48 research outputs found
Research of <i>PNPLA3</i> I148M Gene Polymorphism in Patients with Non-Alcoholic Fatty Liver Disease, with Liver Cirrhosis and with Hepatocellular Carcinoma
Aim: to determine the frequency of PNPLA3 rs738409 C>G gene polymorphism, leading to p.I148M substitution, in patients with non-alcoholic fatty liver disease (NAFLD), and to reveal the association between polymorphism and probable NAFLD outcomes: liver cirrhosis (LC) and hepatocellular carcinoma (HCC).Materials and methods. The study was conducted according to the βcase-controlβ design, three main groups were formed: a group with NAFLD (n = 46), a group with LC (n = 61), a group with HCC (n = 50), as well as a control group (n = 70), for all groups we performed genotyping of the rs738409 polymorphism of the PNPLA3 gene. The relationship between the occurrence of different genotype variants and the diagnosis of patients was evaluated, the odds ratio (OR) of progression of NAFLD and the reliability of intergroup differences were determined.Results. NAFLD patients with PNPLA3 I148M polymorphism have a significantly higher chance of developing LC and HCC. The odds ratio for the GG genotype was 7.94 (95 % Cl: 2.19β28.84; p = 0.030) for LC and 6.51 (95 % Cl: 1.15β4.08; p = 0.039) β for HCC with concomitant LC. The presence of the minor G allele also increases the likelhood of transition from NAFLD to LC (OR = 2.38; 95 % Cl: 1.41β4.02; p = 0.010) and HCC in the presence of cirrhosis (OR = 2.17; 95 % Cl: 1.15β4.08; p = 0.039). Differences in the frequency of PNPLA3 polymorphism between the NAFLD and HCC groups were not significant. Additional risk factors for HCC associated with NAFLD are overweight (OR = 5.14; 95 % Cl: 1.94β13.67; p < 0.001), arterial hypertension (OR = 8.49; 95 % Cl: 3.05β23,62; p < 0.001) and diabetes mellitus (OR = 8.57; 95 % Cl: 1.03β71.48; p = 0.032).Conclusion. The frequency of single nucleotide polymorphism PNPLA3 significantly differs in patients with NAFLD, cirrhosis and HCC compared with the control group of healthy volunteers. The PNPLA3 I148M polymorphism increases the incidence of NAFLD progression to cirrhosis and HCC, but only with concomitant cirrhosis
Risk Factors for Obesity Development in Different Periods of Childhood
Obesity is an important health problem in many countries. Obesity among the child population is growing steadily, including the Russian Federation. Development of this disease often occurs in childhood and sometimes the origin of obesity goes back to prenatal period. There are a number of endogenous and exogenous factors than play an important role in development of obesity. These are heredity, socioeconomic status of the family, factors which are revealed during pregnancy and child delivery β weight gain, administration of antibacterial drugs and hyperglycemia in mother during her pregnancy, mode of delivery, feeding type and time of complementary food introduction, excessive consumption of calories with food, improper daily routine and lack of sleep, skipping meals, use of gadgets and associated physical inactivity and excessive food intake, marketing of high-calorie foods and others. Prevailing risk factors can be identified for each age period. Study and early identification of risk factors taking into account age of a child is necessary to take timely prevention measures and inform parents and their children about possible reasons and consequences of obesity
Characteristics of blood pressure level in children with different body weight
BACKGROUND: Essential arterial hypertension (AH) develops more often in children with accompanying risk factors β obesity, overweight, positive heredity and genetic predisposition.AIM: Study of peculiarities of arterial hypertension clinical course in adolescents with normal body weight, overweight and obesity.MATERIALS AND METHODS: The study was conducted on children with arterial hypertension who received treatment in two hospitals in Voronezh in 2016β2020. A retrospective analysis of the childrenβs case histories was carried out taking into account the anamnesis, clinical laboratory and instrumental examination data and the pharmacotherapy. Some children underwent polymerase chain reaction genetic testing to determine pathological alleles of genes regulating blood pressure (BP).RESULTS: 96 patients aged 9 to 17 took part in the study. The group with normal body weight included 38 children (39.6%), median age 16.4 (aged 10.7; 17.9), with overweight β 33 people (34.4%), median age 15.2 (aged 12.0; 17.9), with obesity β 25 children (26.0%), median age 14.5 (aged 9.2; 17.9). Obese children developed arterial hypertension at earlier age (p = 0.023). According to blood pressure daily monitoring (BPDM), pathological values of systolic blood pressure (SBP) during the day (above the 95th percentile) among children with normal body weight were observed in 17 patients (44.7%), with excess body weight β in 14 people (42.4%), with obesity β in 16 people (64%), p = 0.031. Accurate difference values between the groups were obtained in terms of time index (TI) of SBP at night (p = 0.006). Time index of diastolic BP during theΒ day > 50% was observed only in the obese children group β 4 people (16%) (p = 0.042). Pathological alleles of the angiotensinogen gene (AGT: 704 T>C), aldosterone synthase gene (CYP11B2: -344 C>T) and endothelial nitrogen synthase typeΒ 3 (NOS3: -786 T> C) were identified most frequently during genetic testing in some patients.CONCLUSION: Children with obesity developed earlier arterial hypertension compared to the same-age children with normal body weight and more often had unfavorable type of arterial hypertension according to BPDM. These results can be used to choose individual therapy and to develop special attention as regards certain target organs damage
Diet peculiarities of organized preschoolers living in families with different material well-being status
The family well-being can affect the approaches to the organization of the childβs nutrition and its future health. The aim of this study was to evaluate the style and quality of diet among children ages 3 to 7 years in families with different financial status according to the parentsβ self-assessment. The cross-section study included 190 organized preschool children that were divided into the 2 groups. The first group included 83 children with a sufficient family income; the 2nd group included 107 children with an insufficient level of family income. We revealed that the children in families with higher social and financial status consumed the fruits 1.5 times more often and had the defects of diet 2 times less than children with lower economic status. At the same time all children not enough eat fish, meat, vegetables, cereals, fruits.Π£ΡΠΎΠ²Π΅Π½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π±Π»Π°Π³ΠΎΠΏΠΎΠ»ΡΡΠΈΡ ΡΠ΅ΠΌΡΠΈ ΠΌΠΎΠΆΠ΅Ρ ΠΎΠΊΠ°Π·Π°ΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ ΠΏΠΈΡΠ°Π½ΠΈΡ ΡΠ΅Π±Π΅Π½ΠΊΠ° Π² Π΄ΠΎΠΌΠ°ΡΠ½ΠΈΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΈ Π² Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅ΠΌ ΠΎΡΡΠ°Π·ΠΈΡΡΡΡ Π½Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠ³ΠΎ ΠΏΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ΅Π±Π΅Π½ΠΊΠ°. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΎΡΠ΅Π½ΠΈΡΡ ΡΠ΅ΠΆΠΈΠΌ ΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΡΠ°ΡΠΈΠΎΠ½Π° ΠΏΠΈΡΠ°Π½ΠΈΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 3 Π΄ΠΎ 7 Π»Π΅Ρ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΡΠ΅ΠΌΠ΅ΠΉ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΡΠΎΡΠΈΠ°Π»ΡΠ½ΠΎ-ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΡΠ°ΡΡΡΠΎΠΌ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΊΡΠΎΡΡ-ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅, Π² ΠΊΠΎΡΠΎΡΠΎΠ΅ Π±ΡΠ»ΠΎ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 190 ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π΄ΠΎΡΠΊΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°, 1-Ρ Π³ΡΡΠΏΠΏΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 83 ΡΠ΅Π±Π΅Π½ΠΊΠ° Ρ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΠΌΡΠΈ, 2-Ρ Π³ΡΡΠΏΠΏΡ β 107 Π΄Π΅ΡΠ΅ΠΉ Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠ΅ΠΌΠ΅ΠΉΠ½ΠΎΠ³ΠΎ Π΄ΠΎΡ
ΠΎΠ΄Π°. ΠΡΠ»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² Π³ΡΡΠΏΠΏΠ΅ Π΄Π΅ΡΠ΅ΠΉ ΠΈΠ· ΡΠ΅ΠΌΠ΅ΠΉ Ρ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ Π΄ΠΎΠ»Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π² ΡΠ°ΡΠΈΠΎΠ½Π΅ ΠΏΠΈΡΠ°Π½ΠΈΡ ΡΡΡΠΊΡΠΎΠ² Π±ΡΠ»Π° Π² 1,5 ΡΠ°Π·Π° Π²ΡΡΠ΅ (Ρ = 0,02), Π° ΡΠΈΡΠ»ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΠ΅ΠΆΠΈΠΌΠ° ΠΏΠΈΡΠ°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π² 2 ΡΠ°Π·Π° ΠΌΠ΅Π½ΡΡΠ΅ (Ρ = 0,0003), ΡΠ΅ΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠ΅ΠΌΠ΅ΠΉΠ½ΠΎΠ³ΠΎ Π΄ΠΎΡ
ΠΎΠ΄Π°. ΠΡΠΈ ΡΡΠΎΠΌ Π²ΡΠ΅ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΠ΅ Π΄Π΅ΡΠΈ Π΄ΠΎΡΠΊΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΠΏΠΎΡΡΠ΅Π±Π»ΡΠ»ΠΈ Π² ΠΏΠΈΡΡ ΡΡΠ±Ρ, ΠΌΡΡΠΎ, ΠΎΠ²ΠΎΡΠΈ, ΠΊΡΡΠΏΡΠ½ΡΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΡ, ΡΡΡΠΊΡΡ
Major and minor lymphocytes subpopulations in peripheral blood and cerebrospinal fluid of children with meningitis
Introduction. The analysis of current publications indicates at our insufficient understanding of subpopulation composition of lymphocytes in peripheral blood and cerebrospinal fluid (CSF) during pediatric neuroinfectious diseases. It has been found that the main lymphocyte populations are divided into many small (minor) subpopulations.The purpose of this research was to assess percentage of major and minor blood and CSF lymphocyte subsets in children with aseptic viral meningitis (AM) or bacterial purulent meningitis (BM).Materials and methods. Phenotyping of blood and CSF lymphocytes of children aged from 4 months to 17 years diagnosed with AM (n = 86) and BM (n = 39) was carried out by using flow cytometry. As a comparison group, we analyzed peripheral blood and CSF samples collected from children with acute respiratory viral infections (ARVIs) associated with syndrome of meningism (n = 27). There was evaluated percentage of the major cell subpopulations (CD3+ T-lymphocytes, T-helpers β CD3+CD4+ Th, cytotoxic T-lymphocytes β CD3+CD8+ CTL, natural killer cells β CD3-CD16+CD56+ NK, B-cells β CD3-CD19+), as well as minor lymphocyte subsets (double positive (DP) (CD3+CD4+CD8+), double negative (DN) (CD3+CD4-CD8-) T-cells, NKT (CD3+CD16+CD56+), CD3-CD8+ NK, CD3+CD8dim and CD3+CD8 8bright).Results. It was found that the acute period of BM and AM vs. the comparison group (ARVI) was characterized by significant differences in the blood and CSF composition of major and minor lymphocyte subsets. In particular, blood T-cells, Th, CTL, NK, NKT, DN, CD3-CD8+ NK, CD3+CD8bright and CD3+CD8dim dominated in parallel with significantly lowered B-cell frequency in AM vs. BM. In the CSF of children with AM, T-cells and Th prevailed, whereas count of B-cells and CD3-CD8+ NK was lower compared to those in BM. In addition, further differences were revealed in CSF and blood cell subset composition depending on nosological entity, while maintaining differences in some major and minor lymphocyte subpopulations lacked in the comparison group. Calculating the CSF/blood ratio for the major and minor lymphocyte subsets uncovered the prevalence for the majority of cell subpopulations (the coefficients ranged from 1.2 to 16.4) in the CSF of the comparison group (ARVI), except B-cells, NK and CD3-CD8+ NK (coefficients ranged from 0.07 to 0.31). AM and BM were featured with various changes in the CSF/blood ratio found for most of the studied subpopulations in the acute period as well as the recovery phase highlighted with characteristic traits for each nosological form.Conclusion. The data obtained indicate about finding specific features in the activation of systemic and intrathecal immune response during viral and bacterial meningitis in children, which may be used as an additional differential diagnostic criterion
ΠΠ‘ΠΠΠΠΠΠΠ‘Π’Π Π’ΠΠ§ΠΠΠΠ― Π ΠΠ‘Π₯ΠΠΠ« ΠΠΠΠΠΠ’ΠΠΠ¬ΠΠ«Π₯ ΠΠΠΠΠ’ΠΠ’ΠΠ Π ΠΠΠΠΠ§ΠΠΠ ΠΠ’ΠΠΠΠΠΠΠ
We have performed primary examination ofΒ 50 children with neonatal hepatitis. Prevalence of herpesΒ infection as the etiologic agent (40,0%) has been found, asΒ well as parenteral hepatitis, both as an isolated (26,0%) andΒ mixed infections. We conducted a comparative analysis ofΒ clinical and laboratory data at initial examination and determinedΒ the outcomes of neonatal hepatitis to 12 monthsΒ of life depending on the etiology. Congenital CMV- etiologyΒ hepatitis characterized by more cytolysis, mainly due to aspartateΒ aminotransferase and cholestasis, which is 33,3%Β of cases combined by acholia and urobiliya. In 50,0% of patientsΒ with CMV-hepatitis was detected fibrosis with a meanΒ value of liver elastography 9,9 kPa, which is ΠΠΠ degrees ofΒ fibrosis METAVIR scale, whereas in children with primaryΒ chronic hepatitis C and concomitant HCV + DNA-virus infectionΒ fibrosis was not registered. Despite on the large numberΒ of modern non-invasive techniques for determining theΒ degree of hepatic fibrosis, the use of its in children duringΒ the first months of life is limited and does not allow to predictΒ disease outcome.ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
Β Π΄Π°Π½Π½ΡΡ
50 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π½Π΅ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΡΠΌ Π³Π΅ΠΏΠ°ΡΠΈΡΠΎΠΌ Π²Β Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ Ρ ΡΠ΅Π»ΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠ΅ΡΠ΅Π½ΠΈΡΒ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΈ Π΅Π΅ ΠΈΡΡ
ΠΎΠ΄Π° ΠΊ 12 ΠΌΠ΅ΡΡΡΠ°ΠΌ ΠΆΠΈΠ·Π½ΠΈ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈΒ ΠΎΡ ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΡ
ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π°Π½Π°ΠΌΠ½Π΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
Β Π΄Π°Π½Π½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. ΠΡΡΠ²Π»Π΅Π½ΠΎ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠ΅ Π³Π΅ΡΠΏΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π³Π΅Π½ΡΠ°Β (40,0%), Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΠ°ΡΠ΅Π½ΡΠ΅ΡΠ°Π»ΡΠ½ΡΡ
Π³Π΅ΠΏΠ°ΡΠΈΡΠΎΠ² ΠΊΠ°ΠΊ Π² Π²ΠΈΠ΄Π΅Β ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ HCV-ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ (26,0%), ΡΠ°ΠΊ ΠΈ ΡΠΌΠ΅ΡΠ°Π½Π½ΠΎΠΉ HCV+ΠΠΠ-ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π²ΡΠΎΠΆΠ΄Π΅Π½Π½ΡΠ΅ Π³Π΅ΠΏΠ°ΡΠΈΡΡ CMV-ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΡΠΆΠ΅Π»ΠΎ Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌ ΡΠΈΡΠΎΠ»ΠΈΠ·ΠΎΠΌ (ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π·Π° ΡΡΠ΅ΡΒ Π°ΡΠΏΠ°ΡΡΠ°ΡΠ°ΠΌΠΈΠ½ΠΎΡΡΠ°Π½ΡΡΠ΅ΡΠ°Π·Ρ) ΠΈ Ρ
ΠΎΠ»Π΅ΡΡΠ°Π·ΠΎΠΌ, ΠΊΠΎΡΠΎΡΡΠΉ Π²Β 33,3% ΡΠ»ΡΡΠ°Π΅Π² ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ Π°Ρ
ΠΎΠ»ΠΈΠ΅ΠΉ ΠΈ ΡΡΠΎΠ±ΠΈΠ»ΠΈΠ΅ΠΉ. Π£Β 50,0% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ CMV-Π³Π΅ΠΏΠ°ΡΠΈΡΠΎΠΌ Π²ΡΡΠ²Π»Π΅Π½ ΡΠΈΠ±ΡΠΎΠ· ΡΠΎΒ ΡΡΠ΅Π΄Π½ΠΈΠΌ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ»Π°ΡΡΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ 9,9 ΠΊΠΠ° ΠΏΠΎ ΡΠΊΠ°Π»Π΅ METAVIR, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎ-Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌΒ Π³Π΅ΠΏΠ°ΡΠΈΡΠΎΠΌ Π‘ ΠΈ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠΉ HCV+ΠΠΠ-Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ ΡΠΈΠ±ΡΠΎΠ· Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ Π½Π΅ Π±ΡΠ». ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° Π½Π°Π»ΠΈΡΠΈΠ΅ Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
Π½Π΅ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΡ
Β ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊ ΠΏΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΠΈΠ±ΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ, ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΡ
Ρ Π΄Π΅ΡΠ΅ΠΉ ΠΏΠ΅ΡΠ²ΡΡ
ΠΌΠ΅ΡΡΡΠ΅Π² ΠΆΠΈΠ·Π½ΠΈ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΎ ΠΈ Π½Π΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΠΈΡΡ
ΠΎΠ΄ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ
Π‘ΠΈΠ½Π΄ΡΠΎΠΌ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ
The aim of the study was to obtain new biochemical data on the pathogenesis of respiratory syncytial viral infection (RSVI) in children.Object and methods: 60 children aged 1 month to 5 years, treated in the clinic of Pediatric Research and Clinical Center for Infectious Diseases, from which in 50 patients RNA RSV was isolated in smears from the oropharynx. The comparison group consisted of 10 children who failed to verify RSVI by laboratory methods. All children at admission and before discharge from the hospital (after-7-9 days) underwent a clinical blood test a Sysmex XP-300 hematology analyzer (Japan). Alpha-1-antitrypsin and alpha-2-macroglobulin were determined in blood serum by quantitative immunoturbidimetry on a biochemical analyzer CLIMA-15 (Spain) using Sentinel test systems (Italy). Determination of the amount of total protein, albumin and C-reactive protein in serum was carried out on an automatic analyzer Taurus (Instrumentation Laboratory, Italy) using reagents of the company Β«Vector-bestΒ» (Russia). The study of protein fractions in blood serum was carried out by capillary electrophoresis on the device Minicap company Sebia (France) with the help of test systems Β«Minicap Protein(e) 6Β» of the same manufacturer. The levels of cytokines (IL-6, IL-10) in serum were determined by ELISA on ELISA analyzer Β«INFINITIΒ» (TECAN, Austria) using reagents firm Β«Vector-bestΒ» (Russia).Results: RSVI occurs with lesions of the lower respiratory tract in 42% of cases, with the development of complications in 44% of sick children. The study revealed a prolonged increase in serum alpha-2 fraction of globulins, immunoregulatory cytokines with pro-inflammatory (IL-6) and anti-inflammatory (IL-10) action and, which may indicate the presence of subacute inflammatory process associated with the persistence of RS-virus. Lower levels of gamma-globulin fraction, including the main specific and nonspecific immunoglobulins, in children with PCR-proven RSVI, both in the acute period and in the period of convalescence, probably can cause repeated RSV-diseases, as well as an increase in the risk of atopic diseases.Conclusion. The long-term increase in the level of subacute inflammation markers, established in the course of the study, even against the relieve of clinical picture of the disease, makes the question of developing an etiopathogenetic treatment of respiratory syncytial viral infection with the use of drugs with antiviral and anti-inflammatory action relevant.Π¦Π΅Π»Ρ: ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π½ΠΎΠ²ΡΡ
ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΎ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: 60 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 ΠΌΠ΅ΡΡΡΠ° Π΄ΠΎ 5 Π»Π΅Ρ, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π² ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ ΠΠ΅ΡΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΡΠ½ΠΎ-ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ
Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ, ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
Ρ 50 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ Π² ΠΌΠ°Π·ΠΊΠ°Ρ
ΠΈΠ· ΡΠΎΡΠΎΠ³Π»ΠΎΡΠΊΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½Π° Π ΠΠ Π Π‘Π. ΠΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 10 Π΄Π΅ΡΠ΅ΠΉ, Ρ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½Π°Ρ Π²ΠΈΡΡΡΠ½Π°Ρ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π½Π΅ Π²Π΅ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π° Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΡΠ΅ΠΌ Π΄Π΅ΡΡΠΌ ΠΏΡΠΈ ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΠΈ ΠΈ ΠΏΠ΅ΡΠ΅Π΄ Π²ΡΠΏΠΈΡΠΊΠΎΠΉ ΠΈΠ· ΡΡΠ°ΡΠΈΠΎΠ½Π°ΡΠ° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΡΠΎΠ²ΠΈ Π½Π° Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Sysmex XP-300 (Π―ΠΏΠΎΠ½ΠΈΡ). Π ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΡΡΡΠ±ΠΈΠ΄ΠΈΠΌΠ΅ΡΡΠΈΠΈ Π½Π° Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅Β CLIMA-15 (ΠΡΠΏΠ°Π½ΠΈΡ) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌ ΡΠΈΡΠΌΡ Sentinel (ΠΡΠ°Π»ΠΈΡ) ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π°Π»ΡΡΠ°-1Π°Π½ΡΠΈΡΡΠΈΠΏΡΠΈΠ½ ΠΈ Π°Π»ΡΡΠ°-2-ΠΌΠ°ΠΊΡΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΎΠ±ΡΠ΅Π³ΠΎ Π±Π΅Π»ΠΊΠ°, Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π° ΠΈ Π‘-ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Taurus (Instrumentation Laboratory, ΠΡΠ°Π»ΠΈΡ) Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΡΠΈΡΠΌΡ Β«ΠΠ΅ΠΊΡΠΎΡ-ΠΠ΅ΡΡΒ» (Π ΠΎΡΡΠΈΡ). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΡΡΠ°ΠΊΡΠΈΠΉ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° Π½Π° ΠΏΡΠΈΠ±ΠΎΡΠ΅ Minicap ΡΠΈΡΠΌΡ Sebia (Π€ΡΠ°Π½ΡΠΈΡ) Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌ Β«Minicap Protein(Π΅) 6Β» ΡΠΎΠΉ ΠΆΠ΅ ΡΠΈΡΠΌΡ-ΠΈΠ·Π³ΠΎΡΠΎΠ²ΠΈΡΠ΅Π»Ρ. Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (ΠΠ-6, ΠΠ-10) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π½Π° ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Β«INFINITIΒ» (TECAN, ΠΠ²ΡΡΡΠΈΡ) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΡΠΈΡΠΌΡ Β«ΠΠ΅ΠΊΡΠΎΡ-ΠΠ΅ΡΡΒ» (Π ΠΎΡΡΠΈΡ). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½Π°Ρ Π²ΠΈΡΡΡΠ½Π°Ρ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ ΠΏΡΠΎΡΠ΅ΠΊΠ°Π΅Ρ Ρ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ Π½ΠΈΠΆΠ½ΠΈΡ
Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΡΠ΅ΠΉ Π² 42% ΡΠ»ΡΡΠ°Π΅Π², Ρ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ β Ρ 44% Π±ΠΎΠ»ΡΠ½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΏΡΠΎΠ»ΠΎΠ½Π³ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π°Π»ΡΡΠ°-2 ΡΡΠ°ΠΊΡΠΈΠΈ Π³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ², ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ², ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ (ΠΠ-6) ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ (ΠΠ-10) Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ, ΡΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΎ Π½Π°Π»ΠΈΡΠΈΠΈΒ ΠΏΠΎΠ΄ΠΎΡΡΡΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Ρ ΠΏΠ΅ΡΡΠΈΡΡΠ΅Π½ΡΠΈΠ΅ΠΉ Π Π‘-Π²ΠΈΡΡΡΠ°. ΠΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ Π³Π°ΠΌΠΌΠ°-Π³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΡΠ°ΠΊΡΠΈΠΈ, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ΅ΠΉ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Ρ, Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π΄ΠΎΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ, ΠΊΠ°ΠΊ Π² ΠΎΡΡΡΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅, ΡΠ°ΠΊ ΠΈ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ ΡΠ΅ΠΊΠΎΠ½Π²Π°Π»Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°ΡΡ ΠΏΠΎΠ²ΡΠΎΡΠ½ΡΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ Π°ΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΠΎΠ΅ Π² Ρ
ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΏΠΎΠ΄ΠΎΡΡΡΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π΄Π°ΠΆΠ΅ Π½Π° ΡΠΎΠ½Π΅ ΠΊΡΠΏΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½Ρ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π΄Π΅Π»Π°Π΅Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌ Π²ΠΎΠΏΡΠΎΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ ΡΡΠΈΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Ρ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΡΠΌ ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ.