1 research outputs found
Application of Low-Frequency Raman Scattering Spectroscopy to Probe in Situ Drug Solubilization in Milk during Digestion
We
have recently shown that real-time monitoring of drug solubilization
and changes to solid state of the drug during digestion of milk
can be achieved using synchrotron small-angle X-ray scattering. A
complementary laboratory-based method to explore such changes is low-frequency
Raman spectroscopy, which has been increasingly used to characterize
crystalline drugs and their polymorphs in powders and suspensions.
This study investigates the use of this technique to monitor in situ drug solubilization in milk during the process of
digestion, using a lipolysis model/flow-through configuration identical
to that used previously for in situ synchrotron small-angle
X-ray scattering studies. An antimalarial drug, ferroquine (SSR97193),
was used as the model drug for this study. The Raman spectra were
processed using multivariate analysis to extract the drug signals
from the milk digestion background. The results showed disappearance
of the ferroquine peaks in the low-frequency Raman region (<200
cm–1) after approximately 15–20 min of digestion
when milk fat was present in the system, which indicated drug solubilization and
was in good agreement with the in situ small-angle
X-ray scattering measurements. This proof-of-concept study therefore
suggests that low-frequency Raman spectroscopy can be used to monitor
drug solubilization in a complex digesting milk medium because of
the unique vibrational modes of the drug crystal lattices