Staff radiation exposure in application of the PET-CT method

In my Bachelor-of Science work I have studied the issue of the PET-CT method application and its influence on the radiation exposure of nuclear medicine department staff at Faculty Hospital in Lochotín in Plzeň (FN Plzeň-Lochotín). In the opening chapter, the origin and recent developments in the nuclear medicine is reviewed with special focus on the PET history in the world, the physical principles of PET and the first installations of PET and PET-CT in Czech Republic and FN Plzeň-Lochotín. Then the issue of radiation protection is taken up including its history, the recent status including the relevant legal regulations, the basic principles of radiation protection, the physical methods of protection, exposure monitoring programmes and protection against radiation at the PET-CT section of nuclear medicine department of FN Plzeň-Lochotín. The reason why I chose this subject is the high energy radiation associated with PET-CT requiring special measures to ensure staff protection. The target of this work is comparison of the radiation exposure of the nuclear medicine department staff prior to and following introduction of the PET-CT method and verification of the organisation changes and radiation protection measures adopted. In practical part of my work I compared the values of personal dose equivalents Hp (10) detected with the department staff prior to and following the PET-CT method implementation. The values are shown in tables and graphs. The monthly dose equivalent values detected prior to and following the PET-CT equipment installation have been compared using the statistical method of double sample t-test. The conclusions of this study have been used to verify the efficiency of the radiation protection measures adopted at FN Plzeň-Lochotín, and to compare the status of affairs in Plzeň with those in the Nuclear Medicine Clinic of Medical Faculty and Faculty Hospital in Olomouc and at the PET Centre of the Na Homolce hospital in Prague

Detection of Gluten in Gluten-Free Foods of Plant Origin

The work deals with the issue of standardization and more accurate methodology for the isolation of gluten DNA in gluten-free products of plant origin, which is more demanding due to the more complex structure of plant cells. Three isolation methods were compared, of which the combination of glass and zirconium beads, Proteinase K and a commercially produced isolation kit was confirmed to be the most effective procedure. The given isolation procedure was more effective in one-component gluten-free foods, where the concentration of the obtained DNA ranged from 80.4 ± 0.7 to 99.0 ± 0.0 ng/µL. The subsequent PCR reaction revealed the presence of gluten not only in guaranteed gluten-free products (40%), but also in naturally gluten-free foods (50%). These were mainly gluten-free sponge cakes, gluten-free biscuits “Cranberries”, cocoa powder, coffee “3in1”, and instant coffee