Silk nanoparticles for delivery of human BMP-2 in bone regenerative medicine applications

[Excerpt] A tissue engineering approach combines the use of scaffold biomaterials, stem cells and growth factors. Bone morphogenetic proteins (BMPs) are growth factors that have sparked a great interest in tissue engineering due to their strong ability to promote new bone formation. Herein, we report the use of silk derived nanoparticles as carriers for delivery of human BMP-2. Silks are attractive biomaterials for tissue engineering due to its biocompatibility, slow biodegradability and excellent mechanical properties. Recombinant human BMP-2 was expressed in Escherichia coli and purified by affinity chromatography, showing bioactivity in human adipose stem cells. BMP2-containing silk particles were then prepared by a water-in-oil emulsion method. [...]info:eu-repo/semantics/publishedVersio

“ABORDAGENS PARA O MANEJO DE COMPLICAÇÕES OBSTÉTRICAS DURANTE O PARTO”

Effective management of obstetric complications during childbirth is crucial to ensure the safety of both mother and baby, requiring a comprehensive understanding of available practices and interventions. In this integrative review study, our aim was to investigate different approaches to managing obstetric complications during childbirth, including recent innovations and traditional practices, to provide insights into the best strategies for childbirth assistance. Methodology: To achieve this aim, we conducted an integrative review of the literature, examining relevant studies and research on the management of obstetric complications during childbirth. The search was conducted in electronic databases, including PubMed, Scopus, and Web of Science, using a combination of search terms related to the topic. Objective, Results, and Conclusion: The results of this integrative review highlighted a variety of approaches used in managing obstetric complications during childbirth, including surgical techniques, pharmacological therapies, and obstetric care protocols. We identified a growing trend towards multidisciplinary and innovative approaches, such as the use of advanced medical technologies and collaboration among interdisciplinary healthcare teams. We concluded that an integrative and up-to-date approach is essential to ensure the safety and well-being of both mother and baby during childbirth, recommending evidence-based and culturally sensitive practices to optimize obstetric outcomes.A abordagem eficaz das complicações obstétricas durante o parto é crucial para garantir a segurança da mãe e do bebê, exigindo um entendimento abrangente das práticas e intervenções disponíveis. Neste estudo de revisão integrativa, nosso objetivo foi investigar as diferentes abordagens para o manejo de complicações obstétricas durante o parto, incluindo as inovações recentes e as práticas tradicionais, a fim de fornecer insights sobre as melhores estratégias de assistência ao parto. Metodologia: Para alcançar esse objetivo, realizamos uma revisão integrativa da literatura, examinando estudos e pesquisas relevantes sobre o manejo de complicações obstétricas durante o parto. A pesquisa foi conduzida em bases de dados eletrônicas, incluindo PubMed, Scopus e Web of Science, utilizando uma combinação de termos de busca relacionados ao tema. Objetivo, Resultados e Conclusão: Os resultados desta revisão integrativa destacaram uma variedade de abordagens utilizadas no manejo de complicações obstétricas durante o parto, incluindo técnicas cirúrgicas, terapias farmacológicas e protocolos de assistência obstétrica. Identificamos uma tendência crescente em direção a abordagens multidisciplinares e inovadoras, como o uso de tecnologias médicas avançadas e a colaboração entre equipes interdisciplinares de saúde. Concluímos que uma abordagem integrativa e atualizada é essencial para garantir a segurança e o bem-estar tanto da mãe quanto do bebê durante o parto, e recomendamos uma prática baseada em evidências e culturalmente sensível para otimizar os resultados obstétricos

Ecosystem services generated by Neotropical freshwater fishes

To understand how Neotropical freshwater fishes (NFF) benefit society, we conducted a broad literature survey to (i) map and list the ecosystem services (ES) generated by these organisms, and (ii) investigate how human activities have affected the ecosystem service chain. We found sound evidence that NFF contribute directly and indirectly to the generation of several provisioning, regulating, supporting, and cultural services. Provisioning services have been widely recognized, especially those related to fisheries, but this class also included ornamental fish, genetic pools, medicines, aquaculture, and bioindicators. Other services remain less understood and largely ignored by society. Regulating services included seed dispersal, decomposition, and top-down control, while supporting services included nutrient cycling, habitat quality, and ecosystem engineering. Cultural services associated with NFF included recreational fishing, tourism, fishkeeping, education, production of scientific knowledge, in addition to values linked to traditional communities, such as local knowledge, cosmology, and existential foundations. Human activities have negatively impacted the generation of ES, especially those related to fishing and food provisioning. This review indicates that Neotropical fishes benefit society in multiple ways, but it is imperative to improve our understanding about those benefits, and to increase conservation efforts directed to this important component of global biodiversity