of our candidates await upcoming 20$-$30 meter class telescopes. In addition, we compile all 15 known T7$-$Y1 benchmarks and derive a homogeneous set of their effective temperatures, surface gravities, radii, and masses.Comment: ApJ, in press. 27 pages including 6 figures and 5 table

Synthesis, characterization and modelling of zinc and silicate co-substituted hydroxyapatite.

Experimental chemistry and atomic modelling studies were performed here to investigate a novel ionic co-substitution in hydroxyapatite (HA). Zinc, silicate co-substituted HA (ZnSiHA) remained phase pure after heating to 1100 °C with Zn and Si amounts of 0.6 wt% and 1.2 wt%, respectively. Unique lattice expansions in ZnSiHA, silicate Fourier transform infrared peaks and changes to the hydroxyl IR stretching region suggested Zn and silicate co-substitution in ZnSiHA. Zn and silicate insertion into HA was modelled using density functional theory (DFT). Different scenarios were considered where Zn substituted for different calcium sites or at a 2b site along the c-axis, which was suspected in singly substituted ZnHA. The most energetically favourable site in ZnSiHA was Zn positioned at a previously unreported interstitial site just off the c-axis near a silicate tetrahedron sitting on a phosphate site. A combination of experimental chemistry and DFT modelling provided insight into these complex co-substituted calcium phosphates that could find biomedical application as a synthetic bone mineral substitute.This work was supported by a NSFGRFP grant (DGE-1042796) (RJF) and a Cambridge International Scholarship (RJF). The modelling work was performed using the Darwin Supercomputer of the University of Cambridge High Performance Computing Service (http://www.hpc.cam.ac.uk/), provided by Dell Inc. using Strategic Research Infrastructure Funding from the Higher Education Funding Council for England and funding from the Science and Technology Facilities Council. HC would like to thank the UK Medical Research Council (Grant number U105960399) for their support.This is the final version of the article. It first appeared from Royal Society Publishing via http://dx.doi.org/10.1098/rsif.2015.019

Atomistic mechanism of transmembrane helix association

Transmembrane helix association is a fundamental step in the folding of helical membrane proteins. The prototypical example of this association is formation of the glycophorin dimer. While its structure and stability have been well-characterized experimentally, the detailed assembly mechanism is harder to obtain. Here, we use all-atom simulations within phospholipid membrane to study glycophorin association. We find that initial association results in the formation of a non-native intermediate, separated by a significant free energy barrier from the dimer with a native binding interface. We have used transition-path sampling to determine the association mechanism. We find that the mechanism of the initial bimolecular association to form the intermediate state can be mediated by many possible contacts, but seems to be particularly favoured by formation of non-native contacts between the C-termini of the two helices. On the other hand, the contacts which are key to determining progression from the intermediate to the native state are those which define the native binding interface, reminiscent of the role played by native contacts in determining folding of globular proteins. As a check on the simulations, we have computed association and dissociation rates from the transition-path sampling. We obtain results in reasonable accord with available experimental data, after correcting for differences in native state stability. Our results yield an atomistic description of the mechanism for a simple prototype of helical membrane protein folding